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| Addressing The Challenges of Throughput and Selectivity With High Resolution LC and New Scan Wave MS/MS Technology |
| Wed 2:30 PM - Session: Small Molecule Analytes |
| Robert Plumb |
| The accurate determination of the concentration of candidate pharmaceuticals or clinical markers of disease is essential for the development of new medicines and assessment disease state progression. Unfortunately having a validated assay in control plasma, serum or urine is not enough to assure success; nor is the selectivity of a MRM transition sufficient the variable in sample matrices due to phenotypic and genetic differences and effect of self-medication. These factors can make LC/MS/MS method development complicated and time consuming.
In this presentation we will discuss a strategy for evaluating LC/MS and sample preparation methodologies and sample matrices from different sources. In particular we will describe a methodology using high-resolution chromatography and mass spectrometry methodologies that allow for the simultaneous collection of MRM transitions for quantification and full scan MS for qualitative information. |
| Email: r.plumb@imperial.ac.uk |