|Biomarkers of Tobacco Smoking-induced Oxidative Stress
|Wed 12:00 PM - Track 3: Tobacco Exposure
University of Pennsylvania
|View Long Abstract
|Cigarette smoke is composed of an extremely complex mixture that has long been known to cause oxidative stress. Smoking behavior has a significant effect on exposure to cigarette smoke and the toxic chemicals that are present. We have developed a liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS) method for quantifying urinary nicotine and 15 metabolites to assess tobacco smoke exposure. This has been coupled with LC-MRM/MS methods for urinary 7,8-dihydro-8-oxo-2’-deoxyguanosine, oxidized lipids and oxidized lipid glucuronide metabolites. The LC-MRM/MS methods we have been developed will be useful for determining whether oxidative stress plays a role in the etiology of lung cancer.