51. Vitamin D and Metabolites: Evaluation of Supported Liquid Extraction (SLE) Prior to UPLC-MS/MS Analysis
Poster: Mon 2:00-3:00PM
Lee Williams
Biotage GB Limited
View Long Abstract
Vitamin D is the collective term for a group of fat soluble pro-hormones which exist as two major forms, D2 (ergocalciferol) and D3 (cholecalciferol). Vitamin D is biologically inert within the body, however, two hydroxylation reactions provide the main active metabolites; 25-hydroxy-vitamin D and 1รก, 25-dihydroxy-vitamin D. The highly protein bound nature of vitamin D dictates that SPE assays require protein disruption prior to extraction. This poster investigates vitamin D extraction using 96-well supported liquid extraction (SLE) prior to UPLC-MS/MS analysis. Full results, discussion and conclusions will be shown in the poster.