33. Rapid High-Throughput LC/MS/MS Assays for 13 Commonly Prescribed Pain Management Drugs and 11 Drugs of Abuse (Expanded SAMHSA) from Urine with Cleanup Using Solid Phase Extraction (SPE)
Poster: Mon 2:00-3:00PM
Michael Campognone
Michael Campognone, Carl Sanchez, Philip J. Koerner, Sky Countryman

Phenomenex, Inc., Torrance, CA, USA

In clinical and forensic toxicology cost pressures and increasing sample analysis requirements (additional analytes and lower reporting levels combined with the need for increased sample throughput and reproducibility) have initiated a mass migration from the traditional GC/MS analysis to LC/MS/MS platforms. The driving forces include the need for speed, sensitivity, reduced labor/ resources and cost, improved profitability, and faster turnaround time for timely delivery of data and results to clients. The ability to easily screen substantially more compounds in the same rapid method with fewer necessary modifications provides additional value to the client and improves profit margins for competitive services offered. Screening smaller patient sample volumes for more compounds in the same analysis reduces cost and dramatically improves productivity and profitability for the contract research organization. Furthermore, implementing nearly identical LC/MS/MS methods for different drug screening protocols simplifies method development and routine analysis, while improving sample throughput, reducing error and the incidence of costly reanalysis.

Utilizing the ultra-high efficiency provided by core-shell technology columns, thirteen pain management opioids were resolved in 1.5 minutes; similarly eleven drugs of abuse (expanded SAMHSA) were resolved in 1.9 minutes. Both nearly identical methods exhibit one second wide peaks at base on a KinetexTM C18 2.6 ┬Ám 50 x 2.1 mm column. Sensitivity, reproducibility and productivity are dramatically improved after efficient sample cleanup and concentration using StrataTM X-C solid phase extraction (SPE). Both assays combine SPE and LC/MS/MS to provide improved throughput with significant reduction in analysis time, labor requirements, and cost per sample while increasing sensitivity, accuracy and precision.