MSACL 2017 EU: Preliminary Conference Program

Salzburg, AUSTRIA • September 10 - 14, 2017

With Thanks to Our Corporate Conference Sponsors:
Thermo
SCIEX
Shimadzu  Agilent 
Chromsystems

Sunday

11:00
19:00
BADGE PICKUP
@ Registration Foyer
11:00
14:00
LUNCH ON OWN
@ Salzburg Old City

Enjoy Lunch in the Old Town of Salzburg.
14:00
18:00

SHORT COURSES
Coffee Breaks every 0:50 for 10 minutes.

COURSE START
Data Science 101
Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Stephen Master, MD, PhD
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 5
COURSE START
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 4
COURSE START
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
Erik J. Soderblom, PhD & Chris Shuford, PhD
Level: 2 (Intermediate)
Location: Paracelsus
COURSE START
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Papageno
COURSE START
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: 1-2 (Beginner to Intermediate)
Location: Doppler
COURSE START
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: 2-3 (Intermediate - Advanced)
Location: Trakl
Every 0:50
for 10 min
COFFEE BREAKS
@ Registration Foyer

Take a quick coffee break.
18:00
18:30
WINE & CHEESE RECEPTION
@ Registration Foyer
18:00
Your Decision
DINNER ON OWN
@ Salzburg Old City

Enjoy Dinner in the Old Town of Salzburg.
18:30
20:00

PRIVATE: SHORT COURSE INSTRUCTOR DINNER & DISCUSSION
@ TBA

Monday

8:00
9:00
WELCOME COFFEE
@ Registration Foyer

Enjoy coffee, a pastry and a chat with colleagues before the day starts.
9:00
13:00

SHORT COURSES
Coffee Breaks every 0:50 for 10 minutes. Lunch from 13:00-14:00 in Registration Foyer.

Continued from Sunday
Data Science 101
Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Stephen Master, MD, PhD
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 5
Continued from Sunday
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 4
Continued from Sunday
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
Erik J. Soderblom, PhD & Chris Shuford, PhD
Level: 2 (Intermediate)
Location: Paracelsus
Continued from Sunday
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Papageno
Continued from Sunday
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: 1-2 (Beginner to Intermediate)
Location: Doppler
Continued from Sunday
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: 2-3 (Intermediate - Advanced)
Location: Trakl
Every 0:50
for 10 min
COFFEE BREAKS
@ Registration Foyer
13:00
14:00
LUNCH
@ Registration Foyer

Sandwiches provided in Registration Foyer.
14:00
18:00

SHORT COURSES
Coffee Breaks every 0:50 for 10 minutes.

COURSE START
Lab Medicine 101
Basics of Laboratory Medicine
Prof. Dr. med. Michael Vogeser
Level: 1 (Beginner)
Location: Mozart 2
COURSE START
Proteomic Microbiology 201
Bottom-Up and Top-Down Proteomic Approaches for Bacterial Identification and Characterization, a Focus on MALDI-TOF and Advanced Technologies
Jean-Armengaud, PhD, Stefan Zimmermann, MD
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 3
Continued from Monday AM
Data Science 101
Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Stephen Master, MD, PhD
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 5
Continued from Monday AM
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 4
Continued from Monday AM
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
Erik J. Soderblom, PhD & Chris Shuford, PhD
Level: 2 (Intermediate)
Location: Paracelsus
Continued from Monday AM
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Papageno
Continued from Monday AM
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: 1-2 (Beginner to Intermediate)
Location: Doppler
Continued from Monday AM
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: 2-3 (Intermediate - Advanced)
Location: Trakl
18:00
18:30
WINE & CHEESE RECEPTION
@ Registration Foyer
18:00
Your Decision
DINNER ON OWN
@ Salzburg Old City

Take Dinner in the Old Town of Salzburg.

Tuesday

8:00
16:00
PLACE POSTERS
@ Exhibit Hall on 1st Floor

ALL posters should be placed by 16:00.
8:00
9:00
WELCOME COFFEE
@ Registration Foyer

Enjoy coffee, a pastry and a chat with colleagues before the day starts.
9:00
12:30

SHORT COURSES

Continued from Monday PM
Lab Medicine 101
Basics of Laboratory Medicine
Prof. Dr. med. Michael Vogeser
Level: 1 (Beginner)
Location: Mozart 2
Continued from Monday PM
Proteomic Microbiology 201
Bottom-Up and Top-Down Proteomic Approaches for Bacterial Identification and Characterization, a Focus on MALDI-TOF and Advanced Technologies
Jean-Armengaud, PhD, Stefan Zimmermann, MD
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 3
Continued from Monday PM
Data Science 101
Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Stephen Master, MD, PhD
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 5
Continued from Monday PM
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: 1-2 (Beginner - Intermediate)
Location: Mozart 4
Continued from Monday PM
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
Erik J. Soderblom, PhD & Chris Shuford, PhD
Level: 2 (Intermediate)
Location: Paracelsus
Continued from Monday PM
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: 3 (Advanced)
Location: Papageno
Continued from Monday PM
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: 1-2 (Beginner to Intermediate)
Location: Doppler
Continued from Monday PM
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: 2-3 (Intermediate - Advanced)
Location: Trakl
Every 0:50
for 10 min
COFFEE BREAKS
@ Registration Foyer
12:30
14:00
OPENING LUNCH IN RECOGNITION OF TRAVEL GRANTEES
@ Europa Hall

13:30
13:45
WELCOME, INTRODUCTION & ORIENTATION
@ Europa Hall

Welcome to the 4th Annual European MSACL Congress!
13:45
14:00
CMS JOURNAL UPDATE
@ Europa Hall

An overview on the current state of the journal, how to subscribe, the path to an Impact Factor, citations, scope, desired article format, webinars.

Plenary Lecture Series
@ Europa Hall
Chair: TBA
14:00
14:45
Quantification of Proteins in Complex Biological Samples by LC-MS
Rainer Bischoff
University of Groningen
The quantification of proteins in complex biological samples is central to many areas of research, as well as to industrial and clinical applications. Especially the clinical chemistry laboratory uses quantitative protein assays on a daily basis to assist in medical decision making, for example, in defining the best therapy for a given disease or in disease diagnosis. While quantitative protein assays have relied and rely to a major part on ligand binding assays (LBAs) and in particular on immunoassays, we experience the advent of liquid chromatography – mass spectrometry (LC-MS) assays as an alternative or complement to LBAs. In this lecture I will delineate the advantages and shortcomings of LBAs and LC-MS assays with the goal of showing that there is no single approach that can answer all questions. I will notably address the point that proteins do not exist as single molecular entities in vivo and that we must therefore refer to proteins as families.
14:45
15:00
COFFEE BREAK
@ Registration Foyer
GENERAL SCIENTIFIC Session 1
Track 1
Mozart 1-3
Metabolomics
Chair: Elizabeth Want
Track 2
Mozart 4-5
Quality and Standardisation
Chair: Jody van den Ouweland
Track 3
Papageno Hall
TBA
Chair: Eva Hunyadi-Gulyas
Track 4
Paracelsus Hall
Practical Training: Basic
Chair: Laura Owen
Track 5
Trakl Hall
Practical Training: Intermediate
Chair: Roland Geyer
Track 6
Doppler Hall
Opens Wednesday
Chair: TBA
15:00
15:20
Altered Brain Metabolism in Alzheimer Disease: Linking Peripheral and Central Metabolic Changes
Hector Gallart-Ayala
Metabolomics Platform, University of Lausanne
Application of LC-MS/MS in Routine Clinical Diagnostics: Has it Lived Up the Expectations?
Eef Lentjes
University Medical Center Utrecht
Quantifying Proteins in Dried Blood Spots
Andy Hoofnagle
University of Washington
Basics of MS/MS
Laura Owen
University Hospital of South Manchester
Quality Assurance for Clinical LC-MSMS in Production - Why, What and How
Roland Geyer
University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Switzerland
Track Opens Wednesday
15:20
15:40
Mass Spectrometry-based Metabolic Profiling of Gut Microbiota Modulated Metabolites to Investigate Potential Health Effects on the Host
Zdenek Spacil
Masaryk University
Metrology for Clinical: From Small to Large
Christopher Hopley
LGC
How Low Can You Go? Using Flow to Measure Zero
Christopher Shuford
Laboratory Corporation of America
... continued from above... continued from aboveTrack Opens Wednesday
15:40
16:00
Proton-Transfer-Reaction Time-Of-Flight Mass Spectrometry (PTR-TOF-MS) Analysis of Volatile Organic Compounds in Human Gastric Cancer Tissue
Mina Adam
Imperial College London
Quality by Design in Bionanlysis: Elvitegravir Determination
Sara Baldelli
Clinical Pharmacology, L.Sacco University Hospital
Detection and Quantification of Carbohydrate Deficient Transferrin by MALDI-Compatible Protein Chips Prepared by Ambient Ion Soft Landing
Petr Pompach
Institute of Microbiology
... continued from above... continued from aboveTrack Opens Wednesday
16:00
16:15
COFFEE BREAK
@ Europa Foyer
16:15
16:45
POSTER LIGHTNING TALKS
@ Europa

Up to 15 posters will be selected to present their posters from the plenary podium within 90 seconds and 1-3 slides.
16:45
17:30
EXHIBITOR LIGHTNING TALKS
@ Europa

Up to 20 exhibitors will be selected to present their pitch from the plenary podium within 90 seconds and 1-3 slides.
17:30
EXHIBITS OPEN
@ Exhibit Hall on 1st Floor
17:30
20:00
EXHIBITOR RECEPTION
@ Exhibit Hall on 1st Floor

with a buffet dinner served in the Exhibit Hall.
18:00
19:00
MEET-THE-EXPERTS: Booth Tours
@ Exhibit Hall on 1st Floor

Join an expert as they tour the booths and learn more about technologies available, what experts are looking for during the exchange, and how they interact with the booth vendors. Increase your network and your knowledge of products and services available.
19:00
20:00
TROUBLESHOOTING POSTER ROUNDS Part I
@ Exhibit Hall on 1st Floor
20:00
21:00

DISCUSSION GROUP: The Future of MS in the Clinical Lab
... with a touch of wine and cheese, and other beverages.
@ Papageno

21:00
Your Decision
ENJOY THE CITY
@ Salzburg Old City

Explore the Old Town of Salzburg.

Wednesday

8:00
8:30
WELCOME COFFEE
@ Registration Foyer

Enjoy coffee and a pastry before joining a corporate workshop.
8:15
8:45

CORPORATE WORKSHOPS

Thermo Scientific
Mozart 4-5

Introducing the Thermo Scientific™ Cascadion™ SM Clinical Analyzer: Accuracy. Ease-of-Use. Designed for the Clinical Lab
Sarah Robinson, PhD, Market Development Specialist, Thermo Fisher Scientific

The Cascadion SM Clinical Analyzer is the first all-in-one LC-MS/MS solution designed to meet the needs of clinical laboratories. Our fresh vision for fully automated liquid chromatography-tandem mass spectrometry (LC-MS/MS) testing was cultivated by listening to our customers. You asked for a complete solution that was accurate, easy-to-use, and designed for the clinical laboratory. The Cascadion Clinical Analyzer is designed as a turnkey solution to enable clinical labs to easily adopt the power and capabilities of LC-MS/MS as the gold standard in diagnostic testing. The Cascadion system combines assays, software, accessories, consumables and support/service in a standalone system designed to meet the regulatory requirements for routine and specialized clinical testing. This presentation will showcase the Cascadion solution.
This product is in development and not yet available for sale. This product is not CE marked or FDA 510(k) cleared.

SCIEX
Papageno

Pre-Register

SWATH®-MS for Quantitation and Identification in Clinical Proteomics, Lipidomics, and Metabolomics

SWATH®-MS, a data-independent MSMS analysis (DIA) was originally developed for proteomics, to quantify thousands of tryptic peptides during one LC run without selection of a given precursor ion. Using a database containing the experimentally measured MSMS spectra, quantitative analysis of proteins has been achievable for several years. Recent software developments have permitted protein quantification to be performed in tandem with protein identification.
In lipidomics, also a complex class of compounds, the MSMS behavior of certain lipid groups is often characterized by isobaric precursors and daughter ions, but different structures. This precludes using normal SWATH®-LC applications, but by introducing ion mobility analysis with a DIA approach, isobaric lipids can be separated and quantified. By coupling direct infusion on a TripleTOF® 6600 system with high field ion mobility (using a SelexION® DMS cell), DIA is performed in 1 Da windows (MSMSALL).
The pros and cons will be shown for all the three fields of clinical proteomics, lipidomics, and metabolomics where the high numbers of samples require a robust method resulting in the use of micro-LC.

8:30
9:00
COFFEE BREAK
@ Europa Foyer

Enjoy coffee before attending a Plenary Lecture.
Plenary Lecture Series
@ Europa Hall
Chair: Russell Grant
9:00
9:45
Smaller, Better, Faster Metabolic Phenotyping Using LC-MS and LC-IMS-MS
Ian Wilson
Imperial College London
For the discovery of biomarkers via MS and LC/MS-based metabolic phenotyping (metabotyping) studies employing untargeted metabonomic/metabolomic methods there is always a tension between maximising throughput and obtaining the most comprehensive metabolic profiles possible. In particular, rapid profiling methods can suffer from ion suppression, leading to analytes being missed, whilst “deep” profiling is time consuming. The introduction of ultra (high) performance LC provided a significant improvement in efficiency and enabled many more metabolites to be detected per unit time compared to conventional HPLC. This presentation will explore further advances resulting from the use of miniaturisation and the combination of U(H)PLC-MS with ion mobility spectrometry (IMS) to enhance the efficiency of metabotyping for both rapid and comprehensive profiling approaches.
9:45
10:45
POSTERS ATTENDED
@ Exhibit Hall on 1st Floor

Selected posters to be attended for 1 hour. Refer to program for posters attended during this period.
GENERAL SCIENTIFIC Session 2
Track 1
Mozart 1-3
Analytical Methods in Metabolomics
Chair: Antonis Myridakis
Track 2
Mozart 4-5
Androgens
Chair: TBA
Track 3
Papageno Hall
Recent trends in whole-cell MALDI-TOF
Chair: Simon Cameron
Track 4
Paracelsus Hall
Practical Training: Basic
Chair: Michael Vogeser
Track 5
Trakl Hall
Practical Training: Intermediate
Chair: Russell Grant
Track 6
Doppler Hall
Intraoperative Applications
Chair: Kristina Schwamborn
10:45
11:05
Simultaneous Quantification of Oxysterols and Bile Acids in Human Plasma by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry
Madlen Sander
Leipzig University
Investigation of Androgen Excess in Women
Brian Keevil
University hospital of South Manchester
Mass Spectrometry in the Micro Lab Workflow Beyond Identification
Stefan Zimmermann
Department of Infectious Diseases
Basics of LC
Michael Vogeser
Hospital of the University of Munich, Germany
How to Achieve Lower Quantification Limits
Russell Grant
Laboratory Corporation of America
Multimodal Molecular Profiling by Mass Spectrometry (Imaging): On its Way to Clinics?
Tiffany Porta
Maastricht University
11:05
11:25
Pre-Analytic Evaluation of Volumetric Absorptive Microsampling and Integration in a Mass Spectrometry Based Metabolomics Workflow
Giuseppe Paglia
EURAC Research
A Broad LC-MS/MS Serum Profiling Revealed a Specific Steroid Pattern in Hirsute Women with Polycystic Ovary Syndrome
Flaminia Fanelli
University of Bologna - S.Orsola-Malpighi Hospital
Beyond “Bacterial Identification Revolution” – Detection of Resistance Markers by MALDI-TOF MS
Markus Kostrzewa
Bruker Daltonik GmbH
... continued from above... continued from aboveNondestructive Tissue Analysis for ex vivo and in Vivo Cancer Diagnosis Using a Biocompatible Mass Spectrometry System
Marta Sans
The University of Texas
11:25
11:45
Investigating the Role of Collision Cross Section (CCS) in Metabolomics: How is CCS Used for Metabolite Identification?
Charles Nichols
Vanderbilt University
Biological Variation of Testosterone, Dihydrotestosterone and their Ratio Determined in 30 Healthy Individuals with LC-MS/MS
Anna van der Veen
University Medical Center Groningen
Detection of Carbapenem-Resistance in Bacteroides fragilis by MALDI-TOF MS – a Quantum Leap
Miriam Cordovana
University Hospital Sant'Orsola-Malpighi, Bologna
... continued from above... continued from aboveIdentification of Cancerous and Precancerous Cervical Abnormalities in Real-Time with the Use of Rapid Evaporative Ionization Mass Spectrometry (REIMS)
Menelaos Tzafetas
Imperial College London
11:45
12:00
COFFEE BREAK
@ Europa Hall Foyer

Take a quick coffee before attending the plenary in Europa Hall.
Plenary Lecture Series
@ Europa Hall
Chair: TBA
12:00
12:45
Laser-based Mass Spectrometry in Clinics: Changing the Paradigm of Molecular Diagnosis
Isabelle Fournier
Laboratoire PRISM U1192, INSERM

Distinguished Contribution Awardee

By bridging surface analysis with access to biomolecules of various molecular weight and polarity including higher molecular weight polar compounds such as proteins, Matrix Assisted Laser Desorption Ionization (MALDI) reseals a unique ability for clinical applications. This was clearly demonstrated by the introduction and development of MALDI MS Imaging (MALDI MSI) which after 15 years has now acquired its letters of nobility. Indeed deploying dedicated strategy MALDI MSI can be used to image the distribution of both endogenous and endogenous molecules within biological tissues to access a vast variety of applications in the field of basic sciences as well as clinics. In clinics, MALDI MSI starts to be employed as a molecular histology tool allowing to perform retrospective and prospective studies on patient’s cohorts for biomarkers hunting, as a new diagnosis and prognosis tool as well as for patient’s classification or stratification in the objective to get personalized medicine. However, such laser based technology can also be translated to the in-vivo context for developing MS as a tool for guided surgery in the surgery room.
12:45
14:00
LUNCH
@ Exhibit Hall on 1st Floor

Lunch to be provided in the Exhibit Hall. Get ready to join a Corporate Workshop after lunch.
14:00
14:30

CORPORATE WORKSHOPS

Shimadzu
Mozart 4-5

Pre-Register

Disruptive Technologies and Creative Solutions in Clinical Mass Spectrometry

1. Application of the fully-automated sample preparation module, CLAM-2000, in clinical diagnostics
Dr. Thomas Stimpfl, Medical University of Vienna - Department of Laboratory Medicine
In clinical diagnostics random access to wide range of target analytes is needed. We will present experience with a novel, fully-automated sample preparation module - directly connected to a LC-MS-system - in the routine setting of a university hospital laboratory.

2. Rethinking library identification in quantitative clinical toxicology - transitioning towards MRM spectrum mode
Neil Loftus - SHIMADZU Corporation (UK)
Translating information-rich mass spec data into meaningful test results is a hurdle in many labs. To help transition toward high-confidence result reporting we have been rethinking the use of MRM in compound identification. We will show how MRM can help reduce false-positive and false-negative reporting without affecting quantitative data quality.

For Research Use Only. Not diagnostic use. Not available in USA or Canada. This presentation may contain references to products not available in your country. All rights reserved. Information subject to change without notice.

SCIEX
Papageno

Pre-Register

Introducing the New SCIEX Topaz™ System, a CE-marked Integrated LC-MS-based IVD System

SCIEX is proud to launch the new TOPAZ System for Clinical Diagnostics at MSACL EU 2017. In this seminar, we will present the system and its capabilities, in particular the new ClearCore MD software designed specifically for clinical diagnostics. We will show how the system, based on strong and established mass spectrometry technology, brings enhanced ease-of-use to the diagnostic laboratory, exploiting the power of mass spectrometry to address the diagnostic challenges of today and tomorrow. Seminar delegates will have the opportunity to see and discuss real-world examples of the multitude of diagnostic applications the TOPAZ system is capable of, and meet European leaders in the diagnostics arena as well as SCIEX Diagnostics global experts in the field.

Waters
Paracelsus

Pre-Register

Protein Quantification in the clinical laboratory. Challenges and opportunities for improved assays with LC/MS.

Although LC/MS has been successfully used and widely adopted for many small molecule clinical assays, the technique has yet to make a big impact for peptide and protein quantitation applications in the clinical laboratory. With increasingly sensitive instruments and robust micro fluidic inlet devices combined with efficient and standardised sample pre-treatment protocols LC/MS may now be ready for routine protein applications. Challenges and opportunities for LC/MS in protein and peptide biomarker assays and therapeutic drug monitoring of monoclonal antibody (mAb) drugs will be presented.

Neoteryx
Trakl

Pre-Register

This workshop examines how two experts used Volumetric Absorptive Microsampling (VAMS™) technology to generate results comparable to those from wet blood, using a variety of analytes and eliminating the hematocrit bias associated with traditional Dried Blood Spot (DBS) cards and filter paper.

Volumetric Absorptive Microsampling (VAMS) and LC-MS/MS Analysis for Simultaneous Monitoring of 16 Antiepileptic Drugs: Workflow Development and Validation
Dr. Ugo de Grazia, Nazionale Neurologico Carlo Besta, Italy

Steroid Collection Using the Mitra Microsampling Device
Prof. Brian Keevil, University Hospital of South Manchester, United Kingdom

14:30
14:45
COFFEE BREAK
@ Registration Foyer and Exhibit Hall

GENERAL SCIENTIFIC Session 3
Track 1
Mozart 1-3
Metabolite Profiling
Chair: Hector Gallart Ayala
Track 2
Mozart 4-5
Endocrine
Chair: TBA
Track 3
Papageno Hall
Clinical Proteomics in Cancer and Immune-related Diseases
Chair: TBA
Track 4
Paracelsus Hall
Practical Training: Basic
Chair: Judy Stone
Track 5
Trakl Hall
Practical Training: Intermediate
Chair: Brian Rappold
Track 6
Doppler Hall
Applications in Routine Pathology
Chair: Isabelle Fournier
14:45
15:05
Optimisation and Validation of a High-Throughput Semi-Targeted Method by GC-MS with Metabolite Libraries for Large Scale Molecular Epidemiological Research
Antonis Myridakis
Imperial College London
Single Assay Measurement of the Aldosterone-Renin-Ratio by Online SPE-UHPLC-MS/MS
Nicola Gray
Shimadzu UK
Deciphering Molecular Consequences of the Prohormone Convertase 1/3 Inhibition in Macrophages for Application in Immunotherapy
Michel Salzet
Laboratoire PRISM U1192, INSERM
Basics of Sample Preparation - Remaining Unfazed while Your Analytes Move from Phase to Phase
Judith Stone
Univ. of Calif. San Diego Health System
Pragmatic HILIC for the Clinic
Brian Rappold
Essential Testing
Imaging Mass Spectrometry - Possible Applications in Pathology
Kristina Schwamborn
Institute of Pathology, TU Munich
15:05
15:25
Quantification of Urinary Organic Acids Using Liquid Chromatography- Tandem Mass Spectrometry
Gökçe Göksu Gürsu
JASEM Laboratory systems and solutions
Interference from Alpha- and Beta-Dihydrocortisone in Salivary Cortisol Assays: Ramifications for Patient Care
James Hawley
University Hospital South Manchester
Comprehensive Proteomic Analysis of the Psoriatic Non-Lesional and Healthy Skin with Label-Free Semi-Quantitative Approach
Eva Hunyadi-Gulyas
Laboratory of Proteomics Research, BRC, HAS
... continued from above... continued from aboveDifferentiating Benign and Malignant Endometrial Tissue Subtypes Using Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI-MSI)
Tanja Farrokh-Eslamlou
Imperial College London
15:25
15:45
Accurate and Confident Metabolic Phenotyping - Combining a Standardized and Quantitative Targeted Assay with Orbitrap™ Technology
Therese Koal
BIOCRATES Life Sciences AG
Derivatization as a Tool for Reaching Low pg/mL Detection Limits in Salivary Steroidomics
Cato Brede
Stavanger University Hospital
Quantification of PI3K p110a, PTEN and AKT I+II in Colorectal Cancer Cell Lysate and Tissue Samples Using Immuno-MALDI (iMALDI)
Björn Fröhlich
UVic Genome BC Proteomics Centre
... continued from above... continued from above3D Mass Spectrometry Imaging of Human FFPE Bladder Cancer Resections
Naomi Vos
M4I division of IMS, Maastricht University
15:45
16:00
COFFEE BREAK
@ Registration Foyer and Exhibit Hall

16:00
16:30

CORPORATE WORKSHOPS

Thermo Scientific
Mozart 4-5

Quantitation of endocrine markers using High Resolution Mass Spectrometry
Laura Owen – University Hospital of South Manchester
Traditionally the quantitation of endocrine biomarkers has been performed using triple quadrupole analysers whereas orbitrap technology lends itself primarily to drug monitoring and screening applications. However, in recent years, orbitrap high resolution mass spectrometry systems have proved to be a great screening and quantitation tools.
Our research goal was to quantify renin activity, aldosterone and metanephrines using orbitrap HRMS, in order to bring an added level of capability and flexibility to our lab. For plasma renin analysis, data was acquired in both PRM and full scan modes while, for aldosterone analysis, extract was analysed by PRM only with negative ion mode. Renin activity was measured at concentration level typically found in Conn’s syndrome with sufficient sensitivity. PRM showed slightly superior sensitivity than full scan mode. Metanephrine and normetanephrine both gave adequate sensitivity and calibration lines however 3-methoxytyramine was not sufficiently sensitive. Aldosterone demonstrated good sensitivity in sub 100pmol/L concentrations.
The QE focus demonstrated its ability to quantify difficult to measure endocrine markers offering quantitation capabilities along with full scan thus bringing screening, protein discovery and quantitative capabilities to the clinical laboratory.

Tecan
Papageno

Pre-Register

Automation in Clinical Labs

1. Automation and Integration of LC/MS/MS Assays into the Workflow of a Clinical Laboratory
Emma Walker (Imperial College Healthcare NHS Trust)
Automation of sample preparation and interfacing of the LC-MS/MS system to the laboratory information system has the potential to reduce staff time and increase sample throughput. In her talk, Emma will present the approaches she has taken to streamline sample extraction utilizing Tecan Freedom Evo platforms within her laboratory. For example the endocrinology service is using the automated SPE 96-well plates format to screen patients for testosterone, androstenedione and 17-hydroxyprogesterone. The steroid panel assay runs two or three times a week, and it takes approximately 90 minutes to prepare for analysis - short overview about workflow, turnaround times, efficiency and standardization.

2. Drug Screening in Whole Blood Using Fully Automated SPE and UHPLC-TOF-MS
Brian Schou Rasmussen (University of Copenhagen, Forensic Chemistry)
Brian will present experiences from the automation of sample preparation in a forensic toxicology LC-MS analysis on a robotic platform. Workflow strategies, sample throughput, cost efficiencies and special considerations concerning forensic samples with be discussed.

Agilent Technologies
Paracelsus

Pre-Register

High density lipoprotein (HDL) plays a number of roles in vascular biology, including lipid and cholesterol transport, anti-oxidative and anti-thrombotic activity, endothelial regulation, and immune response regulation. Cholesterol efflux capacity (CEC), the transport of intracellular cholesterol from macrophages to an accepting HDL particle. The CEC assay is a challenging cell based assay that is labor intensive and low throughput. To enable research investigating CEC and its role in atherosclerotic plaque development we have developed a multiplex proteomic approach to estimating cholesterol efflux from a measurement of six lipoproteins.

16:30
16:45
COFFEE BREAK
@ Registration Foyer and Exhibit Hall

GENERAL SCIENTIFIC Session 4
Track 1
Mozart 1-3
Metabolite Profiling Methods
Chair: Roland Geyer
Track 2
Mozart 4-5
Vitamin D
Chair: Flaminia Fanelli
Track 3
Papageno Hall
Revolutionizing Pathogen Detection by LC-MS/MS Proteotyping
Chair: Stefan Zimmerman
Track 4
Paracelsus Hall
Practical Training: Basic
Chair: Michael Wright
Track 5
Trakl Hall
Practical Training: Intermediate
Chair: Chris Shuford
Track 6
Doppler Hall
New Technologies
Chair: Tiffany Porta
16:45
17:05
Application of LC-MS/MS Metabolomics - Quantitative High-Throughput Method for Direct Measurements of Enterolactone Glucuronide, Sulfate and Free Enterolactone
Natalja Pustovalova Nørskov
Aarhus University
A Fast and Simple Method for Simultaneous Measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in Serum Samples by LC-MS/MS
Neus Fabregat-Cabello
University of Liège, CHU de Liège
Identification of Microorganisms Grown in Blood Culture Flasks Using LC-HRMS/MS
Erwin Berendsen
TNO
System Suitability Tests Part 1: Introduction to System Suitability Tests
Michael Wright
LGC, Drug Development Services
Getting Started with Protein Quantitation
Christopher Shuford
Laboratory Corporation of America
The Implementation of Needle Electrospray Ionisation Mass Spectrometry for the Analysis of Aspiration Fluid Cytology in Locally Advanced Oesophageal Cancer
Pranav Patel
Imperial College London. London, UK.
17:05
17:25
Development and Validation of an LC-MS/MS Method for Measurement of Methylmalonic Acid and Homocysteine in Human Plasma
Forough Bahadory
SEALS, Department of Clinical Chemistry
Time-Course Analysis of 3-epi-25-Hydroxyvitamin D3 Shows Markedly Elevated Levels in Early Life, Particularly from Vitamin D Supplementation in Preterm Infants
Jody van den Ouweland
Canisius-Wilhelmina Hospital
Proteotyping: Tandem Mass Spectrometry Shotgun Proteomic Characterization and Typing of Pathogenic Microorganisms
Roger Karlsson
Sahlgrenska Academy, University of Gothenburg
System Suitability Tests Part 2: Troubleshooting Common Issues
Michael Wright
LGC, Drug Development Services
... continued from aboveCan Real-Time Molecular Analyses Change the Paradigm of Dog Sarcoma Diagnosis and Classification?
Isabelle Fournier
Laboratoire PRISM U1192, INSERM
17:25
17:45
Lipidomic Evaluation of Cultured Cell Lines
Finnur Freyr Eiriksson
University of Iceland
SPME as Sample Preparation Technique Applied in Prohibited Substances Analysis from Various Matrices – Promising Tool for Doping Control Laboratories?
Krzysztof Gorynski
Nicolaus Copernicus University Collegium Medicum
Metaproteomics for the Clinics: Quick Survey of Microbiota by MS/MS
Jean Armengaud
CEA
System Suitability Tests 3: Case Studies
Michael Wright
LGC, Drug Development Services
... continued from aboveChemical Scanning of Cancer Tissues by the Combination of Mass Spectrometry with Molecular Resonance Probe Desorption
Vladimir Frankevich
Research Center for Obstetrics and Gynecology
17:45
19:30
RECEPTION
@ Exhibit Hall on 1st Floor

Enjoy mingling with colleagues and Exhibitors. Take time to browse the posters. Buffet dinner, appetizers and drinks to be provided.
18:30
19:30
POSTERS ATTENDED
@ Exhibit Hall on 1st Floor

Selected posters to be attended for 1 hour. Refer to program for posters attended during this period.
18:30
19:30
MEET-THE-EXPERTS: Poster Tours
@ Exhibit Hall on 1st Floor

Join an expert as they tour the posters that they find interesting and participate in a dynamic discussion.
19:30
20:30

DISCUSSION GROUPS
... with a touch of wine and cheese, and other beverages.
@ Mozart 1-3, 4-5, Papageno, Paracelsus, Trakl

Design of Experiments - Get it right from the beginning
TBD
Lead(s): Margrét Thorsteinsdóttir

We will demonstrate how method development can become much more efficient by utilizing design of experiments (DoE) approach. DoE offers many advantages including performing experiments in accordance to predefined plan, modelling by empirical functions and graphical visualization. Example from optimization of a LC-MS/MS clinical diagnostic method will show that DoE is very cost-effective, allowing the effect of variables to be assessed with only a fraction of the experiments that would be required by changing one-separate-factor-at-time (COST) approach and spots critical setting early on.

Mass Spectrometry Data Standards Discussion
TBD
Lead(s): Patrick Mathias

Defining data standards for mass spectrometry that are specific to clinical laboratory operations will speed adoption of mass spectrometry into the clinical laboratory by facilitating ease of data transfer (from instrument to laboratory information system and vice versa). While mass spectrometry offers superior analytic performance, the ease of data transfer between instruments and clinical laboratory information systems is inferior to conventional high volume analyzers. This is due in large part to the lack of standardized data formats (file formats, specifications for file exports, etc.) and limited availability of interfaces to common laboratory information systems or middleware. These barriers increase the effort required for adoption and scaling of clinical mass spectrometry and require individual clinical laboratories to "reinvent the wheel" in developing interface solutions (if the resources to do so are even available). Our Mass Spectrometry Data Standards workgroup aims to lower these barriers to integrating mass spectrometry into the clinical laboratory by defining data standards that are specific to clinical laboratory applications. We are gathering stakeholder input from a broad spectrum including end users, mass spectrometry vendors, liquid handlers, and laboratory information system/middleware vendors to characterize current practices. In this discussion we will summarize the current issues the workgroup is aware of, solicit input from stakeholders, and identify key issues for our workgroup to address in developing these standards.

Scientific Committee Review
TBD
Lead(s): David Herold
20:30
Your Decision
ENJOY THE CITY
@ Salzburg Old City

Explore the Old Town of Salzburg.

Thursday

8:00
9:00
WELCOME COFFEE
@ Registration Foyer

Enjoy coffee and a pastry before joining a corporate workshop.
8:15
8:45

CORPORATE WORKSHOPS

No workshops currently booked for this time period.

GENERAL SCIENTIFIC Session 5
Track 1
Mozart 1-3
Clinical Metabolomics
Chair: Oleg Mayboroda
Track 2
Mozart 4-5
TDM/DOA
Chair: Michael Vogeser
Track 3
Papageno Hall
Proteomic Applications
Chair: Andy Hoofnagle
Track 4
Paracelsus Hall
Practical Training: Basic
Chair: Erik Soderblom
Track 5
Trakl Hall
Practical Training: Intermediate
Chair: Carina Horro Pita
Track 6
Doppler Hall
Track Closed
Chair: TBA
9:00
9:20
Diagnosis of Multiple Myeloma by Mass Spectrometry of Peripheral Blood Plasma and Artificial Intelligence
Meritxell Deulofeu
Masaryk University
GC-APCI-QTOFMS Coupled to Nitrogen Chemiluminescence Detector: A Tool for Identification and Quantification of New Psychoactive Substances and their Metabolites
Samuel Mesihää
University of Helsinki
Quantitative Proteome Analysis Applied to Human Arterial Tissue: Lessons in Relation to Cardiovascular Diseases
Lars Melholt Rasmussen
Odense University Hospital
Basics of LC-MS Troubleshooting
Erik Soderblom
Duke University
Where Did My Analyte Go? – Coping with Poor Solubility and Non-Specific Binding
Catarina Horro Pita
LGC - Drug Development Services
Track Closed
9:20
9:40
Vaginal Swabs Analysis Using Desorption Electrospray Ionization (DESI) MS Permits Rapid Identification of Metabolic Signatures Associated with Preterm Birth
Holly Lewis
Imperial College London
Novel LC-MS Approaches to Determination of Chemical Warfare Agents and Related Compounds in Biomedical Samples
Igor Rodin
Moscow State University
Quantitative Analysis of Phosphorylation Sites in the Platelet Proteome After ADP Stimulation
Christin Lorenz
Leibniz-Institut für Analytische Wissenschaften
... continued from above... continued from aboveTrack Closed
9:40
10:00
Evidence of an Impairment of Cholesterol Metabolism Associated to Mitochondrial Dysfunction. A Metabolomic Approach Based on Mass Spectrometry
Valerio Leoni
lab of Clinical Chemistry, Hospital of Varese
Fake News, Alternative Facts or Just Normal Pharmacokinetics? High Urine Naloxone Concentrations in Patients Prescribed Sublingual Buprenorphine-Naloxone (BNX)
Judith Stone
Univ. of Calif, San Diego Health System
Clinical Applications of Universal S-Trap Sample Processing
John Wilson
Cold Spring Harbor Laboratory
... continued from above... continued from aboveTrack Closed
9:00
10:00
EXHIBITOR FEEDBACK SESSION
@ 1st Floor - Lower Cafe
10:00
11:00
POSTERS ATTENDED
@ Exhibit Hall on 1st Floor
10:00
11:00
COFFEE BREAK
@ Exhibit Hall
GENERAL SCIENTIFIC Session 6
Track 1
Mozart 1-3
Closing Metabolomics Keynote
Chair: TBA
Track 2
Mozart 4-5
Other Topics
Chair: Christiane Auray-Blais
Track 3
Papageno Hall
Innovation for the Clinical Microbiology Lab
Chair: Jean Armengaud
Track 4
Paracelsus Hall
Track Closed
Chair: TBA
Track 5
Trakl Hall
Track Closed
Chair: TBA
Track 6
Doppler Hall
Track Closed
Chair: TBA
11:00
11:20
Discovery, Validation and Implementation of Biomarkers for Clinical Metabolomics
David Wishart
University of Alberta
Towards Combined Determination of Paracetamol and APAP-Cys in VAMS via LC-MS/MS
Lisa Delahaye
Ghent University
Laser Assisted Rapid Evaporative Ionisation Mass Spectrometry (LA-REIMS): An Automated High-Throughput Platform for Clinical Microbiology and Beyond
Simon Cameron
Imperial College London
Track ClosedTrack ClosedTrack Closed
11:20
11:40
... continued from aboveUrine Oxalate & Citrate; How Do We Get the Correct Answer?
David Marshall
University Hospital South Manchester
Defining Systemic Bacterial Infection Through Construction of a Murine Organ Atlas
John Lapek
University of California, San Diego
Track ClosedTrack ClosedTrack Closed
11:40
12:00
... continued from aboveIsoform-Specific Quantitation of Human Growth Hormone via a Bead-based MSIA Assay
Julia Dittrich
University Hospital Leipzig
Paper Substratum for MALDI-TOF Disposable Targets
Nadine Perrot
R&D Microbiology Department, bioMérieux S.A.
Track ClosedTrack ClosedTrack Closed
12:15
14:00
MEET-THE-EXPERTS: OFFICE HOURS FORUM
@ Cafe: Lower Level

Have a question from the congress that you have been itching to ask? Or a problem from work that you want to get feedback on? Or feedback on the congress? Sign up at the registration desk for 10-min blocks to share time and ideas with domain experts in a relaxed setting.
12:00
14:00
LUNCH RECEPTION
@ Exhibit Hall on 1st Floor

with foosball tournament.
13:00
14:00
TROUBLESHOOTING POSTER ROUNDS Part II
@ Exhibit Hall on 1st Floor

Review the Troubleshooting Posters with an expert docent as they explore and investigate troubleshooting issues in clinical mass spectrometry.
14:00
EXHIBITS CLOSED
@ Exhibit Hall on 1st Floor
14:00
14:15
POSTER AWARD PRESENTATION
@ Mozart
Chair: TBA
Plenary Lecture Series
@ Mozart
Chair: TBA
14:15
15:00
Prospects of Apolipoprotein Profiling for Precision Cardiovascular Diagnostics
Christa Cobbaert
Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Centre, Leiden, the Netherlands
Lipid abnormalities account for more than 60% of the population attributable risk for myocardial infarction and are the most important single target for prevention, together with smoking cessation and blood pressure lowering. Although statin therapy is the cornerstone of dyslipidemia management, LDLc lowering with statins reduces major coronary events by only one quarter, with 75% of events still occurring. Dyslipidemic patients remain at high Residual vascular Risk despite treatment for high LDLc in accordance with current standards of care. Atherogenic dyslipidemia is a key factor associated with Residual vascular Risk. So far, atherogenic dyslipidemia is largely under-diagnosed and under-treated in clinical practice as only overall lipid classes are measured rather than detailed molecular entities with specific functionalities. Comprehensive protein biomarkers are needed to obtain a better understanding of incident cardiovascular disease, but also for improved patient selection for evaluating treatment efficacy and safety. Recent mass spectrometry advances enable deep apolipoprotein proteomics to uncover mechanisms of coronary disease risk.
15:00
16:30
HOSPITALITY
@ Registration Foyer

Wine & Cheese, Beer & Nuts.
15:00
16:30
POSTER AWARD VIEWING
@ Registration Foyer

View the winning posters while taking some time to relax with friends and colleagues.
16:30
CONGRESS CLOSED
@ Congress Center
16:30
23:00
After Congress Meet-Up
@ Augustiner Brauhaus

Unofficial and unsponsored post-con gathering at the AugustinerBrauhaus.