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MSACL 2016 US: Preliminary Conference Program

Palm Springs, CA • February 21 - 25, 2016

With Thanks to Our Corporate Conference Sponsors:
Thermo
Shimadzu  SCIEX
Indigo  Agilent  SimulTOF
Bruker

And Special Thanks to Our Travel Grant Sponsors:
Thermo
Sigma-Aldrich Waters Agilent
MS3   

SUNDAY

6:00 AM
7:00 AM
YOGA
@ San Jacinto

Energize yourself for the day! Yoga is a complimentary offering for all MSACL registrants.
A limited number of yoga mats will be provided.
6:00 AM
9:00 AM
BREAKFAST
@ Date Restaurant

MSACL covers Short Course registrants who have voucher collected during registration.
8:00 AM
5:00 PM

SHORT COURSES: Group A
10 minute Coffee Breaks at every :50, except before lunch
Lunch 12:00 - 1:00 PM at Date Restaurant
Ends at 5:00 PM or discretion of Instructor.

Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Jack Henion, PhD
Level: 2-3 (Intermediate - Advanced)
Location: Mojave Learning Center
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD, Lorin Bachmann, PhD & Grace Van Der Gugten
Level: 1-2 (Beginner - Intermediate)
Location: Pueblo
LC-MSMS 102
Intro to Clinical MS Method Development
Robert Kobelski, PhD
Level: 1-2 (Beginner - Intermediate)
Location: SmokeTree D
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Pasadena
8:30 AM
5:30 PM

SHORT COURSES: Group B
10 minute Coffee Breaks at every :20, except before lunch
Lunch 12:30 - 1:30 PM at Date Restaurant.
Ends at 5:30 PM or discretion of Instructor.

Presentations 102
How to Maximize Your Influence Through Creating Compelling Presentations
Karen Mahooti, MBA
Level: 1-2 (Beginner - Intermediate)
Location: SmokeTree E
Proteomics 101
Introduction to Quantitative Proteomics
Mike MacCoss, PhD & Michael Bereman, PhD
Level: 1-2 (Beginner - Intermediate)
Location: Catalina
9:00 AM
6:00 PM

SHORT COURSES: Group C
10 minute Coffee Breaks at every :50, except before lunch
Lunch 1:00 - 2:00 PM at Date Restaurant.
Ends at 6:00 PM or discretion of Instructor.

Data Science 101
Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Stephen Master, MD PhD (TA: Shannon Haymond, PhD)
Level: 1-2 (Beginner - Intermediate)
Location: Madera
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
Level: 2 (Intermediate)
Location: Sierra
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD, Erik J. Soderblom, PhD & Chris Shuford, PhD
Level: 2 (Intermediate)
Location: SmokeTree A-B
MALDI 102
Practical Considerations for MALDI Imaging Mass Spectrometry
Michelle Reyzer, PhD
Level: 1-2 (Beginner - Intermediate)
Location: SnowCreek
MALDI 201
Quantitative MALDI-TOF Mass Spectrometry for Clinical Applications
Marvin Vestal, PhD, Mark Duncan PhD, Ken Parker, PhD & Steve Hattan, PhD
Level: 2 (Intermediate)
Location: Andreas
6:00 PM
8:00 PM
PRIVATE: Travel Grantee Dinner & Reception
@ Date Restaurant & Patio

** This event is exclusively for Travel Grantees. **
Dinner and Reception from 6:00 - 8:00 PM.
Entry Ticket Required.
8:00 PM
10:00 PM
HOSPITALITY
@ Rocks Lounge

Enjoy the Palm Springs atmosphere on the patio overlooking the pool, with fire pits.
Drinks and appetizers provided.
10:00 PMSUNDAY CLOSED

MONDAY

6:00 AM
7:00 AM
YOGA
@ San Jacinto

Energize yourself for the day! Yoga is a complimentary offering for all MSACL registrants.
A limited number of yoga mats will be provided.
6:00 AM
9:00 AM
BREAKFAST
@ Date Restaurant

MSACL covers Short Course registrants who have voucher collected during registration.
8:00 AM
5:00 PM

SHORT COURSES: Group A
10 minute Coffee Breaks at every :50, except before lunch
Lunch 12:00 - 1:00 PM at Date Restaurant
Ends at 5:00 PM or discretion of Instructor.

Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Continued from Sunday
Jack Henion, PhD
Level: 2-3 (Intermediate - Advanced)
Location: Mojave Learning Center
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Continued from Sunday
Judy Stone, PhD, Lorin Bachmann, PhD & Grace Van Der Gugten
Level: 1-2 (Beginner - Intermediate)
Location: Pueblo
LC-MSMS 102
Intro to Clinical MS Method Development
Continued from Sunday
Robert Kobelski, PhD
Level: 1-2 (Beginner - Intermediate)
Location: SmokeTree D
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Continued from Sunday
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Pasadena
8:30 AM
5:30 PM

SHORT COURSES: Group B
10 minute Coffee Breaks at every :20, except before lunch
Lunch 12:30 - 1:30 PM at Date Restaurant.
Ends at 5:30 PM or discretion of Instructor.

Metabolomics 301
Metabolomics
Gary Patti, PhD
Level: 3 (Advanced)
Location: Chino
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: 2-3 (Intermediate - Advanced)
Location: Catalina
Sample Prep 201
Practical Introduction to Sample Preparation for Clinical LC/MS/MS
Hesham Ghobarah
Level: Beginner / Intermediate
Location: Ventura
Toxicology 101
General Toxicology
Jeffery Moran, PhD
Level: 1 (Beginner)
Location: SmokeTree E
9:00 AM
6:00 PM

SHORT COURSES: Group C
10 minute Coffee Breaks at every :50, except before lunch
Lunch 1:00 - 2:00 PM at Date Restaurant.
Ends at 6:00 PM or discretion of Instructor.

Data Science 101
Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language
Continued from Sunday
Daniel Holmes, MD & Stephen Master, MD PhD (TA: Shannon Haymond, PhD)
Level: 1-2 (Beginner - Intermediate)
Location: Madera
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Continued from Sunday
Robert D. Voyksner, PhD
Level: 2 (Intermediate)
Location: Sierra
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
Continued from Sunday
J. Will Thompson, PhD, Erik J. Soderblom, PhD & Chris Shuford, PhD
Level: 2 (Intermediate)
Location: SmokeTree A-B
MALDI 102
Practical Considerations for MALDI Imaging Mass Spectrometry
Continued from Sunday
Michelle Reyzer, PhD
Level: 1-2 (Beginner - Intermediate)
Location: SnowCreek
MALDI 201
Quantitative MALDI-TOF Mass Spectrometry for Clinical Applications
Continued from Sunday
Marvin Vestal, PhD, Mark Duncan PhD, Ken Parker, PhD & Steve Hattan, PhD
Level: 2 (Intermediate)
Location: Andreas
6:00 PM
8:30 PM
OPENING RECEPTION
@ Exhibit Hall

Enjoy mingling with colleagues and Exhibitors. Take time to explore the Posters.

Appetizers and Drinks to be provided.

7:00 PM
8:00 PM
POSTERS
@ Exhibit Hall

ALL Posters Attended & Presented.
8:30 PM
10:00 PM
HOSPITALITY
@ Rocks Lounge

Enjoy the Palm Springs atmosphere on the patio overlooking the pool, with fire pits.
Drinks and appetizers provided.
10:00 PMMONDAY CLOSED

TUESDAY

6:00 AM
7:00 AM
YOGA
@ San Jacinto

Energize yourself for the day! Yoga is a complimentary offering for all MSACL registrants.
A limited number of yoga mats will be provided.
7:00 AM
8:00 AM
BREAKFAST
@ Renaissance Ballroom Foyer

Enjoy a light continental breakfast before exploring a corporate workshop.
6:00 AM
8:00 AM
PLACE POSTERS
@ Exhibit Hall

Posters must be placed by the end of breakfast.
8:00 AM
9:00 AM

CORPORATE WORKSHOPS (AM)

Bruker
Chino

(1) Advances in MALDI Imaging of Clinical Samples, (2) The Chemistry of the Human Lung Associated with Cystic Fibrosis
(1) Jeffrey Spraggins, PhD, Vanderbilt, (2) Pieter Dorrestein, PhD, University of California, San Diego

(1) In recent years, instrumentation for imaging mass spectrometry has reached unprecedented speed and sensitivity. Bruker’s rapifleX MALDI Tissuetyper, a next-generation MALDI TOF mass spectrometer was designed for imaging on clinical timescales. This presentation will review some significant advances and demonstrate applications for the imaging of clinically important tissue specimens.
(2) The microbiome is critical to human health yet we know little about the chemical environment that our microbes live in. In this presentation we will explore untargeted metabolomics strategies to reveal the chemical environment of the microbiome. In this presentation we will highlight the metabolome of a human lung associated with Cystic Fibrosis in 3D and its relationship to medicines, metabolism of medicines and microbes .

Restek
Pueblo

Selectivity Accelerated: Utilizing Raptor™ LC Columns for Clinical Solutions
Frances Carroll, Shun-Hsin Liang, Sharon Lupo

Superficially porous particles (SPP) have been proven to provide fast separations without the need for expensive instrumentation, thereby increasing sample throughput without capital investment. Although column efficiency considerably accelerates analysis time, it has little effect on resolution. Conversely, selectivity has a substantial impact on resolution, but shows minimal improvement in analysis times. By being the first to combine the speed of SPP with the resolution of Restek’s unique Ultra Selective Liquid Chromatography technology, Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast and efficient method development. This workshop will discuss how to capitalize on the selectivity of Raptor™ LC columns in challenging clinical analyses by providing application examples.

Waters
Mojave Learning Center

Towards Standardization in Protein Quantification Workflows
Paula M. Ladwig, MS, MT(ASCP); Development Technologist Coordinator with Department of Laboratory Medicine & Pathology at Mayo Clinic in Rochester MN, Mary Lame, Senior Applications Scientist, Waters Corporation

This workshop highlights the implementation and benefits of generic, kitted methods used to simplify, streamline, and standardize common protein quantification workflows, while reducing variability and delivering accurate and precise results. Data for infliximab, adalimumab, trastuzumab, bevacizumab, and the antibody drug conjugate T-DM1 will be shown as working examples. For full abstract go to: www.waters.com/msacl

8:15 AM
9:15 AM
WELCOME COFFEE
@ Renaissance Ballroom Foyer

A quick morning charge-up before the day begins.
9:15 AM
9:30 AM
WELCOME, INTRODUCTION & ORIENTATION
@ California Ballroom

Time to get informed on what's been happening at MSACL and get up to speed on logistical details that will make your life (and ours) a bit easier over the next three days. :)



Distinguished Contribution Award & Plenary Lecture
@ California Ballroom
Chair: Russell Grant & Andy Hoofnagle
9:30 AM
10:30 AM
What We Can Learn from a Drop of Urine – Metabolomics at It’s Earliest: Discoveries of Bile Acid Synthesis Disorders, a New Category of Fatal Metabolic Liver Disease and Development of a Treatment
Kenneth Setchell
Cincinnati Children’s Hospital Medical Center

Distinguished Contribution Award Lecture

This presentation will highlight how mass spectrometry was successfully applied to define new genetic defects in the cholesterol-bile acid biosynthetic pathway as a specific class of metabolic liver disease. Bile acid synthesis disorders due to single enzyme defects generally present in infancy or early childhood with a progressive cholestatic hepatitis that, unchecked, lead to cirrhosis, liver failure, and death. Prior to the seminal work of Setchell and colleagues in identifying 6 genetic diseases as discrete entities, and conceiving of an effective therapy, children with these autosomal recessive diseases either underwent liver transplantation, or more commonly, were given supportive care until they died of liver failure of unknown origin. To be described are the combined use an untargeted and targeted approach with FAB-MS, GC-MS and ESI-LC-MS/MS that led to the elucidation of the biochemical basis of these diseases, the development of an international screening program, and the evaluation of the therapeutic responses that served to ultimately gain regulatory approval from the FDA for a life-saving therapy based on oral administration of cholic acid. This application of mass spectrometry to clinical chemistry has been a game-changer that has led to a radical change in the evaluation and treatment of patients with idiopathic progressive familial intrahepatic cholestasis syndromes.1
10:30 AM
11:30 AM
COFFEE BREAK
@ Exhibit Hall

Visit the Exhibit Hall to procure coffee, juice, water and/or light snacks.

Explore what"s on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today your poster should have been up 2 hours ago. If it is not up, please put it up immediately.You will be attending your poster for 1 hour at either 3PM or 5 PM.

GENERAL SCIENTIFIC SESSION 1
Track 1
Mojave Learning Center
Introduction to Clinical Mass Spectrometry
Chair: Breland Smith
Track 2
Catalina
Metabolomics 1
Chair: Bridgit Crews
Track 3
Madera
Proteomics - Cancer Biomarkers
Chair: Andy Hoofnagle
Track 4
Pasadena
Tissue Imaging - Spatial Proteomics
Chair: Joerg Hanrieder
Track 5
Sierra
Advancements in LC-MS/MS for Small Molecule Endocrinology
Chair: Michael Chen
Track 6
SmokeTree
Application of HRMS for Toxicology Testing
Chair: Jason Sawyer
11:30 AM
11:50 AM
Overview of MS Instrumentation
Breland Smith
University of California, San Diego
Investigating Serum Metabolites as Circulating Biomarkers of Occupational Exposure to the Artificial Butter Flavoring Compound 2,3-butanedione
J. Will Thompson
Duke University
MASS-FIX: A Comprehensive Methodology for Assessment of M-proteins Using Nanobody-Enrichment Coupled to MALDI-TOF Mass Spectrometry
John Mills
Mayo Clinic
View Video
Automated Anatomical Atlas-assisted Interpretation of Differentially Expressed Proteins in Imaging Mass Spectrometry
Nico Verbeeck
Delft University of Technology
Drug Stimulated Endocrinopathy: Impact of Opiates on Free Hormone Concentrations
Julie Ray
ARUP Laboratories
New Method Validation of a Broad Spectrum Drug Screen Using a High Resolution Time-Of-Flight Mass Spectrometer in the Clinical Toxicology Laboratory
Imir Metushi
UC San Diego Health
11:50 AM
12:10 PM
Basics: Tuning Your Mass Spectrometer
Jane Yang
UCSD
Abiraterone Metabolism in Castration Resistant Prostate Cancer
Mohammad Alyamani
Cleveland State University
How to Avoid a Bone Marrow Biopsy when Monitoring Minimum Residual Disease in Multiple Myeloma: Hope for the Future!
H. Robert Bergen, III
Mayo Clinic
View Video
Matrix-free Imaging of Metabolites and Lipids in Tissues and Bacterial Colonies by LAESI and NAPA Mass Spectrometry
Akos Vertes
George Washington University
View Video
Are We Getting What We Paid for with a 13C-labeled Internal Standard?
Zlatuse Clark
ARUP Laboratories
New Library Search Algorithm Improves Identification of Drugs in Urine by LC-QqTOF
Jennifer Colby
Vanderbilt University
12:10 PM
12:30 PM
Compound Specific Tuning
Imir Metushi
UC San Diego Health
Right Target – Right Patient: Metabolomics Analysis Driving Pharmaceutical Development
Vladimir Tolstikov
BERG
Mass Spectrometry Quantification of Personalized Biomarkers for Multiple Myeloma
Melissa Hoffman
Moffitt Cancer Center/University of South Florida
View Video
Next-generation MALDI-IMS Capabilities for Ultra-high Throughput and Mass Resolution Protein Imaging
Jeffrey Spraggins
Vanderbilt University
Analysis of Aldosterone/plasma Renin Activity in a Single Method by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS)
Dennis Orton
University of Calgary
High Resolution Mass Spectrometry for Suspect Screening of Novel Psychoactive Substances: Is It a Viable Solution for Clinical Laboratories?
Kara Lynch
University of California San Francisco
12:30 PM
2:00 PM
LUNCH
@ Exhibit Hall

Basic Lunch with sandwich, chips and fruit to be provided in the Exhibit Hall.

Seating provided in Exhibit Hall, Lobby, Esplanade AND the East Lawn next to SmokeTree.

• Get ready to join a Corporate Workshop at 2:00 PM.

2:00 PM
3:00 PM

CORPORATE WORKSHOPS (PM)

Thermo Scientific
Mojave Learning Center

Alternate Sample Preparation Approaches for Your Mass Spectrometry Clinical Research Assays
William Clarke, PhD, The Johns Hopkins University School of Medicine

Pre-Register

The first part of this session will focus on the research of cell disintegrated blood (CDB) as a matrix for the analysis of immunosuppressant drugs. Opportunities, challenges, and limitations of CDB will be explored. The second part of this session will focus on alternate sample preparation approaches for the analysis of dried urine spots. Research analysis of field samples from HIV prevention trials compared to research analysis of liquid urine will be discussed, as well as other potential opportunities and limitations of the methodology.

SCIEX
Catalina

Development of High Sensitivity MicroLC-MS/MS Method for Estradiol Research in Human Serum Without Derivatization
Jerry Yeo, Ph.D, Xin Yi, Ph.D

During this presentation, we will discuss the technical challenges of developing high sensitivity LC-MS/MS methods for estradiol in blood, how to improve sensitivity and reduce ion suppression for estradiol on MicroLC-MS/MS system, and discuss the advantages and disadvantages of using microLC system versus normal flow LC system

Neoteryx
Madera

The Application & Benefits of Integrating Volumetric Absorptive Microsampling (VAMS) into the Clinical Lab : 1) Automated, Bottom-up Proteomics Workflow 2) LC-MS/MS Assay for Immunosuppressant Monitoring 3) Monitoring of Therapeutic Drug by LC-MS/MS & Diagnostic Biomarkers by ELISA
1) Irene van den Broek, PhD, Cedars-Sinai Medical Center; 2) Paul J. Jannetto, PhD, Mayo Clinic; 3) Ying Qu, PhD, Exagen Diagnostics

Pre-Register

Talk 1) discusses application of VAMS for protein biomarkers, focusing on the optimization of protein extraction from 10µL of blood & integration into a bottom-up proteomics workflow on an automated liquid handler. Talk 2) will look at feasibility of measuring Tacrolimus and Cyclosporin A with a HPLC-MS/MS assay using dried blood from a VAMS device (20µL) compared to a validated method using 200µL venous collected EDTA whole blood. Talk 3) will review a DBS comparative study (whole blood & red blood cell results) where VAMS (10µL) was applied in monitoring methotrexate polyglutamates (MTXPG1-5) in methotrexate (MTX) treated patients by a LC-MS/MS method as well as discuss how VAMS was used in ELISA based biomarker monitoring of rheumatoid factors (e.g. anti-IgM RF, anti-CCP) used as diagnostic biomarkers for autoimmunological disease.

Thomson Instrument Co
Pasadena

Streamlined Sample Preparation of Biological Samples Using the Thomson Filter Vials & Analysis by LC-MS/MS
Lisa Wanders

Sample preparation continues to be a critical factor in the quantitative measurement of biological samples. The goal of this seminar is to discuss how to streamline the sample preparation process of oral fluids, urine and blood utilizing the Thomson eXtreme, nano and the eXtractor3D Filter Vials. Reducing interferences from sample matrices and increasing analyte recovery are key requirements for preparing biological samples. Thomson Filter Vials save time, reduce solvent usage, alleviate the need for expensive consumables and lab equipment. Sample preparation for matrices such as urine, blood and oral fluids will be discussed.

SimulTOF
Sierra

New technology for imaging of biological tissues by MALDI-TOF
Stephen Hattan

This novel approach improves sensitivity, image resolution, data quality, consistency, reproducibility, flexibility, protocol simplicity and cost of analysis for MALDI-IMS. It uses chemically modified µ-channel plates to act as substrates, and simplifies experimental protocol by removing restrictions for cryogenically sliced tissue specimens.

3:00 PM
4:00 PM
COFFEE BREAK
@ Exhibit Hall

Visit the Exhibit Hall to procure coffee, juice, water and/or light snacks.

Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, check out the posters, or go for a short walk to refresh for the next session.

3:00 PM
4:00 PM
POSTERS
@ Exhibit Hall

Even-Numbered Posters to be Attended & Presented.
GENERAL SCIENTIFIC SESSION 2
Track 1
Mojave Learning Center
Best Practices in Small Molecule Data Review
Chair: Sheng-Ying (Margaret) Lo
Track 2
Catalina
Fatty Acids for Microbiology Identification
Chair: Kent Voorhees
Track 3
Madera
Protein Markers
Chair: Chris Shuford
Track 4
Pasadena
Lipid and Metabolite Imaging
Chair: Raf Van de Plas
Track 5
Sierra
Advancements in Routine Determination of Gonadal Steroids
Chair: Dan Holmes
Track 6
SmokeTree
New Approaches to Pain Medication Compliance Monitoring
Chair: Kara Lynch
4:00 PM
4:20 PM
Basic Principles of Data Review
Robert Fitzgerald
UCSD
Metal Oxide Laser Ionization (MOLI) MS for Identification of Bacteria Using Fatty Acid Profiling
Kent Voorhees
Colorado School of Mines
Longitudinal Measurement of Protein Biomarkers in Healthy Individuals and Elite Athletes
Leigh Anderson
SISCAPA Assay Technologies
View Video
Lipid Imaging for Cancer Diagnosis by Ambient Ionization Mass Spectrometry
Livia S. Eberlin
The University of Texas at Austin
View Video
A Case Study in the Development and Implementation of a Calculated Free Testosterone Assay for the Clinical Laboratory
Benjamin Beppler
TriCore Reference Laboratories
Investigation of a Large Pain Profile from Fingerstick Capillary Blood
Jeffrey Enders
Ameritox, Ltd.
4:20 PM
4:40 PM
Data Review Using Vendor Software and Rules Systems
Krista Pratico
UCSD Center for Advanced Laboratory Medicine
Strain-level Bacterial Identification by CeO2-catalyzed MALDI-TOF MS Fatty Acid Analysis and Comparison to Commercial Protein-based Methods
Chris Cox
Colorado School of Mines
Quantitation of Albumin and Creatinine in Urine by MALDI-TOF Mass Spectrometry
Stephen Hattan
SimulTof Systems
View Video
Imaging Mass Spectrometry and Metabolomics in Parkinson’s Disease
Timothy Garrett
University of Florida
View Video
Evaluation of Matrices for the Development of Calibrators in Therapeutic Monitoring of Hormones, Drugs and Biomarkers: Testosterone Case Study
Uma Sreenivasan
Cerilliant Coproration
Specifically Opioids: The Importance of LC in LC-MS/MS in Opioid Therapy Monitoring and Compliance Testing
Matt Salske
Essential Testing
4:40 PM
5:00 PM
Automated Data Review Using Homebrew Software
Sheng-Ying Lo
University of Washington
Using Rapid Evaporative Ionisation Mass Spectrometry (REIMS) to Assign Taxonomic Classifications to Microbial Isolates and Mixed Communities
Simon Cameron
Imperial College London
Apolipoprotein C-III Proteoforms as Biomarkers for Changes in Lipid Metabolism
Olgica Trenchevska
Arizona State University
View Video
IR-MALDESI: An Innovative Approach to Molecular Microscopy
David Muddiman
North Carolina State University
View Video
Simultaneous Measurement of Testosterone and Estradiol in Serum by LC-MS/MS without Derivatization
Dean Carlow
Memorial Sloan Kettering Cancer Center
Parent Drug - Metabolite Relationship of Opiates in Oral Fluid: Implications of the 2015 SAMHSA Guidelines
Joseph Rudolf
Massachusetts General Hospital
View Slides (PDF)
5:00 PM
7:00 PM
RECEPTION
@ Exhibit Hall

Enjoy mingling with colleagues and Exhibitors. Take time to explore the Posters.

Appetizers and Drinks to be provided.

5:00 PM
6:00 PM
POSTERS
@ Exhibit Hall

Odd-Numbered Posters to be Attended & Presented.

All posters to be removed by 7:30 PM.

7:00 PM
8:00 PM

DISCUSSION GROUPS
@ Rooms around Ballroom Foyer

Exhibitor Feedback
@ Andreas
Lead(s): David Herold & Chris Herold

Exhibitors, let MSACL know your thoughts on the way the conference is working for you and what we can do to make the experience better in the long run.

CDC Standardization Programs Forum
@ Chino
Lead(s): Hubert Vesper & Julianne Botelho

Participants will discuss how CDC Standardization Programs support laboratories with improving measurements for key hormones such as 25-hydroxyvitamin D, estradiol, and testosterone. Included will be additional discussions about new projects and tools available in CDC Standardization Programs.

Convincing Administrators (and the Public) of the Benefits of MS in the Clinic
@ Pueblo
Lead(s): Soumen Manna

Assemble highlights for the purpose of creating compelling powerpoint for those new to the field (i.e. clinical lab directors, managers, public). Discuss MS-based methods with respect to their technical and economic superiority/inferiority to the existing/emerging alternatives. Identify examples (if any) existing or emerging diagnostic/prognostic tests where MS is the only viable option. Highlight green aspects comparing environmental footprints (energy, toxic waste, chemical and bio-hazards) vis-a-vis existing techniques. Review evolution and adoption of Clinical MS, explore challenges, ethics, mistakes.

MSACL Fundamentals Track - What's Next?
@ Mojave Learning Center
Lead(s): Robert Fitzgerald & Judy Stone

Feedback on the Fundamentals / Newbies track. How is it going? What can be improved?

Understanding Patents in Mass Spectrometry
@ Catalina
Lead(s): Ben Borson

This discussion group will review real world patent claims in the field of metabolite analysis using mass spectrometry and some simpler ‘made-up’ claims to help scientists understand what activities would fall under the experimental use doctrine, direct infringement, indirect infringement, contributory infringement, infringement under the doctrine of equivalents and how to design around infringement of a method or a device claim.

CSI - MSACL
@ Madera
Lead(s): Robert Kobelski & Jack Henion

A fun-filled opportunity to apply mass spectrometry tools and talents to solve a case of mysterious exposure to chemicals of medical significance. Crime scene data can include GC-MS results and olfactory clues. Clinical analysis results can include; clinical presentation, symptoms, GC-MS, ICP-MS and/or LC-MS data. This is an opportunity to flaunt you MS interpretation skills in front of your peers.

Eating A Whale, One Bite At A Time
@ Pasadena
Lead(s): Russell Grant & Brian Rappold

Yo ho ho mateys’ - it’s time to charge the guns and hoist the mainsail. The evenings’ entertainment will focus on bottom up protein assays through finding the flaws in proposed methods. Let the grog flow and battle(ships) commence as we learn how to eat a whale of a “clinical protein” assay.

Early Career Development Council
@ Sierra
Lead(s): Jane Yang

To create the opportunity for clinical mass spectrometrists, early in their careers, to assemble with the intent of creating and maintaining an interest group with a discernible voice that will communicate directly and effectively with MSACL, MSACL vendors and the clinical mass spectrometry community at large.

8:00 PM
10:00 PM
HOSPITALITY
@ Rocks Lounge

Enjoy the Palm Springs atmosphere on the patio overlooking the pool, with fire pits.
Drinks and appetizers provided.
10:00 PMTUESDAY CLOSED

WEDNESDAY

6:00 AM
7:00 AM
YOGA
@ San Jacinto

Energize yourself for the day! Yoga is a complimentary offering for all MSACL registrants.
A limited number of yoga mats will be provided.
7:00 AM
8:00 AM
BREAKFAST
@ Renaissance Ballroom Foyer

Enjoy a light continental breakfast before exploring a corporate workshop.
6:00 AM
8:00 AM
PLACE POSTERS
@ Exhibit Hall

Posters must be placed by the end of breakfast.
8:00 AM
9:00 AM

CORPORATE WORKSHOPS (AM)

SimulTOF
Chino

Quantitative MALDI-TOF mass spectrometry for clinical applications
Marvin L. Vestal PhD

MALDI-TOF MS is used for analyzing a variety of nonvolatile molecules, but acceptance has been limited by the belief that MALDI-TOF is not quantitative. Results are presented on new MALDI-TOF mass spectrometers that generate reproducible spectra on complex samples. High sensitivity and accurate masses are achieved by operating at laser rates up to 5 kHz and summing up to 500,000 shots/sample. Recent and potential clinical applications are reviewed.

SCIEX
Pueblo

The Lipidyzer: The Convergence of Differential Ion Mobility and Mass Spectrometry to the Next Generation Lipidomics Analysis Platform
Paul Baker, Ph.D

There is growing interest in characterizing lipid molecular species as clinical biomarkers of human disease. MS-based lipidomics data arrays are processed by lipid ID software followed by PCA to generate candidate lipid biomarkers. This process is challenging due to the numerous lipid isobars that interfere with quantitation. Differential Mobility Spectrometry (SelexION® Technology) coupled to MS analysis is effective at resolving complex lipid mixtures. This technology has been applied to quantitative lipidomics using the LipidyzerTM, which detects and measures over 1200 lipids with absolute quantitation. In this presentation, the application of SelexION® Technology to global and targeted lipidomics will be discussed, and data will be shown demonstrating how it has fundamentally changed the future of lipid analysis.

Thermo Scientific
Mojave Learning Center

Which Chromatography and Mass Analyzer is Right for Your Clinical Research Application?
Lewis Couchman, Kings College Hospital

Pre-Register

Mass spectrometry has wide applicability in clinical research, especially when combined with modern chromatography and sample preparation. However, clinical research method development should remain application-centered, and ideally use a range of approaches to design a ‘fit-for-purpose’ methodology.We will describe the applicability of turbulent flow chromatography, high-resolution, accurate mass (HRAM) LC-MS, and rapid quantitative analysis using triple quad mass analyzers. The merits of each will be discussed using examples from clinical research methods.

Plenary Lecture Series
@ California Ballroom
Chair: Daniel Holmes
9:15 AM
10:00 AM
Diagnosis - The Beauty and the Beast
Mark Graber
Society to Improve Diagnosis in Medicine
View Video
Diagnosis is perhaps the most complicated cognitive task humans face. Despite the many challenges involved, the correct diagnosis is established in the great majority of cases, thanks in very large part to advances in medical testing, such as mass spectrometry. At the same time, diagnostic errors are too common and cause enormous harm. The origins of diagnostic errors will be explored, providing insights that can improve not only diagnosis, but the decisions we make in our everyday lives and research enterprises.
10:00 AM
10:45 AM
Immunotherapy of Cancer and the Role of Mass Spectromety; an Overview
Donald Hunt
University of Virgina
View Video
This lecture will describe how the immune system works to keep us free of cancer most of the time and how cancer cells can eventually learn to escape the immune system. Also described will be a number of scientific breakthroughs that have occurred in the last 6 years that clearly suggest that the immune system can be re-educated and upregulated to cure even late stage cancer. Science magazine labeled cancer immunotherapy as the breakthrough event of the year in 2013 and tremendous progress has been made since then. James Allison won the 2015 Lasker Award in medicine for his work to reactivate the immune system to fight cancer. You know this is a hot area for mass spectrometry when the research groups of Aebersold, Heck, Mann and Carr all decide to establish and work on the Human Immuno-Peptidome Project (HIPP) at the HUPO 2015 meeting in Vancouver. This lecture will try to capture some of this excitement and also pinpoint some of the contributions that the Hunt group has made to the field over the past 23 yrs.
10:45 AM
11:30 AM
COFFEE BREAK
@ Exhibit Hall

Visit the Exhibit Hall to procure coffee, juice, water and/or light snacks.

Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk to refresh for the next session.

POSTER PRESENTERS: If you are presenting a poster today your poster should have been up 2 hours ago. If it is not up, please put it up immediately. You will be attending your poster for 1 hour at either 3PM or 5 PM.

GENERAL SCIENTIFIC SESSION 3
Track 1
Mojave Learning Center
Sleep Soundly at Night when Using LC-MSMS - Quality & Metrics
Chair: Lori Bachmann
Track 2
Catalina
Metabolomics 2
Chair: Michael Gelb
Track 3
Madera
Proteomic - Advances in the Clinic
Chair: Surendra Dasari
Track 4
Pasadena
Bioinformatics for Clinical MS Imaging
Chair: Tim Garrett
Track 5
Sierra
Computational Aids to Assay Development in LC-MS/MS
Chair: Steve Master
Track 6
SmokeTree
New Sample Types and Introduction Techniques for Drug Analysis
Chair: Russell Grant
11:30 AM
11:50 PM
A Regulatory Review of Quality Assurance Monitoring for LC-MS
Kara Lynch
University of California San Francisco
Tandem Mass Spectrometry for Newborn Screening and Diagnosis of Lysosomal Storage Diseases
Michael Gelb
Univ. of Washington
Mass Spectrometry Analysis of CNS Specific Oligoclonal Immunoglobulins
Patrick Vanderboom
Mayo Clinic
View Video
Integrating Mass Spectrometry with Other Imaging Technologies: Improving Biological Insight Through Multi-modal Image Fusion
Raf Van de Plas
Delft University of Technology
Application of MUSCLE Software as an Automated Approach to LC-MS/MS Method Development for the Analysis of Multiple Vitamin D Metabolites
Carl Jenkinson
Metabolism and Systems Research, University of Birmingham
Analysis of 29 Drugs of Abuse in Exhaled Breath Using UHPLC-MS/MS
Shahid Ullah
Karolinska Institute
11:50 AM
12:10 PM
Part 1: Effective Utilization of Quality Assurance Metrics in LC-MS/MS: Practical Examples
Lorin Bachmann
Virginia Commonwealth University
Examination of S-adenosylmethionine/S-adenosylhomocysteine and DNA Methylation in Mouse Models of Cystathionine β-synthase Deficiency
Yin-Ming Kuo
Fox Chase Cancer Center
Resolving Discrepancies in Immunonephelometric Total IgG and IgG Subclass Measurements with Mass Spectrometry
Andre Mattman
University of British Columbia
View Video
Statistical Methods for Mass Spectrometry-based Imaging
Olga Vitek
Northeastern University
View Video
Alternative Approaches for Producing Micro Samples of Dried Plasma for Automated LC/MS/MS Determination of Opioid Drugs
Jack Henion
Q2 Solutions
Advances in the Development of Touch Spray Mass Spectrometry with Medical Swabs for Drug Detection in Oral Fluid
Valentina Pirro
Purdue University
12:10 PM
12:30 PM
Part 2: Effective Utilization of Quality Assurance Metrics in LC-MS/MS: Practical Examples
Lorin Bachmann
Virginia Commonwealth University
Decompensation Events in Maple Syrup Urine Disease (MSUD): The Impact of Mass Spectrometry on Acute Management
Andy De Souza
BC Children's Hospital
Peptide Selection for Amyloidosis Diagnosis and Typing
Han-Yin Yang
University of Washington
View Video
De novo Discovery of Phenotypic Intra-tumor Heterogeneity Using Imaging Mass Spectrometry
Benjamin Balluff
Maastricht University
Design of Experiments for Optimization of Quantitative LC-MS/MS Clinical Diagnostic Methods
Margret Thorsteinsdottir
University of Iceland
Rapid Characterization of Drugs and Metabolites on Skin by Ambient Mass Spectrometry
Jentaie Shiea
National Sun Yat-Sen University
12:30 PM
2:00 PM
LUNCH
@ Exhibit Hall

Basic Lunch with sandwich, chips and fruit to be provided in the Exhibit Hall.

Seating provided in Exhibit Hall, Lobby, Esplanade AND the East Lawn next to SmokeTree.

• Get ready to join a Corporate Workshop at 2:00 PM.

2:00 PM
3:00 PM

CORPORATE WORKSHOPS (PM)

Waters
Mojave Learning Center

The Impact of Evolving LC-MS/MS Technologies upon Clinical Research Methods
Tim Wood, Biochemical Genetics Laboratory, Sarah Young, Duke Hospital Biochemical Genetics Lab

As the performance of all aspects of LC-MS/MS technology has progressed over the years, clinical research methods have benefited from increasing analytical sensitivity, specificity and throughput. In turn, this has lead to the sharpening of approaches for the detection of disease-specific biomarkers, and ultimately a better understanding of the underlying biochemistry for a number of metabolic disorders. In this workshop, you will hear experiences from two prominent experts in the biochemical genetics community who will describe the effects of evolving LC-MS/MS technology upon their research. For full abstract go to: www.waters.com/msacl

Phenomenex
Catalina

1. Spitting in the Face of your MS, and Why That is a Bad Idea. 2. Automation in the LCMS Laboratory
1. Sean Orlowicz, Seyed Sadjadi - Phenomenex 2. Rohit Shroff - Tecan

1. Oral Fluids provide many advantages over more invasive sample collection options, however their LCMS analysis can come with litany of new challenges. In this talk, we focus on overcoming some of these challenges including; sensitivity, matrix effects and the recovery of multiple drug classes, through the use of sample preparation and HPLC techniques. 2. Increasing test volumes of mass spectrometry assays in the clinical laboratories are challenging many to revisit the major bottleneck in their workflows – manual sample preparation. Tecan has developed tailored workflow automation solutions for a variety of clinically important analytes and extraction methodologies to alleviate this bottleneck. In this talk, we will investigate some of the tools available to improve your workflow.

Biotage
Madera

Small Sample Volumes Are Small Problems
Kris Franklin, Lite Consulting

With advances in LC-MS/MS technology and automated sample preparation, the need for large sample volumes are no longer a requirement for many assays. The sensitivity and specificity of modern LC-MS/MS allows for multi-analyte panels to be targeted, which has the potential to reduce cost for clinical lab operation. The challenge of multiplexing targets into a single LC-MS/MS run is the development of a robust sample prep that sufficiently removes sample matrix and interfering substances or buffers that may harm or reduce sensitivity of the instrument, while maintaining adequate recovery of the targets. Recent developments in our lab have allowed for reduced sample volumes while producing clinically relevant values for both toxicology and endocrinology. This seminar will include several application examples including oral fluids, urine and hormone quantification in whole blood.

Agilent Technologies
Pasadena

MS in the Clinical Laboratory: Roles, Challenges, Processes, and the Future
Paul J. Jannetto, Ph.D., DABCC, FACB, M.T.(ASCP), Consultant, Mayo Clinic, Department of Laboratory Medicine and Pathology, Rochester, MN

Pre-Register

This talk will explore the increasing role of Mass Spectrometry in clinical laboratories; focusing on the challenges (economic, regulatory, and validation) and how these challenges may be addressed using the Mayo test life cycle. We will also get a glimpse into future applications and full laboratory automation.

Sigma-Aldrich
Sierra

Managing Matrix Impact: High -throughput 25-hydroxy Vitamin D Testing with LC-MS/MS with Phospholipid Depletion
Judy Stone and Craig Aurand

Craig Aurand will cover the impact of phospholipid matrix interference and describe techniques to minimize the impact on LC/MS assays. Judy Stone will describe the development of a high-throughput 25-hydroxy Vitamin D (VITD) clinical assay at the Northern California Kaiser Regional Laboratories. In 2010 their test volume for serum increased 30-40% per year, and by 2011, the lab anticipated receiving 1200-1500 samples a day for this test. Stone will describe the rationale and methodology for use of Hybrid-SPE plates for VITD sample prep to add the analytical and process robustness that was considered critical for such an ambitious change in workflow to succeed. By 2015 the lab was testing for VITD at the max LC-MSMS throughput of 2200 samples a day with this protocol, maintaining excellent performance metrics for accuracy and precision.

3:00 PM
4:00 PM
COFFEE BREAK
@ Exhibit Hall

Visit the Exhibit Hall to procure coffee, juice, water and/or light snacks.

Explore what's on offer from the Exhibiting vendors, reconnect with colleagues, or go for a short walk to refresh for the next session.

3:00 PM
4:00 PM
POSTERS
@ Exhibit Hall

Even-Numbered Posters to be Attended & Presented.
GENERAL SCIENTIFIC SESSION 4
Track 1
Mojave Learning Center
Sleep Soundly at Night when Using LC-MSMS - Quality & Personnel
Chair: Shannon Haymond
Track 2
Catalina
Microbial Analysis
Chair: Nate Ledeboer
Track 3
Madera
Proteomics - Optimizing Quantitative Methods
Chair: Irene Van Den Broek
Track 4
Pasadena
High Spatial Resolution Imaging
Chair: Jeff Spraggins
Track 5
Sierra
HRMS: From OR to the Surgical Path Lab
Chair: Brian Rappold
Track 6
SmokeTree
MS Small Molecule Analysis
Chair: Mark Marzinke
4:00 PM
4:20 PM
Setting Up Your Mass Spectrometry (MS) Team for Success
Daniel Holmes
University of British Columbia
View Slides (PDF)
Comprehensive Metaproteomic Analyses of Urine in Cases of Urinary Tract Infection and Colonization/Absence of Bacteriuria
Yanbao Yu
J. Craig Venter Institute
Using a Stable Isotope-labeled Protein and Corresponding Set of Stable Isotope-labeled Peptides as Comprehensive Quality Controls for Bottom-up Proteomics
James Bollinger
University of Washington
View Video
Cell-by-cell Measurement of Metabolic Activity in the Early Developing Embryo
Peter Nemes
George Washington University
Emerging Methods for Orthogonal Workflow Compatible Automated and Semi-Automated Cytological, Histological, and Analytical Sample Evaluation
Mariam Elnaggar
Prosolia
Effective Strategies for Overcoming Polyatomic Spectral Interferences During the Measurement of Trace Metals in Whole Blood Samples by ICP-MS
Brooke Katzman
Mayo Clinic
4:20 PM
4:40 PM
Part 1: Tips and Tools for Training and Competency with Clinical LC-MS
Shannon Haymond
Northwestern University FSM
Identification of Bacteria by MALDI by Matching to Translated DNA Databases
Kenneth Parker
SimulTOF Systems
Is It Better to Be Lucky or Absolute in Protein Quantification?
Christopher Shuford
Laboratory Corporation of America
Molecular Diagnosis of Benign and Malignant Melanocytic Lesions Using Mass Spectrometry Imaging
Erin H Seeley
Protea Biosciences, Inc.
View Video
Why the Operating Room Needs Mass Spectrometry (And Doesn’t Even Know It)
Alyssa Burgart
Stanford University
Quantification of 15N-nitrite and 15N-nitrate in Human Biological Matrices: An LC-MS/MS Application to Study the Development of Nitrate Tolerance
Elizabeth Axton
Oregon State University
4:40 PM
5:00 PM
Part 2: Tips and Tools for Training and Competency with Clinical LC-MS
Shannon Haymond
Northwestern University FSM
Targeted Metabolic Profiling for Qualitative and Quantitative Measurement of Bacterial Metabolites and their Response to Antibiotic Treatment
Jiangjiang (Chris) Zhu
Miami University
Strategies for Improving Specificity and Sensitivity for Multiplexed Proteomic Assays in for High-Flow, Fast-Gradient LC-MS in the Clinical Laboratory
Timothy Collier
Cleveland HeartLab Inc
View Video
High Resolution Molecular Imaging: Revealing More Detail in Clinical Studies
Ron M.A. Heeren
Maastricht University, M4I
View Video
Real-time Classification of Ex-vivo Breast Tissues by Rapid Evaporative Ionization Mass Spectrometry with a Combination of Electrosurgical Modalities
Edward St John
Imperial College
Taking the Hit Out of HIT: Heparin Induced Thrombocytopenia Assessment via LC-MS/MS Analysis of Endogenous Serotonin Release
Matthew Crawford
Lab Corp
5:00 PM
7:00 PM
RECEPTION
@ Exhibit Hall

Enjoy mingling with colleagues and Exhibitors. Take time to explore the Posters.

Appetizers and Drinks to be provided.

5:00 PM
6:00 PM
POSTERS
@ Exhibit Hall

Odd-Numbered Posters to be Attended & Presented.

All Posters should be removed by 7:30 PM.

7:00 PM
8:00 PM

CORPORATE WORKSHOPS (Evening)

ZefSci
Mojave Learning Center

(1) Project Management for Method Validation (2) Simplifying Method Validation; Benefits of a Kitted Solution
(1) James Byrd, Project Manager & Application Chemist, Zef Scientific, Inc. (2) François Espourteille, Founder, Comprehensive LCMS Solutions

Pre-Register

(1) Preparing for a full method validation can be a daunting task. Join us to see how the tools & techniques of Project Management can make the process more predictable, more efficient, and more flexible. Example forms will be provided to the first 40 registrants. (2) Installation and validation of an assay is a complex process. We will discuss how incorporating a kitted solution removes some of the hurdles of setting up and validating an assay.

8:00 PM
10:00 PM
HOSPITALITY
@ Rocks Lounge

Enjoy the Palm Springs atmosphere on the patio overlooking the pool, with fire pits.
Drinks and appetizers provided.
10:00 PMWEDNESDAY CLOSED

THURSDAY

6:00 AM
7:00 AM
YOGA
@ San Jacinto

Energize yourself for the day! Yoga is a complimentary offering for all MSACL registrants.
A limited number of yoga mats will be provided.
7:00 AM
8:00 AM
BREAKFAST
@ Renaissance Ballroom Foyer

Enjoy a light continental breakfast before exploring a corporate workshop.
8:00 AM
9:00 AM

CORPORATE WORKSHOPS (AM)

EMD Millipore
Chino

#1 - Preparing Clinical Samples for Meaningful Mass Spectrometry (MS) Up to 75% of the work and expenditure of an analytical lab is spent on sample preparation.
Ivona Strug

Choosing the right sample prep methods tools can improve the MS workflow and save time. Attend our popular workshop on contaminants and matrix components that potentially interfere with MS analysis, and how you can minimize interference with proper sample preparation. Some of the most expensive errors in MS analyses are caused by sample particulates. Particulates are usually removed by filtration, which could introduce further contamination if incompatible or low-quality filter devices are used. Our presentation will focus on practical solutions for sample and solvent filtration--how to choose the right filter and avoid leached contaminants. You will learn tips for simple, fast sample preparation using centrifugal devices (FASP, or filter-aided sample prep). We will also show how to make your processed samples more LC-MS-ready by applying ZipTip® devices.

Hamilton
Mojave Learning Center

: Hamilton Robotics: Innovative High-throughput Sample Preparation Modules for Bioanalytical Analysis
Michael Mouradian, Ph.D. Scientific Leader, Drug Discovery, Hamilton Robotics

This workshop will discuss Hamilton’s automation tools for bioanalytical workflows focusing on clinical laboratories. Workshop highlights will include, new modules for automated mass spectrometry sample preparation and robust sample preparation methods for reliable, high-throughput analysis.

Shimadzu
SmokeTree

Pharmacogenomics- and Therapeutic Drug Monitoring- Guided Treatment for Precision Patient Care.
Kevin Rosenblatt, MD, PhD, CMO, CSO & Lab Director, CompanionDx Labs

This interactive workshop will take a closer look at the use of genetics, metabobolomics and proteomics measurements to suggest more effective patient therapies. Successful integration and reporting of pharmacogenomics and metabolite/'omics data, as well as the development of new MS-based technologies that enable these measurements, will be presented. Advances in ultra-fast single reaction monitoring/multiple reaction monitoring (SRM/MRM), plasma separation cards (noviplex cards, Novilytic Labs) and fully-automated clinical lab automation module (CLAM-2000) technology will be discussed. "Kick Some Mass" t-shirts will be given to the first 70 attendees!

8:15 AM
9:15 AM
WELCOME COFFEE
@ Renaissance Ballroom Foyer

A quick morning charge-up before the day begins.
Plenary Lecture Series
@ California Ballroom
Chair: Livia S. Eberlin
9:15 AM
10:00 AM
Mass Spectrometry in Clinical Science
R Graham Cooks
Purdue University
This presentation attempts to elucidate the forces converging to create a major increase in the applications (and applicability) of mass spectrometry in the clinic. The forces include: i) ambient ionization methods for direct examination of biological samples, including tissue and whole biofluids, ii) miniature mass spectrometers capable of unit resolution and MS/MS, iii) ion transfer over long distances to the Mini MS iv) quantitation out of complex mixtures by multiple reaction monitoring (MRM), and v) multivariate data analysis for confident assignment of complex spectral patterns to particular biological and disease states. Example applications include surgical margins, drug and microorganism (e.g. strep) detection.
10:00 AM
10:30 AM
POSTER AWARDS
@ California Ballroom
Chair: Jane Yang
Join us in congratulating the winners of the inaugural poster contest.
10:30 AM
11:00 AM
COFFEE BREAK
@ Renaissance Ballroom Foyer

Quick break as we move to the next scientific session.
GENERAL SCIENTIFIC SESSION 5
Track 1
Mojave Learning Center
Proteomics for the Beginner
Chair: Tim Collier
Track 2
Catalina
Chasing Ebola
Chair: Susan Butler-Wu
Track 3
Madera
Proteomics - Building Better Methods
Chair: Cory Bystrom
Track 4
Pasadena
Drug Discovery Imaging
Chair: Peter Nemes
Track 5
Sierra
Advancements in Sample Preparation for Protein Quantitation by MS
Chair: Mari DeMarco
Track 6
SmokeTree
MS Methods for 'Anti-Drugs'
Chair: Jennifer Colby
11:00 AM
11:20 PM
Part 1: Introduction to Clinical Proteomics
Timothy Collier
Cleveland HeartLab Inc
Development of a Mass Spectrometry Method for Quantifying Glycoprotein in Ebola Virus-Like-Particles
Michael Ward
USAMRIID
Gingerbread Men: A Cookie-cutter Bottom-up Proteomics Workflow for the Hungry?
Andy Hoofnagle
University of Washington
View Video
An Accurate Mass Imaging MS Approach to Support Preclinical PK/PD Assessments
Sheerin Shahidi-Latham
Genentech, Inc.
View Video
A Controlled Flow 96 Well Plate for Automation of Antibody Capture and Protein Digestion with Quantitative Determination of Surrogate Peptides by LCMSMS
John Laycock
SPEware Corporation
Development and Validation of an LC-MS/MS Sulfonylurea Assay for Hypoglycemia Cases in the Emergency Department
He Yang
UCSF General Hospital
11:20 AM
11:40 PM
Part 2: Introduction to Clinical Proteomics
Timothy Collier
Cleveland HeartLab Inc
Detection and Discovery of Early Markers of Ebola Virus Infection Using Serum Proteomic Analysis
Lisa Cazares
USAMRIID
Design of a LC-MS/MS Plasma Protein Assay Using Data Independent Acquisition
Jarrett Egertson
University of Washington
View Video
Mass Spectrometric Innovations for Tissue Imaging and Direct Analysis
Richard Yost
University of Florida
View Video
Accelerating Mass Spectral Analysis: The Sample Preparation Component
Fred Regnier
Purdue University
Real-time PK Measurement of the Chemotherapeutic Drug Melphalan in Whole Blood by a Novel PaperSpray-tandem Mass Spectrometry (PS-MS/MS)
Junfang Zhao
Cincinnati Children’s Hospital Medical Center
11:40 AM
12:00 PM
Part 3: Introduction to Clinical Proteomics
Timothy Collier
Cleveland HeartLab Inc
Bloodborne Pathogen Contamination in the Era of Laboratory Automation and Ebola
Andrew Bryan
University of Washington
An Empirical Approach to Signature Peptide Choice for Selected Reaction Monitoring: Quantification of Uromodulin in Urine
Jennifer Van Eyk
Cedars Sinai Medical Center
View Video
Advancing Drug Development and Understanding Using Advanced Mass Spectrometry
Jeremy Norris
Vanderbilt University School of Medicine
Getting the Biggest Bang for the Buck: Manipulating Trypsin Digestion Conditions to Accelerate Digestion and Improve Signal Intensity
Yu Zi (Emma) Zheng
St.Paul's Hospital, University of British Columbia
Development and Validation of Anti-TB Drugs in Plasma to Support Pharmacokinetic-Driven Studies
Mark Marzinke
Johns Hopkins University School of Medicine
12:00 PM
1:00 PM
LUNCH
@ Renaissance Ballroom Foyer

Box Lunch to be provided in the Renaissance Ballroom Foyer.

Enjoy patio seating outside overlooking the pool.

GENERAL SCIENTIFIC SESSION 6
Track 1
Mojave Learning Center
Microbiology by MALDI: Case Studies
Chair: Lori Bourassa
Track 2
Catalina
Metabolomics 3
Chair: Rick Yost
Track 3
Madera
Proteomics - Data Acquisition for Discovery
Chair: J. Will Thompson
Track 4
Pasadena
Imaging MS in Clinical Pathology
Chair: Ron Heeren
Track 5
Sierra
Mind your P's and Q's: Processing and Quality
Chair: Shannon Haymond
Track 6
SmokeTree
Expanding Horizons in Disease Analysis
Chair: Donald Hunt
1:00 PM
1:20 PM
Part 1: MALDI-TOF Mass Spectrometry in the Clinical Microbiology Laboratory
Lori Bourassa
University of Washington Medical Center
Successes and Challenges of Metabolic Reprogramming Elucidation in Cancers Directly from Human Subjects via Stable Isotope Resolved Metabolomics (SIRM)
Richard Higashi
University of Kentucky
Scaling Discovery Proteomics to Large Lung Cancer Cohorts Using Data Independent Acquisition
John Koomen
Moffitt Cancer Center
View Video
Desorption Electrospray Ionization – Mass Spectrometry for Intraoperative Analysis of Brain Cancer Tissue
Alan Jarmusch
Purdue University
View Video
Don’t Manually Transcribe Your Results: The Poor Person’s Guide to LC-MS/MS LIS Interfacing with R
Daniel Holmes
University of British Columbia
View Slides (PDF)
Using Mass Spectrometry to Understand Cystic Fibrosis as a Protein Misfolding Disease
John Yates
The Scripps Research Institute
1:20 PM
1:40 PM
Part 2: MALDI-TOF Mass Spectrometry in the Clinical Microbiology Laboratory
Melanie Yarbrough
Washington University in St. Louis
Combining Microfluidic Separations with High Pressure Mass Spectrometry for Clinical Diagnostic Applications
J. Scott Mellors
908 Devices Inc. and University of North Carolina
Analytical Characterization of Multiplexed Parallel Reaction Monitoring Assays to Quantify N-linked Glycosite-containing Peptides in Serum
Stefani Thomas
Johns Hopkins University
View Video
Multimodal Imaging Mass Spectrometry for Probing Aβ-Plaque Pathology in Transgenic Alzheimer’s Disease Mice
Jörg Hanrieder
University of Gothenburg
View Video
Clinical Quality Control for Multiplex Mass Spectrometry
Stephen Master
Weill Cornell Medicine
Dried Blood Spot Screening for Wilson Disease Using Immuno-SRM
Sunhee Jung
Seattle Children
1:40 PM
2:00 PM
COFFEE BREAK
@ Renaissance Ballroom Foyer

Quick break as we move to the next scientific session.
GENERAL SCIENTIFIC SESSION 7
Track 1
Mojave Learning Center

Chair: TBA
Track 2
Catalina
Metabolomics Meets Alzheimer's
Chair: Charity Aiken
Track 3
Madera
Isoforms in Protein Analysis
Chair: Tim Collier
Track 4
Pasadena
Surgical Mass Spectrometry
Chair: Livia S. Eberlin
Track 5
Sierra
Protein MS: Thoughts for the Future
Chair: David Herold
Track 6
SmokeTree

Chair: TBA
2:00 PM
2:20 PM
Track ClosedAn Unbiased Investigation of the Lipidomic and Metabolomic Pathways Altered in Alzheimer’s Disease Brain
Giuseppe Astarita
Georgetown University
Analysis of ‘Total’ Vitamin D Binding Protein and Albumin with Simultaneous Identification of VDBP Isoforms Using Rapid Trypsin Digestion and TurboFlow LC-MS
Lewis Couchman
King's College Hospital
Translating DESI-MSI to Clinical Pathology – Adventures and Challenges
Zoltan Takats
Imperial College London
View Video
Talk extended to 40 minutes
The Future of Mass Spectrometry-based Protein Lab Tests
Dobrin Nedelkov
Arizona State University
Track Closed
2:20 PM
2:40 PM
Track ClosedPotential of Metabolome Analysis for Early Diagnosis of Alzheimer’s Disease
Therese Koal
Biocrates Life Sciences AG
Differential MS and Targeted MS/MS Used to Identify an Aberrant ACTH Isoform
Mari DeMarco
Univ. of British Columbia, St Paul's Hospital
Mass Spectrometry for Image Guided Neurosurgery and Drug Development
Nathalie Agar
Brigham and Women's Hospital, Harvard Medical School

Cancelled
MALDI TOF Mass Spectrometry as a Tool for in Vitro Testing
Mark Duncan
School of Medicine, University of Colorado
Track Closed
2:40 PM
3:00 PM
COFFEE BREAK
@ Renaissance Ballroom Foyer

Quick break as we move to the next scientific session.
GENERAL SCIENTIFIC SESSION 8
Track 1
Mojave Learning Center

Chair: TBA
Track 2
Catalina
The Microbiome Metabolome Interface
Chair: Karen Phinney
Track 3
Madera
Innovative Topdown Protein Analysis
Chair: John Yates
Track 4
Pasadena
Molecular Histology by MALDI Imaging
Chair: Jeffrey Spraggins
Track 5
Sierra
You did that with Parafilm?
Chair: Jentaie Shiea
Track 6
SmokeTree

Chair: TBA
3:00 PM
3:40 PM
Track Closed3D Cartography of the Microbiome of the Human Skin and Lungs in Cystic Fibrosis
Pieter Dorrestein
UCSD
Innovative Instrumentation and Methods for the Identification of Intact Proteins in Mixtures and for Sequence Analysis of Antibodies and Posttranslationally-Modified, Intact Proteins on a Chromatograpic Time-Scale
Donald Hunt
University of Virgina
View Video
Imaging Mass Spectrometry: Molecular Microscopy for Biology and Medicine
Richard Caprioli
Vanderbilt University
Development and Applications of Sorbent-attached Membrane Funnel Spray Mass Spectrometry for Analysis of Biological Samples
Tak Wah Dominic Chan
The Chinese University of Hong Kong

Cancelled
Track Closed
3:40 PM
7:00 PM
CLOSING HOSPITALITY RECEPTION
@ Rocks Lounge

Enjoy the Palm Springs atmosphere on the patio overlooking the pool, with fire pits.
Drinks and appetizers provided.
7:00 PMCONFERENCE CLOSED