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MSACL 2016 US: Preliminary Conference Program

Palm Springs, CA • February 21 - 25, 2016

With Thanks to Our Corporate Conference Sponsors:

And Special Thanks to Our Travel Grant Sponsors:

SUNDAY

SHORT COURSES: Group A
10 minute Coffee Breaks at every :50, except before lunch
Lunch 12:00 - 1:00 PM at Date Restaurant
Ends at 5:00 PM or discretion of Instructor.

SHORT COURSES: Group B
10 minute Coffee Breaks at every :20, except before lunch
Lunch 12:30 - 1:30 PM at Date Restaurant.
Ends at 5:30 PM or discretion of Instructor.

SHORT COURSES: Group C
10 minute Coffee Breaks at every :50, except before lunch
Lunch 1:00 - 2:00 PM at Date Restaurant.
Ends at 6:00 PM or discretion of Instructor.

MONDAY

SHORT COURSES: Group A
10 minute Coffee Breaks at every :50, except before lunch
Lunch 12:00 - 1:00 PM at Date Restaurant
Ends at 5:00 PM or discretion of Instructor.

SHORT COURSES: Group B
10 minute Coffee Breaks at every :20, except before lunch
Lunch 12:30 - 1:30 PM at Date Restaurant.
Ends at 5:30 PM or discretion of Instructor.

SHORT COURSES: Group C
10 minute Coffee Breaks at every :50, except before lunch
Lunch 1:00 - 2:00 PM at Date Restaurant.
Ends at 6:00 PM or discretion of Instructor.

Appetizers and Drinks to be provided.

TUESDAY

CORPORATE WORKSHOPS (AM)

(1) Advances in MALDI Imaging of Clinical Samples, (2) The Chemistry of the Human Lung Associated with Cystic Fibrosis
(1) Jeffrey Spraggins, PhD, Vanderbilt, (2) Pieter Dorrestein, PhD, University of California, San Diego

(1) In recent years, instrumentation for imaging mass spectrometry has reached unprecedented speed and sensitivity. Bruker’s rapifleX MALDI Tissuetyper, a next-generation MALDI TOF mass spectrometer was designed for imaging on clinical timescales. This presentation will review some significant advances and demonstrate applications for the imaging of clinically important tissue specimens.
(2) The microbiome is critical to human health yet we know little about the chemical environment that our microbes live in. In this presentation we will explore untargeted metabolomics strategies to reveal the chemical environment of the microbiome. In this presentation we will highlight the metabolome of a human lung associated with Cystic Fibrosis in 3D and its relationship to medicines, metabolism of medicines and microbes .

Selectivity Accelerated: Utilizing Raptor™ LC Columns for Clinical Solutions
Frances Carroll, Shun-Hsin Liang, Sharon Lupo

Superficially porous particles (SPP) have been proven to provide fast separations without the need for expensive instrumentation, thereby increasing sample throughput without capital investment. Although column efficiency considerably accelerates analysis time, it has little effect on resolution. Conversely, selectivity has a substantial impact on resolution, but shows minimal improvement in analysis times. By being the first to combine the speed of SPP with the resolution of Restek’s unique Ultra Selective Liquid Chromatography technology, Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast and efficient method development. This workshop will discuss how to capitalize on the selectivity of Raptor™ LC columns in challenging clinical analyses by providing application examples.

Towards Standardization in Protein Quantification Workflows
Paula M. Ladwig, MS, MT(ASCP); Development Technologist Coordinator with Department of Laboratory Medicine & Pathology at Mayo Clinic in Rochester MN, Mary Lame, Senior Applications Scientist, Waters Corporation

This workshop highlights the implementation and benefits of generic, kitted methods used to simplify, streamline, and standardize common protein quantification workflows, while reducing variability and delivering accurate and precise results. Data for infliximab, adalimumab, trastuzumab, bevacizumab, and the antibody drug conjugate T-DM1 will be shown as working examples. For full abstract go to: www.waters.com/msacl

Explore what"s on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today your poster should have been up 2 hours ago. If it is not up, please put it up immediately.You will be attending your poster for 1 hour at either 3PM or 5 PM.

Seating provided in Exhibit Hall, Lobby, Esplanade AND the East Lawn next to SmokeTree.

• Get ready to join a Corporate Workshop at 2:00 PM.

CORPORATE WORKSHOPS (PM)

Alternate Sample Preparation Approaches for Your Mass Spectrometry Clinical Research Assays
William Clarke, PhD, The Johns Hopkins University School of Medicine

Pre-Register

The first part of this session will focus on the research of cell disintegrated blood (CDB) as a matrix for the analysis of immunosuppressant drugs. Opportunities, challenges, and limitations of CDB will be explored. The second part of this session will focus on alternate sample preparation approaches for the analysis of dried urine spots. Research analysis of field samples from HIV prevention trials compared to research analysis of liquid urine will be discussed, as well as other potential opportunities and limitations of the methodology.

Development of High Sensitivity MicroLC-MS/MS Method for Estradiol Research in Human Serum Without Derivatization
Jerry Yeo, Ph.D, Xin Yi, Ph.D

During this presentation, we will discuss the technical challenges of developing high sensitivity LC-MS/MS methods for estradiol in blood, how to improve sensitivity and reduce ion suppression for estradiol on MicroLC-MS/MS system, and discuss the advantages and disadvantages of using microLC system versus normal flow LC system

The Application & Benefits of Integrating Volumetric Absorptive Microsampling (VAMS) into the Clinical Lab : 1) Automated, Bottom-up Proteomics Workflow 2) LC-MS/MS Assay for Immunosuppressant Monitoring 3) Monitoring of Therapeutic Drug by LC-MS/MS & Diagnostic Biomarkers by ELISA
1) Irene van den Broek, PhD, Cedars-Sinai Medical Center; 2) Paul J. Jannetto, PhD, Mayo Clinic; 3) Ying Qu, PhD, Exagen Diagnostics

Pre-Register

Talk 1) discusses application of VAMS for protein biomarkers, focusing on the optimization of protein extraction from 10µL of blood & integration into a bottom-up proteomics workflow on an automated liquid handler. Talk 2) will look at feasibility of measuring Tacrolimus and Cyclosporin A with a HPLC-MS/MS assay using dried blood from a VAMS device (20µL) compared to a validated method using 200µL venous collected EDTA whole blood. Talk 3) will review a DBS comparative study (whole blood & red blood cell results) where VAMS (10µL) was applied in monitoring methotrexate polyglutamates (MTXPG1-5) in methotrexate (MTX) treated patients by a LC-MS/MS method as well as discuss how VAMS was used in ELISA based biomarker monitoring of rheumatoid factors (e.g. anti-IgM RF, anti-CCP) used as diagnostic biomarkers for autoimmunological disease.

Streamlined Sample Preparation of Biological Samples Using the Thomson Filter Vials & Analysis by LC-MS/MS
Lisa Wanders

Sample preparation continues to be a critical factor in the quantitative measurement of biological samples. The goal of this seminar is to discuss how to streamline the sample preparation process of oral fluids, urine and blood utilizing the Thomson eXtreme, nano and the eXtractor3D Filter Vials. Reducing interferences from sample matrices and increasing analyte recovery are key requirements for preparing biological samples. Thomson Filter Vials save time, reduce solvent usage, alleviate the need for expensive consumables and lab equipment. Sample preparation for matrices such as urine, blood and oral fluids will be discussed.

New technology for imaging of biological tissues by MALDI-TOF
Stephen Hattan

This novel approach improves sensitivity, image resolution, data quality, consistency, reproducibility, flexibility, protocol simplicity and cost of analysis for MALDI-IMS. It uses chemically modified µ-channel plates to act as substrates, and simplifies experimental protocol by removing restrictions for cryogenically sliced tissue specimens.

Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, check out the posters, or go for a short walk to refresh for the next session.

Appetizers and Drinks to be provided.

All posters to be removed by 7:30 PM.

DISCUSSION GROUPS
@ Rooms around Ballroom Foyer

Yo ho ho mateys’ – do you know your ragged ends from your missed cleavages? Full proteins or peptides for internal standardization? If not… it’s time to charge the guns and hoist the mainsail. The evenings’ entertainment will focus on bottom up protein assays through finding the flaws in proposed methods. Let the grog flow (yes there will be grog) and battle(ships) commence as we learn how to eat a whale of a “clinical protein” assay, one bite at a time.

Exhibitors, let MSACL know your thoughts on the way the conference is working for you and what we can do to make the experience better in the long run.

Participants will discuss how CDC Standardization Programs support laboratories with improving measurements for key hormones such as 25-hydroxyvitamin D, estradiol, and testosterone. Included will be additional discussions about new projects and tools available in CDC Standardization Programs.

Assemble highlights for the purpose of creating compelling powerpoint for those new to the field (i.e. clinical lab directors, managers, public). Discuss MS-based methods with respect to their technical and economic superiority/inferiority to the existing/emerging alternatives. Identify examples (if any) existing or emerging diagnostic/prognostic tests where MS is the only viable option. Highlight green aspects comparing environmental footprints (energy, toxic waste, chemical and bio-hazards) vis-a-vis existing techniques. Review evolution and adoption of Clinical MS, explore challenges, ethics, mistakes.

Feedback on the Fundamentals / Newbies track. How is it going? What can be improved?

This discussion group will review real world patent claims in the field of metabolite analysis using mass spectrometry and some simpler ‘made-up’ claims to help scientists understand what activities would fall under the experimental use doctrine, direct infringement, indirect infringement, contributory infringement, infringement under the doctrine of equivalents and how to design around infringement of a method or a device claim.

A fun-filled opportunity to apply mass spectrometry tools and talents to solve a case of mysterious exposure to chemicals of medical significance. Crime scene data can include GC-MS results and olfactory clues. Clinical analysis results can include; clinical presentation, symptoms, GC-MS, ICP-MS and/or LC-MS data. This is an opportunity to flaunt you MS interpretation skills in front of your peers.

To create the opportunity for clinical mass spectrometrists, early in their careers, to assemble with the intent of creating and maintaining an interest group with a discernible voice that will communicate directly and effectively with MSACL, MSACL vendors and the clinical mass spectrometry community at large.

WEDNESDAY

CORPORATE WORKSHOPS (AM)

Quantitative MALDI-TOF mass spectrometry for clinical applications
Marvin L. Vestal PhD

MALDI-TOF MS is used for analyzing a variety of nonvolatile molecules, but acceptance has been limited by the belief that MALDI-TOF is not quantitative. Results are presented on new MALDI-TOF mass spectrometers that generate reproducible spectra on complex samples. High sensitivity and accurate masses are achieved by operating at laser rates up to 5 kHz and summing up to 500,000 shots/sample. Recent and potential clinical applications are reviewed.

The Lipidyzer: The Convergence of Differential Ion Mobility and Mass Spectrometry to the Next Generation Lipidomics Analysis Platform
Paul Baker, Ph.D

There is growing interest in characterizing lipid molecular species as clinical biomarkers of human disease. MS-based lipidomics data arrays are processed by lipid ID software followed by PCA to generate candidate lipid biomarkers. This process is challenging due to the numerous lipid isobars that interfere with quantitation. Differential Mobility Spectrometry (SelexION® Technology) coupled to MS analysis is effective at resolving complex lipid mixtures. This technology has been applied to quantitative lipidomics using the LipidyzerTM, which detects and measures over 1200 lipids with absolute quantitation. In this presentation, the application of SelexION® Technology to global and targeted lipidomics will be discussed, and data will be shown demonstrating how it has fundamentally changed the future of lipid analysis.

Which Chromatography and Mass Analyzer is Right for Your Clinical Research Application?
Lewis Couchman, Kings College Hospital

Pre-Register

Mass spectrometry has wide applicability in clinical research, especially when combined with modern chromatography and sample preparation. However, clinical research method development should remain application-centered, and ideally use a range of approaches to design a ‘fit-for-purpose’ methodology.We will describe the applicability of turbulent flow chromatography, high-resolution, accurate mass (HRAM) LC-MS, and rapid quantitative analysis using triple quad mass analyzers. The merits of each will be discussed using examples from clinical research methods.

Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today your poster should have been up 2 hours ago. If it is not up, please put it up immediately. You will be attending your poster for 1 hour at either 3PM or 5 PM.

Seating provided in Exhibit Hall, Lobby, Esplanade AND the East Lawn next to SmokeTree.

• Get ready to join a Corporate Workshop at 2:00 PM.

CORPORATE WORKSHOPS (PM)

The Impact of Evolving LC-MS/MS Technologies upon Clinical Research Methods
Tim Wood, Biochemical Genetics Laboratory, Sarah Young, Duke Hospital Biochemical Genetics Lab

As the performance of all aspects of LC-MS/MS technology has progressed over the years, clinical research methods have benefited from increasing analytical sensitivity, specificity and throughput. In turn, this has lead to the sharpening of approaches for the detection of disease-specific biomarkers, and ultimately a better understanding of the underlying biochemistry for a number of metabolic disorders. In this workshop, you will hear experiences from two prominent experts in the biochemical genetics community who will describe the effects of evolving LC-MS/MS technology upon their research. For full abstract go to: www.waters.com/msacl

1. Spitting in the Face of your MS, and Why That is a Bad Idea. 2. Automation in the LCMS Laboratory
1. Sean Orlowicz, Seyed Sadjadi - Phenomenex 2. Rohit Shroff - Tecan

1. Oral Fluids provide many advantages over more invasive sample collection options, however their LCMS analysis can come with litany of new challenges. In this talk, we focus on overcoming some of these challenges including; sensitivity, matrix effects and the recovery of multiple drug classes, through the use of sample preparation and HPLC techniques. 2. Increasing test volumes of mass spectrometry assays in the clinical laboratories are challenging many to revisit the major bottleneck in their workflows – manual sample preparation. Tecan has developed tailored workflow automation solutions for a variety of clinically important analytes and extraction methodologies to alleviate this bottleneck. In this talk, we will investigate some of the tools available to improve your workflow.

Small Sample Volumes Are Small Problems
Kris Franklin, Lite Consulting

With advances in LC-MS/MS technology and automated sample preparation, the need for large sample volumes are no longer a requirement for many assays. The sensitivity and specificity of modern LC-MS/MS allows for multi-analyte panels to be targeted, which has the potential to reduce cost for clinical lab operation. The challenge of multiplexing targets into a single LC-MS/MS run is the development of a robust sample prep that sufficiently removes sample matrix and interfering substances or buffers that may harm or reduce sensitivity of the instrument, while maintaining adequate recovery of the targets. Recent developments in our lab have allowed for reduced sample volumes while producing clinically relevant values for both toxicology and endocrinology. This seminar will include several application examples including oral fluids, urine and hormone quantification in whole blood.

MS in the Clinical Laboratory: Roles, Challenges, Processes, and the Future
Paul J. Jannetto, Ph.D., DABCC, FACB, M.T.(ASCP), Consultant, Mayo Clinic, Department of Laboratory Medicine and Pathology, Rochester, MN

Pre-Register

This talk will explore the increasing role of Mass Spectrometry in clinical laboratories; focusing on the challenges (economic, regulatory, and validation) and how these challenges may be addressed using the Mayo test life cycle. We will also get a glimpse into future applications and full laboratory automation.

Managing Matrix Impact: High -throughput 25-hydroxy Vitamin D Testing with LC-MS/MS with Phospholipid Depletion
Judy Stone and Craig Aurand

Craig Aurand will cover the impact of phospholipid matrix interference and describe techniques to minimize the impact on LC/MS assays. Judy Stone will describe the development of a high-throughput 25-hydroxy Vitamin D (VITD) clinical assay at the Northern California Kaiser Regional Laboratories. In 2010 their test volume for serum increased 30-40% per year, and by 2011, the lab anticipated receiving 1200-1500 samples a day for this test. Stone will describe the rationale and methodology for use of Hybrid-SPE plates for VITD sample prep to add the analytical and process robustness that was considered critical for such an ambitious change in workflow to succeed. By 2015 the lab was testing for VITD at the max LC-MSMS throughput of 2200 samples a day with this protocol, maintaining excellent performance metrics for accuracy and precision.

Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk to refresh for the next session.

Appetizers and Drinks to be provided.

All Posters should be removed by 7:30 PM.

CORPORATE WORKSHOPS (Evening)

(1) Project Management for Method Validation (2) Simplifying Method Validation; Benefits of a Kitted Solution
(1) James Byrd, Project Manager & Application Chemist, Zef Scientific, Inc. (2) François Espourteille, Founder, Comprehensive LCMS Solutions

Pre-Register

(1) Preparing for a full method validation can be a daunting task. Join us to see how the tools & techniques of Project Management can make the process more predictable, more efficient, and more flexible. Example forms will be provided to the first 40 registrants. (2) Installation and validation of an assay is a complex process. We will discuss how incorporating a kitted solution removes some of the hurdles of setting up and validating an assay.

THURSDAY

CORPORATE WORKSHOPS (AM)

#1 - Preparing Clinical Samples for Meaningful Mass Spectrometry (MS) Up to 75% of the work and expenditure of an analytical lab is spent on sample preparation.
Ivona Strug

Choosing the right sample prep methods tools can improve the MS workflow and save time. Attend our popular workshop on contaminants and matrix components that potentially interfere with MS analysis, and how you can minimize interference with proper sample preparation. Some of the most expensive errors in MS analyses are caused by sample particulates. Particulates are usually removed by filtration, which could introduce further contamination if incompatible or low-quality filter devices are used. Our presentation will focus on practical solutions for sample and solvent filtration--how to choose the right filter and avoid leached contaminants. You will learn tips for simple, fast sample preparation using centrifugal devices (FASP, or filter-aided sample prep). We will also show how to make your processed samples more LC-MS-ready by applying ZipTip® devices.

: Hamilton Robotics: Innovative High-throughput Sample Preparation Modules for Bioanalytical Analysis
Michael Mouradian, Ph.D. Scientific Leader, Drug Discovery, Hamilton Robotics

This workshop will discuss Hamilton’s automation tools for bioanalytical workflows focusing on clinical laboratories. Workshop highlights will include, new modules for automated mass spectrometry sample preparation and robust sample preparation methods for reliable, high-throughput analysis.

Pharmacogenomics- and Therapeutic Drug Monitoring- Guided Treatment for Precision Patient Care.
Kevin Rosenblatt, MD, PhD, CMO, CSO & Lab Director, CompanionDx Labs

This interactive workshop will take a closer look at the use of genetics, metabobolomics and proteomics measurements to suggest more effective patient therapies. Successful integration and reporting of pharmacogenomics and metabolite/'omics data, as well as the development of new MS-based technologies that enable these measurements, will be presented. Advances in ultra-fast single reaction monitoring/multiple reaction monitoring (SRM/MRM), plasma separation cards (noviplex cards, Novilytic Labs) and fully-automated clinical lab automation module (CLAM-2000) technology will be discussed. "Kick Some Mass" t-shirts will be given to the first 70 attendees!

Enjoy patio seating outside overlooking the pool.