76. Measurement of Urine Orotic Acid and Orotidine by UPLC-MS/MS
Poster: Mon 6:30-7:30PM
Lis Schwarz
ARUP Laboratories, Inc
View Long Abstract
Orotic acid and orotidine are intermediates in the synthesis of pyrimidine nucleotides. They can accumulate in disorders of the synthesis of pyrimidines, hereditary orotic aciduria, and in urea cycle defects. Their determination in urine is an essential tool to aid in the diagnosis of these inherited conditions. Here we report a new method to measure urine orotic acid and orotidine using stable isotope dilution with ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS).