79. Identification of Proteins in Plasma of Lung Cancer Patients and Control Groups Using the Combined LC-ESI-MS/MS Approach
Poster: Tue 2:00-3:00PM
Valery Shevchenko
N. N. Blokhin Russian Cancer Research Center
View Long Abstract
We used the LC-ESI-MS/MS methodology for bottom-up profiling blood plasma proteome.
809 proteins could be identified. From them: 53 increased expression level at transition from the control to squamous cell carcinomas I-II and III-IV stages; decrease in level of an expression observed for 45 of proteins;43 of proteins were not detected in control group; 92 of proteins were not detected in case group.
Our methodology is a practical approach to proteome mapping of blood plasma. Proteins whose expression levels logically changed from the control over IV stages SCC are potential markers for diagnostics of lung cancer.