Biomarker Discovery in Stroke Samples Using a Quantitative, Two-Pass LC-MS/MS Workflow Incorporating ROC Curve Analysis
Tue 11:00 AM - Track 2: Discovery Proteomics
Mary Lopez
View Long Abstract
Patent foramen ovale (PFO) is a highly prevalent, independent stroke risk factor in the general population. There is a lack of information on PFO-related neurovascular injury. We initiated a study using mass spectrometry to follow protein expression in PFO patients before and after surgical closure of the PFO. A Two-Pass, quantitative discovery workflow including application of ROC analysis and expression ratio providing efficient evaluation and scoring of putative biomarkers is described. The Two-Pass approach couples very accurate, full-scan quantification with MS/MS acquisition driven by an inclusion list generated from a ROC analysis of the full scan data.