Quantitative Analysis of IGF-1 by High Resolution Mass Spectrometry
Wed 9:00 AM - Track 2: Targeted Proteomics
Cory Bystrom
Quest Diagnostics Nichols Institute
View Long Abstract
Measurement of IGF-1 levels is useful in the diagnosis and treatment of growth hormone deficiency. Methodologies suitable for the clinical lab have been extensively developed over the last 30 years but criticisms remain regarding precision, accuracy, and inter-platform variability. We have developed an LC-MS method for the quantitative analysis of IGF-1 which makes use of high resolution, high mass accuracy measurement of intact IGF-1 from human serum. The approach provides excellent performance, excellent agreement to RIA, and freedom from interferences by IGF binding proteins without the use of a blocking step.