MSACL Conference Schedule

Rapid Blood Drug Screen Using Ultra Performance Liquid Chromatography - Time of Flight Mass Spectrometry (UPLC-TOF)
Wed 8:30 AM - Session: Toxicology
Scott Kriger
Commonly used methods for drug screening in clinical and forensic laboratories include immunoassays, GC/MS, and HPLC-UV/Vis. Limitations of these techniques include long analytical run times, inadequate sensitivity, high specimen volume requirements and high reagent costs.

A novel screening method for the simple and rapid identification of drugs of abuse and therapeutic drugs in whole blood, serum and plasma has been developed. This method provides a sensitive, specific, rapid and cost effective solution for screening over 300 drugs. The method includes a simple extraction limited to a protein precipitation with 1.0 mL of acetonitrile containing Proadifen as an internal standard.

Chromatographic separation is achieved using a Waters ACQUITY UPLC system employing a Waters ACQUITY UPLC HSS T3, 2.1 x 100 mm, 1.8