As a PhD student, my project is mainly about Mass Spectrometry Imaging (MSI) on Soft Tissue Sarcoma (STS). The combination of high heterogeneity with their rarity (comprising just 1% of all malignant tumors) has made diagnosis, prognosis, staging and treatment of STS difficult. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is a successful tool for the direct molecular assessment of tissue samples because this technique is label-free, no a-priori knowledge needed, and high-throughput (able to simultaneously measure hundreds of biomolecular ions). Spatially-correlated analysis, mass spectrometry imaging, can reveal how each of these biomolecular ions varies in tissue sections. We are interested in biomarker discovery on STS using MALDI-MSI from protein level and metabolite level.