57. Sensitive LC-MS/MS Method for Determination of 25-Hydroxyvitamin D3 and its C3-Epimer after Diels-Alder Derivatization
Poster: Mon 2:00-3:00PM
Evgueni Fedorov
Warnex Bioanalytical Services
View Long Abstract
The small volume of blood samples obtained from infants under one year of age accentuates the requirement for a highly sensitive LC-MS/MS method for the quantification of 25-OHD3 and its C3-epimer. Derivatization of 25-OHD3 via Diels-Alder cycloaddition with a substituted triazolinedione resulted in enhanced sensitivity and an improvement in the chromatographic separation of the epimers. Complete separation of minor derivatized products was achieved in less than 6 minutes and allowed determination of vitamin D levels in infants using only 20 mcl of plasma.