MSACL Conference Schedule

Imaging Supported Membranes Beyond The Diffraction Limit
Mon 11:30 AM - Session: Tissue Imaging by Mass Spectrometry
Steven Boxer
During the past few years, our lab has developed a wide range of methods for patterning lipid bilayers on solid supports [Accts. Chem. Res. 35, 149 (2002); Curr. Opin. Chem. Biol., 11, 581(2007)]. These 2D fluids are interesting both as a model for biological membranes and as a physical system with unusual properties. Methods have been developed for controlling the composition of patterned membrane corrals by variations on microcontact printing and microfluidics. Charged components can be moved around within these fluid surfaces by a form of 2D electrophoresis. The planar geometry of supported bilayer systems is ideal for high resolution imaging methods. The lateral (x-y) composition of membranes can be analyzed by high spatial resolution secondary ion mass spectrometry (SIMS) using the NanoSIMS 50 (Cameca). Results will be described for simple membrane compositions, phase separated domains and tethered architectures suggesting the potential of this method for the analysis of membrane organization in complex membranes.
Email: [email protected]