| Protein turnover has been measured using amino acid stable isotope labeled tracers. The labeled tracer is incorporated into proteins, proteins of interest are isolated, hydrolyzed into their amino acid constituents, derivatized, and enrichment is measured via gas chromatography-mass spectrometry. This method has significant limitations including low throughput and the accuracy of this method can be compromised by the efficacy of the protein isolation step. We present an alternative method using targeted proteomics to measure protein fractional turnover rate of five proteins from tracheal aspirates obtained from premature babies using a primed continuous infusion of [5,5,5–2H3]leucine. |
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