Strain Identification of Methicillin-Sensitive Staphylococcus Aureus from a Neonatal Intensive Care Unit using MALDI-ToF Mass Spectrometry
Wed 11:30 AM - Track 2: Microbes and Infectious Disease
Richard R. Drake
Eastern Virginia Medical School
View Long Abstract
Genus and species identification of bacteria using MALDI-TOF mass spectrometry is becoming standard in many clinical microbiology laboratories, yet methods for identification of strain differences within a species remains a challenge. To address this, primary clinical isolates of Staphylococcus aureus obtained from routine nasal swabs in a neonatal intensive care unit were evaluated by MALDI-TOF profiling. Emphasis was placed on distinguishing known methicillin sensitive and methicillin resistant S. aureus strains most frequently associated with blood infections. MALDI-TOF profiling could reproducibly differentiate laboratory MRSA and MSSA strains, but could not reliably be used with primary clinical isolates of MSSA.