| MALDI-TOF MS has become a fast and reliable method for identification of microorganisms after cultivation. However, the method has not been widely applied to specimens taken from patients directly. In order to develop a rapid identification method, we analyzed bacteria collected from infected urine and cultured blood, directly. Bacteria were isolated via centrifugation from the urine or blood culture samples followed by washing with de-ionized water. Proteins were extracted by formic acid / acetonitrile. 1 |
