|47. Development of a Candidate Reference Measurement Procedure for Determination of Phenytoin, Phenobarbital, Lamotrigine, and Topiramate in Human Serum Using Isotope-Dilution LC-MS/MS
|Poster: Tue 2:00-3:00PM
National Institute of Standards and Technology
|View Long Abstract
|Phenytoin, phenobarbital, lamotrigine, and topiramate are some of the most widely used antiepileptic drugs (AEDs). Monitoring of their concentrations in serum is important for the treatment of epilepsy. An isotope-dilution LC-tandem mass spectrometry reference measurement procedure (RMP) for the determination of the four AEDs in serum has been developed. This method involves SPE for sample clean-up and LC-MS/MS for measurement. Method accuracy with recoveries of added drugs ranged from 98.6% to 102.0%. The overall CVs were < 1%. This RMP demonstrating good accuracy and precision can be used to assess the accuracy of routine test methods.