2. Increasing LC-MS Assay Robustness Through Increased Specificity Using High Resolution MRM-like Analysis
Tue 4:30 PM - PosterSplash Track 1
Christie Hunter
View Long Abstract
Interference in targeted quantitative assays can come from different sources and can degrade the quality of the quantitative data and the lower limit of quantitation achieved (LLOQ). When running MRM assays on human samples, it is imperative that assays are developed to be robust to the presence of potential and likely interferences. Recent QqTOF innovations have enabled a new acquisition strategy, where looped MS/MS spectra are collected at high resolution and then fragment ions are extracted to generate high resolution MRM-like data. Details and implications of this technique for increasing the sensitivity/specificity of MRM assays will be discussed.