20. Rapid Microorganism Identification by MALDI Mass Spectrometry
Protein Biomarker of Ovarian Cancer Tumor Burden
Tue 4:54 PM - PosterSplash Track 2
Fan Xiang (replacing Scott Kuzdzal)
Scott A. Kuzdzal, Shimadzu Scientific Instruments, Columbia, MD, USA
Emmanuel Raptakis, Shimadzu Biotech, Manchester, UK
Joachim Dyck, AnagnosTec GmbH, Potsdam, Germany
Matrix-assisted laser desorption/ionization Time-of-Flight (MALDI-TOF) mass spectrometry has become one of the most powerful and widely applied technologies for genetic and proteomic applications. MALDI-TOF MS offers a new and promising approach to the rapid identification of pathogens and offers powerful advantages over traditional biochemical and genetic approaches. Identification of microorganisms by MALDI-TOF MS can be applied to a wide array of bacteria, yeasts, and molds and does not require laborious cultivation steps. A review of microorganism ID by MALDI-TOF MS will be given, including basic principles of sample preparation, spectra acquisition and database identification.