47. Development of a Candidate Reference Measurement Procedure for Determination of Phenytoin, Phenobarbital, Lamotrigine, and Topiramate in Human Serum Using Isotope-Dilution LC-MS/MS
Poster: Tue 2:00-3:00PM
Susan Tai
National Institute of Standards and Technology
View Long Abstract
Phenytoin, phenobarbital, lamotrigine, and topiramate are some of the most widely used antiepileptic drugs (AEDs). Monitoring of their concentrations in serum is important for the treatment of epilepsy. An isotope-dilution LC-tandem mass spectrometry reference measurement procedure (RMP) for the determination of the four AEDs in serum has been developed. This method involves SPE for sample clean-up and LC-MS/MS for measurement. Method accuracy with recoveries of added drugs ranged from 98.6% to 102.0%. The overall CVs were < 1%. This RMP demonstrating good accuracy and precision can be used to assess the accuracy of routine test methods.