| The characterization of fatty acids (FAs) is usually carried out after derivatization by GCMS or recently by LC-MS. We present a fast and sensitive orbitrap LC-MS assay after attaching a pyridine or secondary amine tag to FAs via an amide or ester linkage. This method resulted in detection limits in the low femtomole range, and the analytes, including biological relevant long chain saturated and unsaturated FAs, were analyzed in less than 15 minutes. Optimization of the assay using a variety of tags and advantages of orbitrap over tandem mass as well as applications including red blood cell analyses are discussed. |
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