2. Sensitive and Specific Quantitation of 1α, 25-Dihydroxy Vitamin D3 and Analogs using LC-MS/MS and Labeling Chemistry
Mon 4:30 PM - PosterSplash Track 1
Subhakar Dey
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Dehydration and inefficient ionization of 1α, 25-DHVD3 and its isomers/analogs in electrospray ionization mass spectrometers (ESI/MS), make them difficult to quantify by this method in a reliable manner. Apart from stability and ionizability a high resolution HPLC method is necessary to separate the isobaric isomers of 1α, 25-DHVD3. To address these challenges a novel reagent has been developed to derivatize 1α,25-DHVD3 and its analogs using a simple and short one step reaction. Detection limit for 1α, 25-DHVD3 in human serum was <5 pg/mL. Isobaric isomers of 1α, 25-DHVD3 were well resolved under chromatographic conditions addressing the interference issue.