18. Development and Validation of an LC-MS/MS Assay for Quantitation of Plasma Asymmetric Dimethylarginine (ADMA), Symmetric Dimethylarginine (SDMA), and Arginine
Mon 4:48 PM - PosterSplash Track 2
Alan Lueke
Mayo Clinic Rochester
View Long Abstract
The conversion of arginine to nitric oxide (NO) is catalyzed by nitric oxide synthase (NOS), and asymmetric dimethylarginine (ADMA) is an inhibitor of that enzyme. When NO synthesis is blocked, a number of biochemical processes are immediately affected, including vasodilation, platelet aggregation, monocyte adhesion, and the inflammatory pathway. Current methods for quantitation of ADMA include immunoassays, where cross-reactivity is problematic, or mass spectrophotometric methods which require manual sample preparation including solid phase extraction and/or derivitization. We developed a simple, rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method to measure ADMA directly in EDTA plasma.