50. A Simplified Workflow for the Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 using Commercially Available Plasma Calibrators and Controls and LC-MS/MS
Poster: Tue 6:30-7:30PM
Michael Jarvis
View Long Abstract
Liquid chromatography-tandem mass spectrometry is a powerful technique for the determination of 25-hydroxyvitamin D3 and D2 in serum and plasma samples, having the benefits of lower detection limits, greater specificity and a broader dynamic range than commercial immunoassays. This paper evaluates the use of commercially available whole blood calibrators and controls for the purpose of LC/MS/MS analysis. The off-line sample preparation is based on a simple pre-treatment step using a methanolic zinc sulphate precipitation reagent. This is followed by a quick and simple on-line sample clean-up and high performance liquid chromatography-tandem mass spectrometry to detect and quantify 25-hydroxyvitamin D.