MSACL Conference Schedule

Introduction to Mass Spectrometry
Sunday Only Short Course
Lead: Ken Busch
This 1-day course covers fundamental aspects of mass spectrometry, assuming an undergraduate level of basic chemistry, but no previous experience in mass spectrometric coursework or instrumentation. The following topics are covered: atomic and molecular masses, exact masses and isotopes, sample collection and handling, interfaces to chromatographic separations, ionization methods, mass analysis devices, ion detection, processing of mass spectral data, elementary spectral interpretation, considerations for mass spectrometry of large molecules, and an overview of resources for information and spectral interpretation.

Emphasis throughout this course is on basic analytical aspects of the methods discussed, especially GC/MS and LC/MS, with additional discussions that highlight recent applications of biological mass spectrometry using electrospray ionization and matrix-assisted laser desorption ionization. We also cover MS/MS, computer-assisted methods of data interpretation and presentation, and limits to mass spectrometric analyses.

The course handout includes a guide to printed and web resources, a list of acronyms used in the field of mass spectrometry, and a series of review questions used to test understanding. This introductory course is a logical precursor to other short courses that cover more specialized areas of mass spectrometry.

Prerequisite: Fundamentals of high school or college chemistry

Instructor: Kenneth L. Busch

Kenneth L. Busch completed twenty years of research in mass spectrometry before starting his current position at Wyvern. Highlights of his work include early work in secondary ion mass spectrometry, MS/MS, and coupling of planar electrophoresis and thin-layer chromatography with mass spectrometry, with 140 research publications and two patents. He has composed the tutorial column "Mass Spectrometry Forum" in Spectroscopy since 1994, and has taught the short course