Biomarkers of Tobacco Smoking-induced Oxidative Stress
Wed 12:00 PM - Track 3: Tobacco Exposure
Ian Blair
University of Pennsylvania
View Long Abstract
Cigarette smoke is composed of an extremely complex mixture that has long been known to cause oxidative stress. Smoking behavior has a significant effect on exposure to cigarette smoke and the toxic chemicals that are present. We have developed a liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS) method for quantifying urinary nicotine and 15 metabolites to assess tobacco smoke exposure. This has been coupled with LC-MRM/MS methods for urinary 7,8-dihydro-8-oxo-2’-deoxyguanosine, oxidized lipids and oxidized lipid glucuronide metabolites. The LC-MRM/MS methods we have been developed will be useful for determining whether oxidative stress plays a role in the etiology of lung cancer.