MSACL 

52. 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 Analysis Using a QTRAP® 5500 Tandem Mass Spectrometer and the MPX™-2 High Throughput Multiplexed HPLC System
Poster: Mon 6:30-7:30PM
Jesse C. Seegmiller
AB SCIEX
View Long Abstract
Recently, low serum levels of 25-hydroxy vitamin D3 and D2 along with metabolites of vitamin D, have been linked to a number of conditions including; hypertension, autoimmune diseases, and cancer. This has driven significant increases in testing volume for these analytes at clinical laboratories. Accurate and rapid analysis of 25-hydroxyvitamin D is essential for assessing vitamin D status and determining its role in various diseases. Immunoassays have traditionally been used in clinical settings, but suffer from inherent methodological problems including decreased dynamic range and specificity. We present a sensitive, accurate, and robust high-throughput multiplexed assay for 25-hydroxyvitamin D using LC-MS/MS.
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