MSACL 

Quantification of Perchlorate and Related Anions in Human Matrices using IC-MS/MS
Wed 8:30 AM - Track 3: Inorganic Analytes
Benjamin Blount
CDC
Liza Valentín-Blasini, Samaret Otero-Santos, Ben Blount.

Division of Laboratory Sciences, Centers for Disease Control and Prevention
Because of health concerns surrounding widespread exposure to perchlorate, we developed a sensitive and selective method for quantifying perchlorate and toxicologically-related anions (iodide, nitrate, thiocyanate) using ion chromatography coupled with electrospray ionization tandem mass spectrometry. These analytes were quantified using stable isotope-labeled internal standards (Cl18O4–, SC15N–, 15NO3–, and 129I–) for perchlorate, thiocyanate, nitrate, and iodide, respectively.

Excellent analytical precision was found when urine quality control pools were repetitively analyzed for perchlorate, thiocyanate, nitrate and iodide. Selective chromatography and tandem mass spectrometry reduced the need for sample cleanup, resulting in a rugged and rapid method capable of routinely analyzing 75 samples/day. Analytical response was linear across the physiologically relevant concentration range for the analytes.

This selective, sensitive and rapid method will help assess the health relevance of human exposure to perchlorate, nitrate and thiocyanate. The analytical method has also been adapted to enable quantification of perchlorate and related anions in a variety of physiological fluids (amniotic fluid, cord blood, dried blood spots, and diaper press urine) relevant to sensitive life stages (developing fetus and neonates).
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