|Prerequisite: Hands-on experience with GC-MS and/or LC-MS systems.|
Contrary to the belief of many, even ultra-high performance mass spectrometers cannot solve every clinical analysis problem; sometimes separation is the simple, effective and inexpensive, answer. Chromatographic separation is not rocket science; it is a science with a limited number of variables than can be optimized to improve separation prior to detection.
This 1-day course is designed for scientists with an interest in improving chromatographic analyses prior to detection by mass spectrometry. Discussions and exercises will focus on understanding the fundamental relationships in chromatographic peak resolution in order to explore options for improving separations. Practical considerations for varying experimental parameters will be discussed. Following the general discussion, specific considerations for both gas chromatography and liquid chromatography will be presented.
Bob Kobelski has taught chromatography and mass spectrometry courses for Hewlett-Packard and CDC where he currently is the program leader for the Laboratory Response Network – Chemical. Rayman Stanelle and Megan McGuigan are technology transfer chemists at the Centers for Disease Control and Prevention. They teach public health laboratories HPLC, GC, SPE and liquid chromatography tandem mass spectrometry emergency response methods for chemical threats, as well as courses in fundamentals, method development and troubleshooting.