Comparative Analysis of PCR Electrospray Ionization Mass Spectrometry to MALDI-ToF Mass Spectrometry for the Identification of Bacteria and Yeast from Positive Blood Culture Bottles
Wed 10:30 AM - Track 2: Microbes and Infectious Disease
Erin J. Kaleta
University of Arizona
View Long Abstract
The ability to rapidly and accurately detect bloodstream pathogens is the key objective for clinical diagnosis of bacteremia. New diagnostic methods utilizing mass spectrometry are emerging in the clinical field, using polymerase chain reaction mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry from positive blood culture bottles. These two methods were used to identify microorganisms from 154 positive blood culture bottles collected from patients with bacteremia, and the performance of these techniques were compared against standard clinical phenotypic testing. The accuracy of each method as well as pros and cons will be discussed during this presentation.