|Improving Measurement of Protein Biomarkers by Mass Spectrometry: Methods and Workflows|
Dr. Leigh Anderson, CEO of Plasma Proteome Institute
Measurement of protein biomarkers is most specifically achieved by quantifying proteotypic peptides by MRM-MS. The use of a new triple quadrupole mass spectrometry technology allows highly sensitive internally standardized measurements from digests of plasma. Further enhancement of both sensitivity and specificity by the use of anti-peptide antibodies to enrich peptides from the digest (SISCAPA) will be discussed. This approach will also be discussed in the context of potential automation considerations.
Quantitative Analysis of Underivatized 1,25-Dihydroxyvitamin D3 and D2 in Blood by UHPLC and Triple Quadrupole Mass Spectrometer Utilizing Ion Funnel Technology
Mr. Andre Szczesniewski, Senior Clinical Application Scientist, Agilent Technologies, Inc
Determination of 1,25-dihydoxyvitamin D3 levels in blood are very important as this metabolite of Vitamin D3 regulates blood calcium and phosphorus levels within the normal range. The liquid chromatography triple quadrupole mass spectrometry (LC-MS/MS) becomes an essential tool in the clinical laboratories due to its high sensitivity and specificity, excellent reproducibility, and it can be used in simultaneous analysis of multiple analytes. In this talk, we will discuss the development of a sensitive and reliable method for the quantitation of underivitized 1,25-dihydroxyvitamin D3 and D2 in blood using the LC-MS/MS utilizing Ultra High Performance Liquid Chromatography (UHPLC) and Triple Quadruple (QQQ) equipped with ion funnel technology.