A Reference Measurement Procedure to Quantify Urinary Albumin
Wed 9:30 AM - Track 2: Targeted Proteomics
Ashley S. Beasley
National Institute of Standards and Technology
View Long Abstract
Urinary excretion of albumin is a major diagnostic and prognostic marker of renal dysfunction and cardiovascular disease and accurate measurement of urinary albumin is vital to clinical diagnosis. Therefore, we have developed a MRM-based reference measurement procedure to quantify urinary albumin. Optimal MRM transitions were selected via tandem MS analysis of tryptic fragments of unlabeled HSA and 15N-rHSA on the basis of retention time reproducibility, peak intensity, and the degree of HSA sequence coverage. Pooled human urine was used to analyze the validity of the MS-based assay for urinary albumin quantitation.