Pitfalls in the Implementation of 25OH Vitamin D Assays by LC-MS

Tue 3:30 PM - Track 1: Vitamin D

Darryl Erik Palmer-Toy Kaiser Permanente

Darryl Palmer-Toy, Qibo Jiang, Gene Usher Kaiser Permanente, Southern California Regional Reference Laboratories

LC-MS is an attractive option for the accurate, sensitive, and specific measurement of 25-hydroxy vitamins D2 and D3 in human serum. However, the successful development and implementation of such a clinical assay presents many pitfalls to the unwary, as we can relate from personal experience. In this lecture, I will discuss nine specific questions: Sample collection Does phlebotomy tube affect sample stability? Preanalytic processing How to maximize recovery? Can we reduce labor and imprecision through automation? Assay standardization To what reference should we standardize? Chromatography Which interfering substances must be separated? Mass spectrometery Which masses should be monitored? Personnel Can your average CLS successfully perform this test?

Email: Darryl.E.Palmer-Toy@kp.org

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