MSACL Conference Schedule

A Global Peptide Profiling Approach To Identify Endogenous Peptide Substrate Interactions
Tue 4:30 PM - Session: Metabolomics
Alan Saghatelian
Enzymes are traditionally characterized using in vitro assays; however, these assays neglect important aspects of in vivo biology, including post-translational modifications, competing metabolic pathways, discrete localization, and the existence of unknown substrates. New approaches that enable the characterization of enzymes directly in their natural environments are of tremendous value for defining the biochemical role of an enzyme in vivo. Here, we describe the continued development and application of one such method, termed discovery metabolite profiling (DMP), for the identification of endogenous substrates of the biomedically relevant peptidases.