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MSACL 2014 EU: Preliminary Conference Program

Salzburg Congress Center, AUSTRIA • September 2-5, 2014

With Thanks to Our Corporate Sponsors:
Thermo AB SCIEX Bruker Agilent Shimadzu

TUESDAY
7:30 AM
8:15 AM
WELCOME COFFEE
@ Registration Foyer
Enjoy coffee, a muffin and a chat with colleagues before the day starts.
8:15 AM
10:00 AM

SHORT COURSES: SESSION 1
Introduction to Clinical Mass Spectrometry
Judy Stone, PhD & Daniel Holmes, MD
Level: 1 (Beginner)
Location: Mozart 4
Solid Phase Microextraction (SPME) and Other Solventless Sampling and Sample Preparation Technologies for Laboratory and On-Site
Dr. Janusz Pawliszyn & Dr. Barbara Bojko
Level: 2 (Intermediate)
Location: Mozart 1
Pair this Course with How to Process Body Fluids for LC-MS/MS Analysis of Small Molecules on Wednesday for a discounted rate.
Detection of Pathogens and Toxins using Mass Spectrometry
Jean Armengaud, PhD
Level: 2 (Intermediate)
Location: Mozart 3
Concepts of Mass Spectrometry and Chromatography for Clinical and Bioanalytical Applications
Christoph Seger, PhD, Christian Huber, PhD & Christian Klampfl, PhD
Level: 3 (Advanced)
Location: Mozart 5
CANCELLED
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Papageno
10:00 AM
10:30 AM
COFFEE BREAK
@ Registration Foyer
Take a break and get a coffee, water and/or snack. Commune with colleagues or perhaps go for a short walk outside to refresh for the next session.
10:30 AM
12:00 PM

SHORT COURSES: SESSION 2
12:00 PM
1:00 PM
LUNCH
@ Ground Floor
Lunch to be provided in the Registration Foyer with the opportunity to eat inside or outside in front of the Congress Center on bistro tables.

Experience the fresh air of Salzburg. It's as clean and crisp as their tap water. Amazing!
1:00 PM
2:15 PM

SHORT COURSES: SESSION 3
2:15 PM
2:45 PM
COFFEE BREAK
@ Registration Foyer
Take a break and get a coffee, water and/or snack. Commune with colleagues or perhaps go for a short walk outside to refresh for the next session.
2:45 PM
4:15 PM

SHORT COURSES: SESSION 4
4:15 PM
6:15 PM
RECEPTION
@ Ground Floor
An evening buffet dinner with appetizers and drinks to allow you time to connect with your colleagues before heading out to explore Salzburg!
Sponsored by:
Thermo
6:15 PM
Your Decision
ENJOY THE CITY
@ Salzburg Old City
Explore the Old Town of Salzburg.
TUESDAY CLOSED

WEDNESDAY
7:30 AM
8:15 AM
WELCOME COFFEE
@ Registration Foyer
Enjoy coffee, a muffin and a chat with colleagues before the day starts.
8:15 AM
10:00 AM

SHORT COURSES: SESSION 1
Preparing Manuscripts for Publication: Improving Your Chances for Success
Thomas Annesley, PhD
Level: 0 (General Interest)
Location: Mozart 2
Introduction to Clinical Mass Spectrometry
Continued from Saturday
Judy Stone, PhD & Daniel Holmes, MD
Level: 1 (Beginner)
Location: Mozart 4
How to Process Body Fluids for LC-MS/MS Analysis of Small Molecules
Karl-Siegfried Boos, PhD
Level: 2 (Intermediate)
Location: Mozart 1
Pair this Course with Solid Phase Microextraction (SPME) and Other Solventless Sampling and Sample Preparation Technologies for Laboratory and On-Site on Tuesday for a discounted rate.
Detection of Pathogens and Toxins using Mass Spectrometry
Continued from Saturday
Jean Armengaud, PhD
Level: 2 (Intermediate)
Location: Mozart 3
Concepts of Mass Spectrometry and Chromatography for Clinical and Bioanalytical Applications
Continued from Saturday
Christoph Seger, PhD, Christian Huber, PhD & Christian Klampfl, PhD
Level: 3 (Advanced)
Location: Mozart 5
CANCELLED
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Continued from Saturday
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Papageno
10:00 AM
10:30 AM
COFFEE BREAK
@ Registration Foyer
Take a break and get a coffee, water and/or snack. Commune with colleagues or perhaps go for a short walk outside to refresh for the next session.
10:30 AM
12:00 PM

SHORT COURSES: SESSION 2
12:00 PM
1:00 PM
LUNCH
@ Ground Floor
Lunch to be provided in the Registration Foyer with the opportunity to eat inside or outside in front of the Congress Center on bistro tables.

Fresh air has been reported to assist in reduced lethargy and increased levels of Vitamin D (if accompanied by sun exposure).
1:00 PM
2:15 PM

SHORT COURSES: SESSION 3
2:15 PM
2:45 PM
COFFEE BREAK
@ Registration Foyer
Take a break and get a coffee, water and/or snack. Commune with colleagues or perhaps go for a short walk outside to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today, it should be placed by the end of this break.
2:45 PM
4:15 PM

SHORT COURSES: SESSION 4
4:15 PM
7:30 PM
OPENING RECEPTION
@ Exhibit Hall / 1st Floor
Enjoy mingling with colleagues and Exhibitors. Take time to explore the Posters, which will be attended from 5:00 - 6:00 PM.

Buffet dinner, appetizers and drinks to be provided.
Sponsored by:
Thermo
5:00 PM
6:00 PM
POSTERS
@ Exhibit Hall / 1st Floor
ALL Posters Attended from 5:00 - 6:00 PM.

POSTER PRESENTERS: Remove posters between 7:15 and 7:30 PM.
7:30 PM
Your Decision
ENJOY THE CITY
@ Salzburg Old City
Explore the Old Town of Salzburg.
WEDNESDAY CLOSED

THURSDAY
7:30 AM
8:00 AM
PLACE POSTERS
@ Exhibit Hall / 1st Floor
Poster presenters for Thursday must have their posters placed by 8 AM.
7:30 AM
8:15 AM
WELCOME COFFEE
@ Registration Foyer
Enjoy coffee, a muffin and a chat with colleagues before the day starts.
8:00 AM
8:15 PM
WELCOME, INTRODUCTION & ORIENTATION
@ Mozart Hall
Welcome, Introduction and Orientation

Welcome to the Inaugural European MSACL Congress!

Congress Mobile Apps
Updates on two conference apps that will hopefully make your contact collecting and Scientific Program browsing much easier.

(1) The Mobile Program App
Online @ https://www.msacl.org/mobile

(2) BadgeScan™ for Contact Lead Collection.

Available on Google Play and the Apple App Store (iTunes).
PLENARY LECTURE SERIES
@ Mozart Hall
Chair: David Herold
8:15 AM
9:00 AM
Direct Mass Spectrometric Characterization of Fluids, Cells and Tissues - The Benefits and the Price of Real-Time Analysis
Zoltan Takats
Imperial College London
No Long Abstract | Financial Disclosure
Development of ambient mass spectrometric techniques opened a new way of looking at clinical samples regarding the simplicity (and time demand) of analysis and the nature of data produced by these techniques. Application of these approaches in cancer surgery, histopathology, clinical microbiology and clinical chemistry has been successfully demonstrated. While these techniques mean valid alternatives to currently used technologies - with clear benefit on both cost and reliability sides - their widespread application can potentially change the current landscape of medical diagnostics. Future role of these methods in stratified clinical patient journeys will be discussed with particular emphasis on the currently unresolved problems and their future solutions regarding both regulatory and technology aspects.
9:00 AM
9:45 AM
Metabolism and Disease Pathogenesis
Gary Siuzdak
The Scripps Research Institute
No Long Abstract
Our lab focuses on the quantitative global analysis of endogenous metabolites (metabolomics) and the role these molecules play in disease pathogenesis. While the genome and proteome represent upstream biochemical events, metabolites correlate with the most downstream biochemistry and therefore most closely represent the phenotype. The experimental aim in our studies is to obtain a comprehensive view of the metabolome to expand our understanding of what pathways are altered in disease. We have developed a novel web-based platform for metabolomics that includes XCMS Online data analysis combined with METLIN, a comprehensive MS/MS metabolite database, as well as Nanostructure Imaging Mass Spectrometry (NIMS). These technologies will be presented in the context of pain and colorectal cancer.
9:45 AM
10:45 AM
COFFEE BREAK
@ Exhibit Hall / 1st Floor
Visit the Exhibit Hall to procure coffee, juice, water and/or snacks. Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk outside to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today your poster should have been up 2 hours ago. If it is not up please put it up immediately.

Sponsored by:
Thermo AB SCIEX Bruker Agilent Shimadzu
GENERAL SCIENTIFIC SESSION 1
Track 1
Mozart 1-3
Sample Prep
Chair: Karl-Siegfried Boos		Track 2
Mozart 4-5
Metabolomics
Chair: Warwick Dunn		Track 3
Papageno Hall
Standardization
Chair: Jean Armengaud
10:45 AM
11:10 AM
Immuno-MALDI for Accurate and Precise Clinical Protein Quantitation
Christoph Borchers
UVic Genome BC Proteomics Centre
Long Abstract | Bio | Financial Disclosure
Immuno-MALDI (iMALDI) technology combines the sensitivity of immunoaffinity capture with the specificity of mass spectrometry detection. We have now taken a multifaceted approach for translating iMALDI technology into clinical laboratories for routine protein quantification. First, we have automated the sample preparation using the Agilent Bravo liquid handling robot for improved sample throughput. Secondly, we have optimized iMALDI assays for the Bruker Microflex MALDI-TOF, a bench-top instrument that is widely used in regulated healthcare environments. We demonstrate that iMALDI technology is suitable for clinical use through the precise and accurate measurement of the plasma renin activity in >200 clinical samples.		Metabolic Profiling as a Tool for Investigating Diseases of Pregnancy
Elizabeth Want
Imperial College London
Long Abstract | Bio
Metabolic profiling can offer insights into disease diagnosis, progression, and responses to therapeutic intervention. Ultra-performance liquid chromatography – mass spectrometry (UPLC-MS) is a powerful tool for metabolic profiling, with excellent separation and detection capabilities. We developed a robust, reproducible UPLC-MS method for placental extract profiling and applied this to a cohort of women with intrahepatic cholestasis of pregnancy (ICP). We demonstrated clear metabolic differences between treated and untreated ICP patients, which may be reflected in plasma/serum, offering the potential for a minimally invasive diagnostic tool. This approach could be extended to study other pregnancy complications, such as pre-eclampsia and pre-term delivery. 		A New Method to Assess Sequencing & Annotation Quality in Databases Used for Clinical Proteomics and Metaproteomics
Olivier Pible
CEA/DSV/IBEB/SBTN/LBSP
Long Abstract
Advances in next-generation genome sequencing have made proteomic experiments more successful than ever. However, genome sequences are contaminated more frequently than is admitted, with large impact on most proteomics fields. Here, we propose a new concept to highlight abnormal organism sequencing data and quickly identify the source of contamination. A specific software program was developed for a quick spotting of cross-contamination of organisms, using specific experimental MS/MS data. We highlighted two likely contaminated WGS data detected in the NCBInr database and confirmed this discovery by large scale blast analysis. Other examples related to pathogens, and associated problems will be commented. Such new concept should rapidly improve the quality of sequence databases of upmost importance for clinical proteomics analysis.
11:10 AM
11:35 AM
Method Development with Easy to Automate Absorptive Chemistry Extraction for LC-MS
Roland Geyer
Tecan Switzerland AG
Long Abstract | Bio | Financial Disclosure
When using the innovative AC Extraction Plate(TM) for the extraction of small molecules, the method development process can be simplified due to the plate’s unique ‘pipette and shake’ workflow. Initial optimization simultaneously deals with three steps (extraction, wash and elution) in a matrix approach, the Direct Extraction-Elution Method (DEEM). This process evaluates the optimum conditions for the analyte(s) interaction with the absorptive chemistry (AC) of the extraction plate. Further optimization of conditions for the extraction step tackle the interaction of analyte with the sample matrix (e.g., protein binding). For analytes such as steroids (e.g., Testosterone, Androstendione, Estradiol) the extraction conditions must be modified and adapted to overcome protein binding. Experience with the optimization of this critical step will be outlined and interesting examples discussed.		Quantitative Multiplex Assays for Inborn Metabolic Disorders in Dried Blood Spots
Zdenek Spacil
University of Washington
Long Abstract | Bio
In recent years, more efficient therapies are becoming available for inherited lysosomal storage disorders (LSDs) and timely initiation of treatment often leads to a better outcome. Consequently, tandem mass spectrometry based screening assays in newborns are being increasingly implemented in clinical laboratories. We have been developing a fully quantitative assay of nine lysosomal enzymes in dried blood spots (DBS), which screens for Niemann-Pick-A/B, Pompe, Fabry, Gaucher, Krabbe and mocopolysacharidoses I; II; IVA and VI diseases. We will present our latest progress and future directions of LSDs assay development. Further, we will demonstrate the clinical value of data from LSDs pilot studies conducted by our collaborators and worldwide.		Quality Assurance in Clinical Mass Spectrometry
Michael Vogeser
Hospital of the University of Munich, Germany
Long Abstract | Bio
Among the analytical techniques used in clinical pathology today, mass spectrometry based methods potentially enable analyses on a unique level or reliability: signal generation is based on molecular weight an disintegration patterns of analytes, and application of stable isotope dilution internal standardisation suggests complete compensation of individual matrix effects. However, high complexity of the technology, the dynamic nature of analyte ionisation, highly variable instrument configurations, and incomplete solutions for automation challenge the quality of mass spectrometric methods. In this presentation specific requirements for quality assurance of mass spectrometry methods applied in clinical diagnostics regarding patients’ safety are discussed.
11:35 AM
12:00 PM
Rapid Bedside Diagnosis Tools by Coupling of Bio-compatible Solid Phase Microextraction (SPME) Devices to Mass Spectrometry
Janusz Pawliszyn
University of Waterloo
Long Abstract | Bio
SPME is a green sample preparation technique that combines extraction and pre-concentration of analytes in one step thus simplifying the analytical process. Succinctly, SPME does not require any sample collection because extraction takes place in situ by inserting a biocompatible microfiber directly into tissue, blood or other biological matrix for a short period of time. Alternatively, the same device can be used for ex vivo analysis using a small amount of the studied sample. This work presents multiple strategies recently developed for the direct coupling of SPME to MS. In order to have a broader range of applications, different SPME geometries such coated fibers and meshes, as well as ionization approaches such DART and ESI, were studied.		Study of Pregnancy Outcomes: Quantification of Selected Metabolites by High-throughput Nano-electrospray HRMS-TOF Method
Elena Chekmeneva
Imperial College London
Long Abstract | Bio
The precise, accurate and rapid validated high-throughput direct injection nano-ESI-HRMS was applied for quantification of several selected metabolites in the urine samples of the female patients with different pregnancy outcomes (Pregnant, Non-Pregnant and Early Pregnancy Loss (EPL)). The absolute quantification of eight selected metabolites was achieved by standard addition using stable isotope labelled internal standards, using a composite urine sample to account for any matrix effect. Some other metabolites were quantified relative to these internal standards. The concentration of some metabolites showed differences for different pregnancy outcomes. The full-scan data was used for untargeted fingerprinting. 		Influence of Glycosylation for Providing Relevant “clinical” Protein Calibrants
Virginie Trégoat
JRC-IRMM
Long Abstract | Bio
Sound medical decisions rely on accurate clinical measurements. To be trustable and comparable, these measurement results have to be metrologically traceable which can be established by using protein-based certified reference materials (CRMs). Many clinical diagnostic methods for proteins are based on immunoassays. Since proteins undergo post-translational modifications such as glycosylation, possible changes in glycosylation might affect protein recognition and its quantitation by immunoassays. However, this effect has hardly been studied in available CRMs. This work aims to evaluate the impact of glycosylation on the commutability and value assignment of several protein-calibrants by liquid chromatography-mass spectrometry, capillary electrophoresis and circular dichroism.
12:00 PM
1:00 PM
LUNCH
@ Exhibit Hall / 1st Floor
Lunch to be provided in the Exhibit Hall.
• Get ready to join a Corporate Workshop at 1:00 PM.
• All Posters to be attended during the 2:00 - 3:00 PM coffee break.

Sponsored by:
Thermo AB SCIEX Bruker Agilent Shimadzu
1:00 PM
2:00 PM

CORPORATE WORKSHOPS (PM)
Bruker
Mozart 1-3

Innovative Applications of Mass Spectrometry in Forensics and Clinical Research

Pre-Register

1) An Introduction to Bruker's Chromatography and Mass Spectrometry Portfolio
Joe Anacleto, Bruker Daltonics, Canada

2) MALDI Biotyper - Changing Microbiology: Detection of Resistance Mechanism
Guido Mix, Bruker Daltonik GmbH, Germany

3) Toxtyper - A New Type of Forensic Evidence
Prof. Dr. Thomas Krämer, University of Zürich, Institute of Forensic Medicine, Switzerland

4) Mass Spectrometry Meets Histology: MALDI Imaging in Clinical Research
Dr. Sören Deininger, Bruker Daltonik GmbH, Germany

Thermo Scientific
Mozart 4-5

Novel Techniques for Forensic Toxicology Screening and Sample Extraction

Pre-Register

Hear our customers present their ground-breaking work using the latest innovative approach to forensic toxicology. Reserve your seat today! Novel techniques for Forensic Toxicology Screening and Sample Extraction and Analysis Without Traditional LC for Rapid Throughput of Complex Matrices Including Blood and Urine using QQQ and HR/AM Q Exactive MS Speaker: Marta Kozak, Thermo Fisher Scientific Tox library and workflow Speaker: Bénédicte Duretz, Thermo Fisher Scientific Sample Extraction and Analysis Without Traditional LC for Rapid Throughput of oncology drug measurement with HR/AM Q Exactive MS Speaker: William Clarke, John Hopkins University

AB SCIEX
Papageno

Mass Spectrometry Applications for Clinical Research
Russell Watts & Dan Blake

Over the last 10 years many LC/MS/MS applications have been developed by clinical research laboratories and successfully implemented as the technology has become more robust, reliable and affordable. LC/MS/MS offers many technical and financial advantages for the clinical research laboratory and is now seen as a complementary and in some cases a viable alternative to immunoassays. In this workshop we bring together scientists, clinicians and biochemists that hold an interest in the use of Mass Spectrometry in the areas of Clinical Research as we discuss what can achieved with today’s technology and what we can expect in the future.

Phenomenex
Paracelsus

Chromatographic Method Development for the LC/MS Users
Dr. James Rudge

Pre-Register

The advent and rapid popularity of the MS detectors have led to shorter and narrower columns and subsequently faster chromatographic run times. This presentation covers a brief discussion of chromatographic media, bonded phases and method development in connection with LC/MS and LC/MS/MS technology and requirements.

2:00 PM
3:00 PM
COFFEE BREAK
@ Exhibit Hall / 1st Floor
Visit the Exhibit Hall to procure coffee, juice, water and/or snacks. Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk outside to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today now is the time to attend it.

Sponsored by:
Thermo AB SCIEX Bruker Agilent Shimadzu
2:00 PM
3:00 PM
POSTERS
@ Exhibit Hall / 1st Floor
All Posters to be attended from 2:00 - 3:00 PM.
GENERAL SCIENTIFIC SESSION 2
Track 1
Mozart 1-3
Small Molecule
Chair: Michael Vogeser		Track 2
Mozart 4-5
Proteomics
Chair: Christoph Borchers		Track 3
Papageno Hall
Imaging & Analytics
Chair: Axel Walch
3:00 PM
3:25 PM
TDM in Psychopharmacology Using LC-MS/MS – from the Complex Method to the Interpreted Result
Markus Schwarz
Klinikum der Universitaet Muenchen
Long Abstract | Bio
Therapeutic Drug Monitoring (TDM) is an important tool for individualized psychopharmacotherapy, allowing to get a kind of ‘pharmacologic phenotyping’ of the patient. From the methodological aspect, TDM in psychopharmacotherapy is facing one major problem: Polypharmacy, leading to the necessity to use a highly specific and at the same time robust and sensitive method. LC-MS/MS for TDM of psychotropic drugs is therefore more and more becoming the gold standard. This presentation will address the major analytical, pharmacokinetic, pharmacogenetic and practical aspects of TDM in psychiatry to demonstrate how TDM can be used for individualized risk reduction. 		MSIA Workflow for Comprehensive Identification and Analysis of Isobaric Insulins: An Approach to Targeted Insulin Quantitation Using HR/AM-MS and Multiplexed LC
Lewis Couchman
King's College Hospital
Long Abstract | Bio | Financial Disclosure
There is a trend towards the analysis of insulins using LC-MS. We report a mass spectrometric immunoassay (MSIA) method, with high-resolution, accurate mass detection (HR/AM), and chromatographic resolution of a known isobaric insulin analogue, insulin lispro. Total analysis time was 15 minutes (without multiplexing). Analytes were detected using a Q Exactive MS (Thermo Scientific). Insulin, insulin lispro, and the internal reference standard are enriched simultaneously from samples using the insulin MSIA method. Combined with HR/AM, it is possible to acquire full-scan and MS2 data for both analytes independently, allowing (i) robust peak identification, and (ii) quantitative analysis of both compounds. 		MALDI Molecular Imaging of Proteins, Metabolites and Drugs for Preclinical and Clinical Research
Axel Walch
Helmholtz Zentrum München
Long Abstract
This presentation will give an update on the application of MALDI imaging in preclinical and clinical research. We discuss the use of MALDI imaging in clinical proteomics and put it in context with classical proteomics techniques for tissue analysis. In the research area of gastrointestinal disorders MALDI imaging has already been used to address several questions of upper- and lower gastrointestinal diseases, which will be briefly presented. We also highlight a number of upcoming challenges for personalized medicine, development of targeted therapies and diagnostic molecular pathology where MALDI imaging could help.
3:25 PM
3:50 PM
CYP1A2 Phenotyping by Measuring Paraxanthine/caffeine Concentration Ratios in Hair and Comparison with the Plasma-based Phenotype
Pieter De Kesel
Laboratory of Toxicology, Ghent University
Long Abstract | Bio
Measuring metabolite-to-parent drug concentration ratios in hair may provide a convenient tool to study drug metabolism in a non-invasive way. We evaluated whether paraxanthine/caffeine ratios measured in hair samples reflect the plasma-based CYP1A2 phenotype. Using a validated LC-MS/MS method, caffeine and paraxanthine concentrations were measured in proximal 3 cm segments of hair samples from 60 healthy volunteers and resulting paraxanthine/caffeine ratios were correlated with CYP1A2 phenotyping indices measured in plasma. Although paraxanthine/caffeine concentration ratios in hair and plasma showed overall a statistically significant correlation, large deviations between hair and plasma ratios in individual cases impede interpretation.		High Selectivity and Sensitivity in LC-MS Clinical Assays
Bruno Domon
Luxembourg Clinical Proteomics Center
Long Abstract | Bio
New hybrid mass spectrometers with high resolution and accurate mass capabilities have opened new avenues in quantitative proteomics. Targeted clinical analyses, routinely performed on triple quadrupole instruments, were replicated on a high-resolution quadrupole-orbitrap instrument operated in parallel reaction monitoring (PRM) mode. The trapping capability was used to analyze peptides in tiny amounts, thus increasing the dynamic range while providing selective measurements. The PRM technique was applied to analyze makers in lung cancer markers. The gain in selectivity and an increase in the confidence of measurements in a clear discrimination of the disease stages and subtypes.		Spatial Metabolomics: Database-Driven Metabolic Annotation for High-resolution Imaging Mass Spectrometry
Theodore Alexandrov
University of Bremen / SCiLS / UCSD
Long Abstract | Bio | Financial Disclosure
High-resolution Imaging Mass Spectrometry (imaging MS) is a promising technique for untargeted spatial metabolomics. We present a novel database-driven and high-throughput approach to generate hypotheses on metabolites represented in high-resolution imaging MS data. Rather than identifying molecular species for millions of individual peaks, we restricted each imaging dataset to signals potentially corresponding to molecules from metabolic databases. Sum formulas of all metabolites were considered and corresponding ion images were generated. For each sum formula, an annotation score was calculated which integrates various spatial and spectral characteristics. Hundreds of sum formulas were detected as corresponding to metabolites present in the tissue sections. The evaluation confirmed the potential of our approach to provide relevant hypotheses on metabolites present in a tissue section.
3:50 PM
4:15 PM
Ultra-low Level Clinical Analysis Using LC-MS/MS Technologies
Russell Grant
Laboratory Corporation of America
Long Abstract | Financial Disclosure
Following the explosion of steroid hormone assays in the mid 2000's, advancements in techniques and analytical capabilities has enabled LC-MS/MS tools to supersede the performance characteristics of ELISA and RIA assays. We have leveraged these advancements to enable ultra-low level clinical biomarker analysis (<1pg/mL). This presentation will highlight the analytical challenges and solutions to realize these criterion. Specific assays for determination of estradiol/estrone (200fg/mL), free (equilibrium dialysis) and salivary Testosterone (500fg/mL), Thyroglobulin (1pg/mL peptide level) and free T3/T4 (1pg/mL)will be described. Further, multiplexing of these assays to <1500 samples/system/day will be shown.		Identification of Novel Biomarkers of Brain Injury by Integrating Bioinformatics and Mass Spectrometry-based Proteomics
Eduardo Martínez Morillo
Hospital Universitario Central de Asturias
Long Abstract | Bio | View Slides
Hemorrhagic stroke (HS) is a significant cause of mortality worldwide. A blood-based diagnostic test to identify this condition would be useful. The aim was to develop selected reaction monitoring (SRM) assays to quantify “brain specific” proteins in CSF from patients with HS, ischemic stroke and controls. SRM assays for 68 proteins were developed. Six peptides from proteins GFAP, MBP, NFM, NSE, α-Inx and β-Syn were significantly elevated in the HS group. NFM was further evaluated using an ELISA. Serum NFM concentration in controls (n=46) was from 0.26 to 8.57 ng/mL, while in 78 serial samples from 7 patients with HS was from 0.97 to 42.4 ng/mL. 		A New Approach to Biomarker Discovery in Clinical Mass Spectrometry Through Statistical Modelling of the Raw Data
Hanqing Liao
University of Manchester
Long Abstract | Bio

Replacing Andrew Dowsey.

We present a new type of workflow for differential analysis of LC-MS data in clinical discovery. The fundamental principle is to retain and model the raw data from start to finish, thus enabling detection below the limit of current software tools, and the assessment of differential expression in overlapping peptide signals. The data is analysed entirely in raw form, so that the full profile-mode MS1 signal is retained and utilised for differential quantification. Each LC-MS dataset is denoised and converted to an image, warped by a novel LC alignment stage and then statistical analysis performed directly on the set of aligned images through a Bayesian mixed-effects model. No error-prone peak detection or deconvolution is necessary. This enables our workflow to handle complex experimental designs with multiple experimental conditions, sources of variation and batch effects.
4:15 PM
6:15 PM
RECEPTION
@ Exhibit Hall / 1st Floor
Enjoy mingling with colleagues and Exhibitors. Take time to follow up on the posters, which were attended from 2-3pm.

Buffet dinner, appetizers and drinks to be provided.

POSTER PRESENTERS: Remove posters between 6:15 - 6:30 PM.

Sponsored by:
Thermo AB SCIEX Bruker
PLENARY LECTURE SERIES
@ Mozart Hall
Chair: Theodore Alexandrov
6:30 PM
7:30 PM
The Impact and Potential Consequences of Machine Intelligence on Healthcare
Randall Julian
Indigo BioSystems, Inc.
Long Abstract | Biography | Financial Disclosure
Intelligent machines teamed with experts are superior to experts working alone. This will have profound effects on the nature of healthcare delivery. Further, the advance of automation is already having a significant effect on labor markets, and there is no reason to believe healthcare will not be impacted. In this lecture examples of human-machine teams will be given. Also, the impact on society of the increased role of smart machines will be discussed. Comparisons between the first and second machine ages will be used to draw out the consequences, benefits and difficulties we will face as a scientific community.
7:30 PM
Your Decision
ENJOY THE CITY
@ Salzburg Old City
Explore the Old Town of Salzburg.
THURSDAY CLOSED

FRIDAY
7:30 AM
8:15 AM
PLACE POSTERS
@ Exhibit Hall / 1st Floor
Poster presenters for Friday must have their posters placed by 8 AM.
7:30 AM
8:15 AM
WELCOME COFFEE
@ Registration Foyer
Enjoy coffee, a muffin and a chat with colleagues before the day starts.
PLENARY LECTURE SERIES
@ Mozart Hall
Chair: Zoltan Takats
8:15 AM
9:00 AM
MALDI-TOF in Medical Microbiology
Irene Burckhardt
UniversitätsKlinikum Heidelberg, Dept for Infectious Diseases
No Long Abstract | Biography
Bacterial identification via MALDI-TOF has become state of the art during the last years. For susceptibility testing different very promising assays have been proposed. However, to routinely generate MALDI-TOF data for a complete antibiogram as we know it from agar diffusion or MIC determination is still not possible. To miniaturize these assays and to integrate them into a total lab automation will be the key tasks for the coming years.
9:00 AM
9:45 AM
High Resolution Proteomics for Clinical Applications
Matthias Mann
Max-Planck Institute of Biochemistry
Long Abstract
Mass spectrometry based proteomics has advanced tremendously in the last few years. We describe a shotgun proteomics workflow that allows us to detect and quantify the large majority of the proteins expressed in a biological system such as cancer cell lines. This included streamlined and highly efficient sample preparation, analysis with very high sequencing speed using modern mass spectrometers and bioinformatic analysis using the MaxQuant and Perseus platforms. Efforts in our group have focused on ‘single shot’ analysis and we demonstrate very high coverage in this mode (Mann et al., 2013). In this talk, I will focus on applications of proteomics to questions of clinical relevance.
9:45 AM
10:45 AM
COFFEE BREAK
@ Exhibit Hall / 1st Floor
Visit the Exhibit Hall to procure coffee, juice, water and/or snacks. Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk outside to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today your poster should have been up 2 hours ago. If it is not up please put it up immediately.

Sponsored by:
Thermo AB SCIEX Bruker Agilent Shimadzu
GENERAL SCIENTIFIC SESSION 3
Track 1
Mozart 1-3
Small Molecule/Sample Prep
Chair: Brian Keevil		Track 2
Mozart 4-5
Metabolomics
Chair: Elizabeth Want		Track 3
Papageno Hall
Novel Methodologies
Chair: Matthias Mann
10:45 AM
11:10 AM
Immunoaffinity Extraction Coupled Online with LC-MS/MS for the Quantification of Total Plasma Testosterone: A Feasibility Study
Martijn van Faassen
University Medical Center Groningen
Long Abstract | Bio
Immunoaffinity extraction was coupled online with LC-MS/MS to explore the possibility of quantifying total plasma testosterone. 25 µL plasma was analyzed using immunoaffinity- or C8-sorbents. Extraction and elution parameters were optimized. Ion suppression experiment results were comparable between immunoaffinity extraction and C8-extraction. We showed that it is feasible to combine online immunoaffinity extraction with LC-MS/MS for the determination of total plasma testosterone. Currently we are combining antibodies that capture different compounds to enable the multiplex analysis of disease specific biomarker panels. 		Investigating Beneficial Changes in Human Metabolism and Immunological and Inflammatory Markers Following High Intensity Interval Or Regular Endurance Training
Warwick Dunn
University of Birmingham
Long Abstract | Bio
Age-related declining health has a significant impact upon quality of life and healthcare costs. Exercise is a non-invasive intervention to maintain healthy status by reducing deleterious changes in immune and inflammatory status. Understanding the interactions between exercise, age and metabolism will provide insights in to how exercise interventions can maximise their effect in maintaining health as we age. The presentation will discuss a mass spectrometry metabolomics study of changes in the plasma metabolome related to two different exercise regimes, age and inflammatory/immunological status. The presentation will also discuss the importance of quality assurance and metabolite annotation in these discovery studies.		Quantitation of Soluble Transferrin Receptor (sTfR) in Human Serum Using SISCAPA Immunocapture Enrichment and MALDI-TOF Mass Spectrometry
Selena Larkin
SISCAPA Assay Technologies
Long Abstract | Bio
A sensitive, accurate, high-throughput and automated assay was developed for LC-free SISCAPA-MALDI quantification of sTfR. The traditional ELISA assay for quantitation of sTfR in human serum is suspected to be subject to protein interferences that can lead to inaccurate results. Here we present an alternative approach to the ELISA assay. This approach consists of automated digestion of the sample, including any present protein interferences, followed by automated, parallel analyte enrichment in 96-well format and quantitation using MALDI-TOF mass spectrometry. The approach was assessed for sensitivity and precision and was determined to be suitable for clinical analysis of patient samples.
11:10 AM
11:35 AM
Towards a LC-MS/MS Based Clinical-chemical Analyzer for Small Molecules in Body Fluids
Karl-Siegfried Boos
Laboratory of BioSeparation, Munich
Long Abstract | Bio | Financial Disclosure
For a broad implementation and routine application of LC-MS/MS in clinical laboratories, sample pretreatment has to be integrated and fully automated. Towards this, we developed a novel instrumental platform which – for the first time – enables a fully automated in-line processing not only of native blood plasma, blood serum, cerebrospinal fluid, saliva and urine but also of anticoagulated whole blood samples prior to on-line SPE-LC-MS/MS analysis. Perfusion through a heated stainless-steel capillary converts whole blood into so-called cell-disintegrated blood (CDB). CDB represents a homogenous liquid composed of subcellular particles which do not sediment on standing and do not clog LC-capillaries, sieves or column packings. Target analytes present in CDB and other biofluids are extracted by on-line SPE under high flow velocity conditions.		Spatial Metabolomics of Three-dimensional Cell Culture Systems
Andrew Palmer
University of Bremen
Long Abstract | Bio
3D cell cultures of colon adenocarcinoma provide an advanced in vitro model for studying metabolic processes within tumours, and their response to drug treatment. However, system-wide analysis requires untargeted detection of metabolites inside these spheroid cultures and localization to a particular spheroid layer. We addressed this analytical challenge using our newly developed spatial metabolomics approach, which combines high mass-resolution imaging mass spectrometry with high-throughput database-driven molecular annotation of imaging mass spectrometry data. Our approach allowed us to detect hundreds of signals and transform the large high mass resolution data into an easily interpretable form for experts in cancer metabolomics. 		Real-time Identification of Gastrointestinal Polyps and Other Alterations During Endoscopic Procedures: The iEndoscope
Julia Balog
Imperial College London
Long Abstract | Bio
Endoscopic screening is routinely used for the early stage detection of gastrointestinal tumours. Our aim is to create a fast, in-situ endoscopic tissue identification tool based on rapid evaporative ionization mass spectrometry (REIMS). The iEndoscope method has been shown to be capable of differentiating between healthy mucosa, cancer, adenomatous polyps and other tissue degenerations based on the REIMS fingerprint of each tissue type. The novel iEndoscope is a feasible technique for rapid identification of human tissue in-vivo during endoscopic interventions, and it can also be used as a safety tool giving a warning signal if the submucosal region becomes damaged.
11:35 AM
12:00 PM
Potassium-based Algorithm Allows Correction for the Hematocrit Bias in Quantitative Analysis of Caffeine and Its Major Metabolite in Dried Blood Spots
Sara Capiau
Laboratory of Toxicology, Ghent University
Long Abstract | Bio
Deviating Hct values may cause significant bias in quantitative dried blood spot (DBS) analysis. We evaluated whether a potassium-based algorithm allowed correction of the Hct bias, using caffeine as a model compound. An algorithm was constructed using data from a reference set of DBS and whole blood samples and applied to a separate test set. While at Hct levels below 0.36, caffeine concentrations in DBS were significantly underestimated compared with blood, this was no longer the case after application of the algorithm. The usefulness of this approach was further demonstrated by applying the same algorithm to paraxanthine, yielding similar results.		Application of Oxylipin Profiling to a Sulindac Intervention of Pain.
Jessica Miller
University of Arizona Cancer Center
Long Abstract | Bio
Despite successful use of selective estrogen receptor modulators (SERMs) and aromatase inhibitors (AIs) for the treatment of estrogen receptor positive (ER+) breast tumors, 25-30% of ER+ patients still die from their disease. High rates of early discontinuation of AIs (estimated at 30% by year 3) due to intolerance to side effects, notably musculoskeletal pain or AI-induced musculoskeletal syndrome, are now linked to reduced benefit. Here, within the context of an ongoing clinical trial using UPLC-QTRAP, we have profiled the biologically active oxylipin metabolites of Ω-6 and Ω-3 fatty acids in plasma and urine in order to understand their relationship to AI-induced pain as well as response to the pain-reducing drug, sulindac. Profiles were highly interconnected with ~1/3rd of the first set of 10 patients harboring a theoretical systemic pro-inflammatory metabolome.		Rapid Characterization and Identification of Clinically Relevant Microorganisms Using Rapid Evaporative Ionization Mass Spectrometry
Nicole Strittmatter
Imperial College London
Long Abstract | Bio
The capabilities of rapid evaporative ionization mass spectrometry (REIMS) as a general identification system for microorganisms are presented. Strains of 28 clinically relevant bacterial species were analyzed in negative ion mode and corresponding data subjected to unsupervised and supervised multivariate statistical analyses. The created supervised model yielded correct cross-validation results of 95.9%, 97.8% and 100% on species-, genus- and Gram-stain-level, respectively. Additionally, the technique proved suitable to distinguish five pathogenic Candida species with 98.8% accuracy without any further modification to the experimental workflow. Subspecies specificity is shown in case of seven Escherichia coli strains and three different Clostridium difficile ribotypes.
12:00 PM
1:00 PM
LUNCH
@ Exhibit Hall / 1st Floor
Lunch to be provided in the Exhibit Hall.
• Get ready to join a Corporate Workshop at 1:00 PM.
• All Posters to be attended during the 2:00 - 3:00 PM coffee break.

Sponsored by:
Thermo AB SCIEX Bruker Agilent Shimadzu
1:00 PM
2:00 PM

CORPORATE WORKSHOPS (PM)
Agilent
Mozart 1-3

Optimizing Mass Spec Analysis and Quantitation

Pre-Register

1) Achieve Peak Performance with Agilent Technologies
Kevin McCann, Agilent Technologies

2) Inflammatory Cytokine and Chemokine Profiles of Primary Human Cells Determined with Chip-HPLC-Triple Quadrupole Spectrometry
Christopher Gerner University of Vienna, Institute of Analytical Chemistry, Austria

3) Quality Requirements for Quantitative Clinical Chemistry Proteomics - A Proof-of-Principle Study
Christa Cobbaert, Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Center, Leiden, The Netherlands

Shimadzu
Mozart 4-5

Smart Solutions for Clinical Analysis
Giancarlo LA MARCA / Neil LOFTUS / Emmanuel WEY

Pre-Register

1) Newborn screening for ADA SCID by MS/MS
Giancarlo LA MARCA - Meyer Children's Hospital - Florence - Italy

2) Next Generation Plasma Collection Technology And Its Impact On Clinical Lc/Ms/Ms Analysis
Neil LOFTUS - Shimadzu MSBU overseas - Manchester - UK

3) The Future Role Of Maldi Mass Spectrometry In The Evolving Challenge Of Antibiotic Resistance
Emmanuel Wey MD - Royal Free Hospital, NHS Foundation Trust, London, UK

Waters
Papageno

UPLC/MS, A Versatile Tool For The Clinical Laboratory

Pre-Register

Join us for an exciting workshop!

UPLC/MS, a Versatile Tool for the Clinical Laboratory.
Chair: Benjamin Dugas, Clinical Business Development Manager, Europe & India, Waters Corporation.

1) Using Liquid Chromatography Mass Spectrometry for the Measurement of Metabolites, Protein and Enzyme Activities
Dr Robert Barouki, Professor of Biochemistry, University Paris Descartes, Head of Metabolic Biochemistry Laboratory, Necker Enfants Maladies Hospital, Paris, France

2) Development of a LC-MS/MS Method for Analysis of Steroids in Blood
Dr Maura Brambilla, Analytical Laboratory Director, Desio Hospital, Desio, Italy

IsoSciences
Paracelsus

Deficiencies of Deuterium as an Isotopic Label in MS Standards
Scott W. Landvatter, Ph.D.

Stable isotope labeled standards are a critical component in quantification of analytes by LC/MS. Deuterium (2H) is most commonly used. However, deuterium suffers from inherent drawbacks that can limit the accuracy of quantification by LC/MS. These limitations include loss of deuterium in solution and loss of deuterium under mass spec conditions. Without validation of 2H stability, results can be called into question. Second generation LC/MS standards now avoid all the drawbacks of deuterium by incorporating 13C, 15N and, if necessary, 2H in chemically stable non-exchangeable positions. This talk will focus on the limitations of deuterium in LC/MS internal standards, will give examples of inappropriate 2H internal standards and will discuss the 13C/15N labeled improved standards that are now available.
2:00 PM
3:00 PM
COFFEE BREAK
@ Exhibit Hall / 1st Floor
Visit the Exhibit Hall to procure coffee, juice, water and/or snacks. Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk outside to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today now is the time to attend it.

Sponsored by:
Thermo AB SCIEX Bruker Agilent Shimadzu
2:00 PM
3:00 PM
POSTERS
@ Exhibit Hall / 1st Floor
All Posters to be attended from 2:00 - 3:00 PM.
GENERAL SCIENTIFIC SESSION 4
Track 1
Mozart 1-3
BioMarkers
Chair: Bruno Domon		Track 2
Mozart 4-5
Metabolomics
Chair: Oleg Mayboroda		Track 3
Papageno Hall
Toxicology
Chair: Judy Stone
3:00 PM
3:25 PM
A Proteogenomic Strategy for Defining Biomarkers for Quick Identification of Francisella Subspecies by MALDI-TOF MS
Jean Armengaud
CEA
Long Abstract | Bio
The Francisella tularensis pathogen is the causative agent of tularemia and a potential bioweapon of category A due to its high virulence. By means of an original proteogenomics strategy relying on a large panel of genomic data on Francisella bacteria and the combination of shotgun proteomics and whole-cell MALDI-TOF mass spectrometry, we established that a unique set of three protein biomarkers could enable the identification of Francisella species and subspecies, thus predicting its virulence level. Their detection as intense peaks in the most virulent subspecies of Francisella confirmed the validity of this approach that could be extended to any pathogens. 		Rapid Quantification of Cortisol, Cortisone, Dexamethasone and Prednisolone in Human Saliva and Hair by Liquid Chromatography – Tandem Mass Spectrometry
Alexander Gaudl
Leipzig University
Long Abstract | Bio
Reliable determination of cortisol and cortisone concentration in saliva and hair is of interest in the assessment of stress-associated adrenocortical function. To this purpose we developed a robust method for the reliable, simultaneous quantification of glucocorticoids via LC-MS/MS, overbearing the shortcomings of immunological analysis. With excellent precision (2-7%), very good recovery rates (95-115%) and a total runtime of 4.5 min we proved the benefits of LC-MS/MS over immunoassays. Moreover, we analysed 2500 samples of the LIFE Child Depression study and demonstrated the massive impact of preanalytical factors on hair analysis, enabling proper evaluation in clinical routine diagnostics and epidemiological studies.		Five Years of Urinary Screening for Drugs of Abuse by Tandem Mass Spectrometry and the Evolution to Pain Management Testing for Compliance and Diversion
Jeff Eichhorst
Saskatchewan Disease Control Laboratory
Long Abstract | Bio
We have been providing high volume drugs of abuse screening by tandem mass spectrometry for over 40 different drugs/metabolites to clinicians for more than five years. For the last few years, physicians have been asking for changes to our program, which would provide them improved information about low dose opioid/synthetic pain medication usage, compliance and possible diversion detection. This is logical given that the greatest increase in drug misuse is of prescription drugs; specifically pain medications. The major obstacles to this request were establishing lower, yet reliable cut-off values and providing quantitative data over a very broad dynamic range.
3:25 PM
3:50 PM
Identification of Noninvasive Biomarkers of Coordinate Metabolic Reprogramming Associated with Colorectal Cancer
Soumen Manna
National Cancer Institute, USA
Long Abstract | Bio
Tandem tissue and urinary metabolomic profiling revealed early noninvasive biomarkers of colorectal tumorigensis in ApcMin/+ mice. Transcriptomic analysis in ApcMin/+ and mice with colon-specific disruption of Apc gene showed that these biomarkers were reflection of coordinate reprogramming metabolic pathways including methylation during colorectal carcinogenesis. AOM-induced colorectal carcinogenesis mouse model showed that such metabolic reprogramming-associated biomarkers were a conserved feature of colorectal carcinogenesis in mice irrespective of etiology and genetic background. Analysis of paired non-tumor and tumor tissues from colorectal cancer patients showed that such metabolic derangements are also associated with human colorectal carcinogenesis in stage-dependent manner.		Profiling Thiol Metabolites and Quantification of Cellular Glutathione Using FT-ICR-MS
Sadakatali S. Gori
University of Louisville
Long Abstract | Bio
We describe preparation and use of the quaternary ammonium-based α-iodoacetamide QDE and its isotopologue *QDE as reagents for chemoselective derivatization and analysis of cellular thiols using FT-ICR-MS. Examination of A549 human lung adenocarcinoma cells using this approach revealed cysteine, cysteinylglycine, glutathione and homocysteine as principal thiol metabolites as well as the sulfinic acid hypotaurine. The method was readily applied to quantify the thiol metabolites glutathione and glutathione disulfide in A549 cells and the concentrations were found to be 34.4 ± 11.5 nmol/mg protein and 10.1 ± 4.0 nmol/mg protein, respectively. 		Detection of Allenic Norleucine, a Nephrotoxic Amino Acid from Amanita Smithiana and Related Mushrooms
Daniel Holmes
St. Paul's Hospital, Vancouver
Long Abstract | Bio | Financial Disclosure | View Slides
Amanita smithiana is a poisonous mushroom, causing acute renal failure, that grows along the Pacific coast of North America. Several European Amanita species are thought to share this same toxin, a non-protein amino acid called allenic norleucine. Previously, thin layer chromatography was the method used to confirm presence of the toxin, however, this was labour-intensive and subjective in its interpretation. We have now developed an LC-MS/MS method for allenic norleucine. Apart from its potential clinical utility in poisoning cases, we have used the assay to screen a large number of mushroom species for the toxin.
3:50 PM
4:15 PM
Touch Spray Mass Spectrometry (TS-MS) Used for Rapid Diagnosis of Kidney and Prostate Cancer Using Tissue Specimen Obtained from Surgery
Kevin Kerian
Purdue University
Long Abstract | Bio
Touch spray uses a small probe to pick up sample and an application of voltage and solvent to cause field-induced droplet emission for MS analysis. TS was used in a study of 18 prostatectomy cases to differentiate prostate cancer and healthy tissue with 95% accuracy based on initial cross validation results of the first 12. A blind validation was performed on the latter six patients to confirm TS as a possible in vivo surgical tool. For a professional assessment of TS-MS, Dr. Timothy Masterson performed the technique in vitro on a radical nephrectomy specimen targeting diseased and normal tissue.		A Protective Lipidomic Biosignature Associated with a Balanced Omega-6/omega-3 Ratio in Fat-1 Transgenic Mice
Giuseppe Astarita
Georgetown University
Long Abstract | Bio | Financial Disclosure
A balanced omega-6/omega-3 polyunsaturated fatty acid (PUFA) ratio has been linked to health benefits and the prevention of many chronic diseases. Current dietary intervention studies with different sources of omega-3 fatty acids (omega-3) lack appropriate control diets and carry many other confounding factors derived from genetic and environmental variability. In this study, we used a multi-platform lipidomic approach to phenotype the molecular phenotype of plasma samples from an animal model of long term omega-3 supplementation. Integration of the results of untargeted and targeted analyses has identified a lipidomic biosignature that may underlie the healthful phenotype associated with a balanced omega-6/omega-3 ratio, and can potentially be used as a circulating biomarker for monitoring the health status and the efficacy of omega-3 intervention in humans.		On the Possibility of Using Exhaled Breath for Toxicological Investigations
Olof Beck
Karolinska University Hospital
Long Abstract | Bio
Exhaled breath has recently been proposed as a matrix for drug testing. A serie of investigations have demonstrated that most common drugs of abuse are detectable in breath following intake. The normal breathing process creates aerosol micro-particles that are exhaled in breath. These patrticles are formed from the airway lining fluid during the normal breathing process. The airway lining fluid can become contaminated with drugs present in the body. These aerosol particles constitutes a way for non-volatile compounds to exit lung. This presentation will give an update on the present status in the field of drug testing in exhaled breath.
4:15 PM
6:15 PM
CLOSING RECEPTION
@ Exhibit Hall / 1st Floor
Enjoy some last bits of time mingling with colleagues and Exhibitors. Follow up on the posters, which were attended from 2-3pm. Contemplate attending MSACL 2015 US in March.

Buffet dinner, appetizers and drinks to be provided.

POSTER PRESENTERS: Remove posters between 6:15 - 6:30 PM.

Sponsored by:
Thermo AB SCIEX Bruker
FRIDAY CLOSED