Accelerating the Implementation of Mass Spectrometry in the Clinical Lab

MSACL

Current News in Clinical Mass Spectrometry

16 week(s) ago
New Fully Automated Sample Pretreatment System Enables Faster, Safer, and Simpler Biological LC-MS/MS Analysis
spectroscopyNOW
Tue Mar 7 12:07:06 2017
Shimadzu Scientific Instruments today announces the release of the Clinical Laboratory Automation Module (CLAM-2000), a fully integrated sample pretreatment module for LC-MS. The system automatically performs all of the processes necessary for analyzing blood and other biological samples, from scanning in information from the blood collection tubes to sample pretreatment and LCMS analysis. Automatic analysis is not only faster than manual analysis, it provides more precise results by reducing human error and improves laboratory safety and efficiency.The CLAM-2000 is the first truly integrated sample preparation system for LCMS that allows users to process samples in an instrument that is directly attached to the LCMS system, thus enabling a very simple and safe means for lab personnel to ... more

16 week(s) ago
Waters adds METLIN MS/MS Library to Progenesis Software, Extending Metabolomics Capabilities
spectroscopyNOW
Mon Mar 6 15:28:04 2017
Waters Corporation (NYSE: WAT) announces the Waters® METLIN® MS/MS Library for Progenesis® QI, which offers implementation of a METLIN library directly within Progenesis QI Software for use with Waters’ high resolution mass spectrometry (MS) systems. This library plug-in to Progenesis QI enhances metabolomics researchers’ ability to confidently identify metabolites in complex samples using mass spectrometry. METLIN is one of the leading comprehensive metabolomic databases used by researchers in the field.Progenesis QI Software enables researchers to analyze complex LC-MS data sets, visualize and extract reliable results, enabling users to rapidly identify and quantify significant changes between samples. The addition of direct access to the Waters METLIN MS/MS library streamlines ... more

18 week(s) ago
MALDI-TOF alone versus MALDI-TOF combined with real-time antimicrobial stewardship interventions on time to optimal antimicrobial therapy in patients with positive blood cultures
JCM Accepts
Wed Feb 22 12:28:32 2017
Introduction: Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) decreases time to organism identification and improves clinical and financial outcomes. The purpose of this study was to evaluate the impact of MALDI-TOF alone vs MALDI-TOF combined with real-time, pharmacist-driven, antimicrobial stewardship (AMS) intervention on patient outcomes. Methods: This single-center, pre-post quasi-experimental study evaluated hospitalized patients with positive blood cultures identified via MALDI-TOF combined with prospective AMS intervention, compared to a control cohort with MALDI-TOF identification without AMS intervention. AMS intervention included: real-time MALDI-TOF pharmacist notification, and prospective AMS provider feedback. The primary outcome was time to optimal ... more

21 week(s) ago
Milestone for the analysis of human proteomes
spectroscopyNOW
Wed Feb 1 04:56:05 2017
Munich, Germany. January 30, 2017Researchers led by the Technical University of Munich (TUM) report on the synthesis of a library of more than 330,000 reference peptides representing essentially all canonical proteins of the human proteome. It is a major milestone in the ProteomeTools project which aims at translating human proteome information into new molecular and digital tools with the potential for use in drug discovery, personalized medicine and life science research.In a manuscript published online in Nature Methods, ProteomeTools scientists report on the synthesis of a library of more than 330,000 reference peptides (termed PROPEL for ProteomeTools Peptide Library) representing essentially all canonical proteins of the human proteome. All peptides were analysed by multi-modal ... more

22 week(s) ago
Rapid identification of clinically relevant members of the genus Exophiala by MALDI-ToF mass spectrometry and description of two novel species: Exophiala campbellii and Exophiala lavatrina
JCM Accepts
Wed Jan 25 09:21:22 2017
Exophiala is a ubiquitous pleomorphic genus comprising at least forty species, many of which have been associated with superficial, visceral or systemic infections in humans, other mammals or cold-blooded animals. In this study we investigated the potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) for the identification of Exophiala species. A total of 89 isolates (including 50 human and 4 animal clinical isolates) stored in the National Collection of Pathogenic Fungi were identified by PCR amplification and sequencing of the internal transcribed spacer region 1. Eighty-three of the isolates corresponded to sixteen known species within Exophiala/Rhinocladiella. The remaining six isolates are shown by phylogenetic analyses based on four ... more

22 week(s) ago
SCIEX Launches New X500B QTOF Mass Spectrometry System for Standardised Biotherapeutic Characterisation
spectroscopyNOW
Wed Jan 25 07:35:07 2017
Newest X500 Mass Spec System is the first true benchtop, accurate mass system for simple, robust and rapid biologics characterisation during drug developmentSCIEX, a global leader in life science analytical technologies, today announced its latest solution in the X-Series Quadrupole Time of Flight (QTOF) mass spectrometry (MS) platform. The newest X-Series model, the X500B QTOF System, brings simplicity, high performance and robustness for biotherapeutic analyses to one of the most compact MS system footprints on the market. The new user-friendly SCIEX OS Software interface and powerful BioPharmaViewTM 2.0 processing software comprise an unmatched integrated LC-MS solution for mass spec experts, or novices, performing standard biotherapeutic characterisation.The X500R QTOF System emerged ... more

23 week(s) ago
Molecular and Matrix-Assisted Laser Desorption Ionization—Time of Flight Mass Spectrometry Characterization of Clinically Significant Melanized Fungi in India
JCM Accepts
Wed Jan 18 12:00:22 2017
Melanized or black fungi are a heterogeneous group of fungi causing cutaneous to systemic diseases with high mortality. These fungi are rarely reported as agent of human infections primarily due to difficulties in their classical identification. In this study we examined using molecular methods and MALDI-TOF MS the diversity of melanized fungi (MF) isolated from patients in 19 medical centers in India. Overall, during a 4-year period, 718 (5.3%) clinical specimens yielded MF. Of these, 72 (10%) isolates had clinical significance and were identified primarily by sequencing the ITS and LSU regions. MF represented 21 genera comprising 29 species, the majority of them belonging to the order Pleosporales (50%) and Chaetothyriales (22%). Among the 29 fungal species identified in this study only ... more

25 week(s) ago
Waters’ vitamin D analysis system receives CE mark
spectroscopyNOW
Wed Jan 4 12:10:04 2017
Waters Corporation has announced that its fully-validated Waters MassTrak Vitamin D Solution is now CE-marked in accordance with IVD Directive 98/79/EC for the quantitative measurement of 25(OH) D2 and D3 (25-OH-VitD) from human plasma and serum. The IVD LC-MS/MS system is a complete solution consisting of reagents and consumables, instrumentation, and software and support services for clinical laboratories routinely measuring vitamin D in patient samples. The Vitamin D solution is currently only available for sale in Europe.“Our MassTrak Vitamin D Solution is the first, CE-marked, single-vendor system to measure both vitamins 25(OH) D2 and D3 independently and accurately from human plasma and serum in a single analysis,” said Jose Castro-Perez, director of health sciences, global ... more

25 week(s) ago
Improved Differentiation of Streptococcus pneumoniae and Other S. mitis group Streptococci by MALDI Biotyper Using an Improved MALDI Biotyper Database Content and a Novel Result Interpretation Algorithm
JCM Accepts
Wed Jan 4 10:28:46 2017
Reliable distinction of Streptococcus pneumoniae and viridans group streptococci is important because of the different pathogenic properties of these organisms. Differentiation between S. pneumoniae and closely related S. mitis species group streptococci has always been challenging, even when using such modern methods as 16S rRNA gene sequencing or matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. In this study, a novel algorithm combined with an enhanced database was evaluated for the differentiation between S. pneumoniae and mitis species group streptococci. One hundred and one clinical mitis species group streptococcal strains and 188 clinical S. pneumoniae strains were identified both by the standard MALDI Biotyper database alone and combined ... more

27 week(s) ago
MALDI-TOF for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria
JCM Accepts
Wed Dec 21 08:56:28 2016
Species identification and drug susceptibility testing (DST) of mycobacteria is an important yet complex process, traditionally reserved for reference laboratories. Recent technical improvements in MALDI-TOF MS started to facilitate routine mycobacterial identifications in clinical laboratories. In this paper, we investigate the possibility to perform phenotypic MALDI-based DST in mycobacteriology, using the recently described MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA™). We randomly selected 72 clinical M. tuberculosis (MTB) and nontuberculous mycobacterial (NTM) strains, subjected them to MBT-ASTRA™ methodology and compared its results to current gold standard methods. Drug susceptibility was tested for rifampicin, isoniazid, linezolid, and ethambutol (MTB, ... more

28 week(s) ago
Characterization and diagnostic application of Trypanosoma cruzi trypomastigote excreted-secreted antigens shed in extracellular vesicles released from infected mammalian cells
JCM Accepts
Wed Dec 14 11:35:19 2016
Chagas disease, caused by Trypanosoma cruzi, although endemic in many parts of Central and South America, is emerging as a global health threat through the potential contamination of blood supplies. Consequently, in the absence of a gold standard assay for the diagnosis of Chagas additional antigens or strategies are needed. A proteomic analysis of the trypomastigote excreted secreted antigens (TESA) associated with exosomal vesicles shed by T. cruzi identified ∼80 parasite proteins; with the majority being trans-sialidases. Mass spectrometry analysis of immunoprecipitations performed using Chagas immune sera showed a marked enrichment in a subset of TESA proteins. Of particular relevance, for diagnostic applications were the retrotransposon hot spot (RHS) proteins, which are, absent in ... more

28 week(s) ago
Thermo Fisher launches immunotherapy program
spectroscopyNOW
Tue Dec 13 09:42:07 2016
Normal0falsefalsefalseEN-GBX-NONEX-NONE/* Style Definitions */table.MsoNormalTable{mso-style-name:"Table Normal";mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-noshow:yes;mso-style-priority:99;mso-style-parent:"";mso-padding-alt:0cm 5.4pt 0cm 5.4pt;mso-para-margin-top:0cm;mso-para-margin-right:0cm;mso-para-margin-bottom:8.0pt;mso-para-margin-left:0cm;line-height:107%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:EN-US;}Normal0falsefalsefalseEN-GBX-NONEX-NONE/* Style Definitions */table.MsoNormalTable{mso-style-name:"Table ... more

28 week(s) ago

PLoS One (p07)
Mon Dec 12 10:28:04 2016
We evaluated plasma samples HIV-infected individuals with different phenotypic profile among five HIV-infected elite controllers and five rapid progressors after recent HIV infection and one year later and from 10 individuals subjected to antiretroviral therapy, five of whom were immunological non-responders (INR), before and after one year of antiretroviral treatment compared to 175 samples from HIV-negative patients. A targeted quantitative tandem mass spectrometry metabolomics approach was used in order to determine plasma metabolomics biosignature that may relate to HIV infection, pace of HIV disease progression, and immunological response to treatment. Twenty-five unique metabolites were identified, including five metabolites that could distinguish rapid progressors and INRs at ... more

29 week(s) ago
Waters becomes founding partner of new phenome network
spectroscopyNOW
Mon Dec 5 08:49:07 2016
A consortium of leading research centers from six different countries has launched the International Phenome Centre Network (IPCN). Through its commitment to harmonized metabolic phenotyping methods, the IPCN will tackle some of the most pressing global health challenges, such as autism, cancer, diabetes, dementia and obesity. Supporting the network with its chromatography and mass spectrometry technologies and expertise, Waters is a founding corporate partner of the IPCN.Representing an advance in precision medicine research, metabolic phenotyping examines the dynamic interactions between our genes, environments, microbiomes, diets and lifestyles and their impact on disease. This research is made possible through advanced analytical science, including mass spectrometry.Waters is a world ... more

31 week(s) ago
Clinical Significance of Commensal Gram-Positive Rods Routinely Isolated from Patient Samples
Journal of Clinical Microbiology
Wed Nov 23 09:07:12 2016
Commensal bacteria from the skin and mucosal surfaces are routinely isolated from patient samples and considered contaminants. The majority of these isolates are catalase-positive Gram-positive rods from multiple genera routinely classified as diphtheroids. These organisms can be seen upon Gram staining of clinical specimens or can be isolated as the predominant or pure species in culture, raising a priori suspicion of a possible involvement in infection. With the development and adoption of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), suspicious isolates are now routinely identified to the species level. In this study, we performed a retrospective data review (2012 to 2015) and utilized site-specific laboratory criteria and chart reviews ... more

31 week(s) ago
Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium abscessus Subspecies According to Whole-Genome Sequencing
Journal of Clinical Microbiology
Wed Nov 23 09:07:08 2016
This study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization–time of flight mass spectrometry with the Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. A total of 175 clinical M. abscessus strains were identified by whole-genome sequencing analysis: 139 Mycobacterium abscessus subsp. abscessus and 36 Mycobacterium abscessus subsp. massiliense. The research-use-only (RUO) Saramis Knowledge Base database v.4.12 was modified accordingly by adding 40 M. abscessus subsp. abscessus and 19 M. abscessus subsp. massiliense reference spectra to construct subspecies SuperSpectra. A blind test, used to validate the remaining 116 isolates, yielded 99.1% (n = 115) reliability and only ... more

33 week(s) ago
Relative prevalence and antimicrobial susceptibility of clinical isolates of Elizabethkingia species based on 16S rRNA gene sequencing
JCM Accepts
Wed Nov 9 11:07:31 2016
Some of the previously reported clinical isolates of E. meningoseptica could be later named species of Elizabethkingia. We determined the accuracy of species identification (with two MALDI-TOF MS and the VITEK 2 GN card), relative prevalence of three Elizabethkingia spp. in clinical specimens, and antimicrobial susceptibility of the species identified by 16S rRNA gene sequencing. Specimens for culture were collected from patients in a university hospital in Korea between 2009 and 2015. All 3 Elizabethkingia spp. were detected in patients: among the 86 isolates identified by 16S rRNA gene sequencing, 17 (19.8%) were E. meningoseptica, 18 (20.9%) were E. miricola, and 51 (59.3%) were E. anophelis. Only the MALDI-TOF VITEK MS system with amended data base correctly identified all of the ... more

34 week(s) ago
Agilent signs agreement with RapidFire MS developers
spectroscopyNOW
Thu Nov 3 09:42:07 2016
Normal0falsefalsefalseEN-GBX-NONEX-NONE/* Style Definitions */table.MsoNormalTable{mso-style-name:"Table Normal";mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-noshow:yes;mso-style-priority:99;mso-style-parent:"";mso-padding-alt:0cm 5.4pt 0cm 5.4pt;mso-para-margin-top:0cm;mso-para-margin-right:0cm;mso-para-margin-bottom:8.0pt;mso-para-margin-left:0cm;line-height:107%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:EN-US;}Agilent Technologies and PureHoney Technologies have announced a co-marketing agreement to develop new ... more

35 week(s) ago
Informing Antibiotic Treatment Decisions: Evaluating Rapid Molecular Diagnostics (RMDs) to Identify Susceptibility and Resistance to Carbapenems against Acinetobacter spp. PRIMERS III
JCM Accepts
Wed Oct 26 10:36:39 2016
The widespread dissemination of carbapenem-resistant Acinetobacter spp. has created significant therapeutic challenges. At present, rapid molecular diagnostics (RMDs) that can identify this phenotype are not commercially available. Two RMD platforms, polymerase chain reaction combined with electrospray ionization mass spectrometry (PCR/ESI-MS) and molecular beacons (MB), for detecting genes conferring resistance/susceptibility to carbapenems in Acinetobacter spp. were evaluated. An archived collection of 200 clinical Acinetobacter spp. isolates was tested. Predictive values for susceptibility and resistance were estimated as a function of susceptibility prevalence, and were based on the absence or presence of beta-lactamase (bla) NDM, VIM, IMP, KPC, and OXA carbapenemase genes (e.g., ... more

36 week(s) ago
Thermo Fisher joins Cancer Moonshot
spectroscopyNOW
Wed Oct 19 11:28:05 2016
Thermo Fisher Scientific has joined the Cancer Moonshot, an initiative led by US Vice President Joe Biden aimed at making more therapies available to more patients while also improving cancer detection at early stages and, ultimately, preventing the disease. Initially, Thermo Fisher will join a liquid biopsy-based precision oncology pilot, called the Blood Profiling Atlas, which is designed to make it easier for oncologists to access databases of liquid biopsy information when diagnosing patients.“The Cancer Moonshot is an ambitious, all-hands initiative that requires the best minds and best technologies working collaboratively,” said Marc Casper, president and chief executive officer of Thermo Fisher Scientific. “As part of our broader focus on precision medicine, which includes ... more