Quantitative Reversed Phase LC/MS/MS Analysis of Ethyl Glucuronide and Ethyl Sulfate from Urine
The analysis of ethyl glucuronide (ETG) and ethyl sulfate (ETS), two metabolites of ethanol, in urine has surged in use as markers for evidence of alcohol consumption. This is primarily because ETG and ETS are detectable in the body for a longer period of time than ethanol, increasing the ability to determine alcohol consumption. Analytically the challenge is to achieve enough retention of these polar compounds to separate them from urine matrix interferences. This presentation will discuss a method for attaining accurate and reproducible quantitative results using traditional reverse phase conditions, minimizing the complexity of the method.
A Multiplexed Method for the Determination of Androgens in Serum and Plasma by HPLC-MS/MS
Androgens, naturally occurring sex hormones, are responsible for the development of the reproductive system and secondary sex characteristics. In this assay, three androgens are quantitated for clinical purposes: DHEA, Androstenedione, and Testosterone. To efficiently handle testing a large number of samples, a multiplexed method was developed using a Thermo Scientific TLX2 autosampler system and an API4000 triple quadrupole mass spectrometer (AB Sciex). To accommodate for pressure changes in the multiplexed systems, Phenomenex Kinetex columns were implemented.
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