30. Mass Spectrometry-based Analytical Methods for Adeno-associated Virus (AAV) Gene Therapy Vectors
Mon 4:54 PM - PosterSplash Track 3
Kim Van Vliet
University of Florida
View Long Abstract
There are 12 known Adeno-associated virus (AAV) serotypes and approximately 100 genetic variants. Diagnostic tests to validate the serotype or protein composition of AAV capsids are needed. Protein identification by mass spectrometry is a preferred analytical technique for AAV capsid serotype identification, particularly since antibody-based methods are unlikely to be able to distinguish highly homologous sequences or capsids that have been modified. These methods are also useful for identifying the presence of additional proteins that were not previously distinguishable using other methods. These additional proteins may represent novel virus host protein interactions or simply by products from vector manufacturing.