Dilute and Shoot: Comparison of Selected Reaction Monitoring with Full Scan MS/MS for Amphetamines, Opiates and Cocaine Metabolite

Wed 9:30 AM - Track 1: Toxicology

Robert Fitzgerald VA San Diego/UCSD

View Long Abstract

We developed a simplified LC/MS/MS procedure for quantifying and confirming drugs of abuse directly from urine specimens using selected reaction monitoring (SRM) and full scan product ion spectra. Deuterium labeled internal standards were added to urine which was analyzed using an ABI 4000 linear ion trap MS/MS. The ion trap provided about 100 fold increase in signal to noise as compared with the triple quadrupole for full scan product ion spectra while scanning at a faster rate.There was 100% concordance between the full scan identification and the SRM results for identification of amphetamine, methamphetamine, benzoylecgonine, and opiates.

Email: rlfitzgerald@vapop.ucsd.edu

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