11. A High-Throughput Mass Spectrometric Immunoassay for Intact Insulin, Insulin Metabolites, Synthetic Insulins, C-peptide, and C-peptide Metabolites for Population Proteomics Studies
Tue 12:00 PM - PosterSplash Track 2
Paul Oran
The Biodesign Institute at Arizona State University
View Long Abstract
Here we report on the development of a mulitplexed high-throughput mass spectrometric immunoassay (MSIA) that offers monoisotopic MALDI-TOF-MS detection of intact insulin, insulin metabolites (e.g. des B30), synthetic insulin analogs (e.g. Lantus), intact c-peptide, and c-peptide metabolites. Limits of quantification ranged from 15pM - 15nM, with coefficients of variation of < 10% for interday experiments (dynamic range = 1000, average R2 for three consecutive days of analysis = 0.99). Future studies will be designed using large populations of individuals (100s-1000s) with the goal to document the natural variation and quantification of insulin and c-peptide heterogeneity in the context of diabetes.