Intact Protein Quantification by Mass Spectrometry
Wed 8:30 AM - Track 3: Novel Approaches
Mark W. Duncan
University of Colorado, Denver
View Long Abstract
There are few approaches for precise and sensitive protein quantification. Clinical chemists use immunoassays, but these require antibodies. These are not always available and their specificity may limit assay performance. Peptide-centric mass spectrometric approaches use sequence-specific enzymatic cleavage of proteins to peptides, and subsequent proteotypic peptide quantification. This eliminates the antibodies, but proteolysis can itself compromise assay specificity. Intact protein quantification by MALDI mass analysis is a third option; however, it must be combined with reproducible and efficient sample clean-up, ideally incorporating target protein concentration. The relative merits