MSACL 

Human Biomonitoring of Perfluorochemicals in Communities with Contaminated Drinking Water in the East Metro of St. Paul, Minnesota
Wed 10:30 AM - Track 3: Environmental Biomonitoring
Carin Huset
Minnesota Dept of Health
*Carin Huset, #Adrienne Kari, *Martin Bevan, *Andrew Mittendorf, *Paul Swedenborg,*Louise Liao, #Allan Williams, #Jean Johnson

*Public Health Laboratory, Minnesota Department of Health
#Chronic Disease and Environmental Epidemiology, Minnesota Department of Health
In 2007, the Minnesota Legislature established a state biomonitoring program, directing the Minnesota Department of Health to complete a series of biomonitoring pilot projects. One of these projects was to measure perfluorochemical (PFC) levels in 100 voluntary participants in each of two communities likely to be exposed to PFCs, including PFBA. Prior to 2007, the State of Minnesota found PFC contamination of the ground water in the East Metro of Saint Paul (and specifically in Washington County). The Minnesota Pollution Control Agency and the Minnesota Department of Health had analyzed water samples throughout the East Metro area to determine the levels of PFOA, PFOS, PFBA, and other PFCs in private and municipal wells.

With this information, the Minnesota Department of Health selected two communities for the East Metro PFC Biomonitoring Pilot Project. Recruitment began in July 2008. Participants were randomly selected from eligible households. Eligibility was based on drinking water contamination and residence prior to Jan 1, 2005. To complete the study requirements, the participants answered a short phone survey and provided a serum specimen at a local clinic; 98 participants from each community were enrolled. Serum specimens were collected and analyzed for a suite of seven PFCs using HPLC/MS/MS. We combined aspects of the method that CDC had developed to measure 13 PFCs in human serum with the method that our Minnesota Department of Health laboratory had developed to measure 7 PFCs in water samples. (These are overlapping sets of PFC species.) Serum specimens were extracted using a previously published solid phase extraction method with modifications to ensure recovery of short chained analytes (PFBS & PFBA). Extracts were analyzed using a LC/MS/MS system. Quantification of extracts was performed using bovine calf serum for matrix-matched calibration curves and stable isotope labeled internal standards. Of the 7 analytes characterized in the 196 serum samples, 3 analytes (PFOA, PFOS, and PFHxS) were detected in all samples, 1 analyte (PFBA) was detected in 55 samples, 1 analyte (PFBS) was detected in 5 samples, and the final 2 analytes (PFPeA and PFHxA) were below the limit of detection (0.1 ppb) for all 196 samples . The presentation will include an analysis of results and comparisons to other populations and to East Metro drinking water levels.
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