|4. Fully Automated Analysis of PCR Products by High Performance ESI-TOF Mass Spectrometry: A Novel Platform for Clinical Research
|Tue 4:36 PM - PosterSplash Track 1
|Jose R. Gutierrez
|View Long Abstract
|High throughput electrospray ionization time-of-flight (ESI-TOF) mass spectrometric analysis of polymerase chain reaction (PCR) amplicons represents a novel and universal strategy for the detection and characterization of microorganisms associated with emerging infectious diseases. The process uses mass spectrometry, signal processing, and base composition analysis of PCR amplification products from biologically conserved regions of microbial genomes to simultaneously identify the organisms present in a specimen. In this work we describe an automated platform for the analysis of such products based on high throughput ESI-TOF mass spectrometry. The platform is capable of analyzing nearly 3000 PCR reactions in 24 hours.