55. Rapid Protein Extraction and Trypsin Digestion with Pressure Cycling Technology (PCT)
Poster: Mon 2:00-3:00PM
Paul H Pevsner
University of Missouri School of Medicine
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Pressure cycling technology (PCT) can extract protein from tissue and accelerate trypsin digest of proteins in minutes while other separation and digestion techniques can take hours. The Barocycler, a PCT instrument (Pressure BioSciences Inc., South Easton, MA) was used for these experiments. We hypothesized that proteins from any tissue could be rapidly extracted and trypsinized.