| Vitamin D is the collective term for a group of fat soluble pro-hormones which exist as two major forms, D2 (ergocalciferol) and D3 (cholecalciferol). Vitamin D is biologically inert within the body, however, two hydroxylation reactions provide the main active metabolites; 25-hydroxy-vitamin D and 1รก, 25-dihydroxy-vitamin D. The highly protein bound nature of vitamin D dictates that SPE assays require protein disruption prior to extraction. This poster investigates vitamin D extraction using 96-well supported liquid extraction (SLE) prior to UPLC-MS/MS analysis. Full results, discussion and conclusions will be shown in the poster. |
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