MSACL 2018 EU Program

MSACL 2018 EU: Congress Program

Salzburg, Austria • September 9 - 13, 2018

With Thanks to Our Corporate Congress Sponsors:
Thermo   Shimadzu
Agilent   Chromsystems
And Special Thanks to Our Travel Grant Sponsors:
Thermo


= Emerging. More than 5 years before clinical availability.
= Expected to be clinically available in 1 to 4 years.
= Clinically available now.

Sunday

Sunday
1100
1400
Lunch on Own
@ Your Choice

Some nearby (within one block) recommendations include:
1. The Heart of Joy (Vegetarian and Vegan)
2. my Indigo Kongresshaus (Asian Fusion)
3. Cafe Fingerlos (Cafe with the best breakfast in town)
4. IMLAUER Sky (Rooftop patio with a view)
5. Cafe Wernbacher (Austrian, great interior for a rainy day with much of the original 1950s decor, open 9am-midnight daily with Sunday Brunch buffet until 2pm)

A little further afield:
1. Cafe Sacher (for a patio on the river)
2. Barenwirt (Cozy and Traditional Austrian, fills up so arrive early)
3. Antichi Sapori (Tasty Italian food and pizza in casual family run style, Open 11:30-2:00pm and 5:30-9:00pm, Closed Wed and Thurs)

576
Sunday
1100
1900
Badge Pickup
@ Entrance Foyer
574
1400
1800

Short Course


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COURSE START
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & William Slade, PhD
Level: Beginner - Intermediate
@ Mozart 5
COURSE START
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Randall Julian, PhD
Level: Intermediate
@ Mozart 4
COURSE START
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: Beginner
@ Mozart 2
COURSE START
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Erik J. Soderblom, PhD
Level: Intermediate
@ Paracelsus Hall
COURSE START
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: Advanced
@ Papageno Hall
COURSE START
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: Beginner to Intermediate
@ Doppler Hall
COURSE START
Proteomics 201
Clinical Proteomics
Cory Bystrom, PhD & Chris Shuford, PhD
Level: Intermediate - Advanced
@ Trakl Hall
Sunday
Every 0:50
for 10 min
Coffee Break
@ Entrance Foyer

888
Sunday
1800
1830
Petite Reception
@ Entrance Foyer

A quick touch of wine and cheese before exploring the city for dinner.
578
Sunday
1800
Dinner on Own
@ Your Choice

Dinner Suggestions:
1. Gablerbrau (Historic ambiance with traditional food)
2. Augustiner Braustubl (Historic Abby turned beer garden with food stalls)
3. Antichi Sapori (Tasty Italian food and pizza in casual family run style, open 11:30-2:00pm and 5:30-9:00pm, closed Wed and Thurs)
4. Cafe Wernbacher (Austrian, great interior for a rainy day with much of the original 1950s decor, open 9am-midnight daily)

After dinner:
1. Murphys Law Irish Pub

579
Sunday
1830
2100
SciCom Discussion Group
@ 1st Floor Cafe

Private Meeting for Scientific Committee members; includes dinner
577

Monday

Monday
800
900
Welcome Coffee
@ Entrance Foyer

Enjoy coffee, a pastry and a chat with colleagues before the day starts.
580
900
1300

Short Course


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Continued from Sunday
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & William Slade, PhD
Level: Beginner - Intermediate
@ Mozart 5
Continued from Sunday
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Randall Julian, PhD
Level: Intermediate
@ Mozart 4
Continued from Sunday
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: Beginner
@ Mozart 2
Continued from Sunday
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Erik J. Soderblom, PhD
Level: Intermediate
@ Paracelsus Hall
Continued from Sunday
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: Advanced
@ Papageno Hall
Continued from Sunday
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: Beginner to Intermediate
@ Doppler Hall
Continued from Sunday
Proteomics 201
Clinical Proteomics
Cory Bystrom, PhD & Chris Shuford, PhD
Level: Intermediate - Advanced
@ Trakl Hall
Monday
1300
1400
Lunch
@ Entrance Foyer

Lunch provided for short course attendees.
589
1400
1800

Short Course


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COURSE START
Lab Medicine 101
Basics of Laboratory Medicine
Prof. Dr. med. Michael Vogeser
Level: Beginner
@ Mozart 3
COURSE START
Proteomic Microbiology 201
Bottom-Up and Top-Down Proteomic Approaches for Bacterial Identification and Characterization, a Focus on MALDI-TOF and Advanced Technologies
Jean Armengaud, PhD, Stefan Zimmermann, MD
Level: Beginner - Intermediate
@ Trapp Zimmer
Continued from Monday AM
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & William Slade, PhD
Level: Beginner - Intermediate
@ Mozart 5
Continued from Monday AM
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Randall Julian, PhD
Level: Intermediate
@ Mozart 4
Continued from Monday AM
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: Beginner
@ Mozart 2
Continued from Monday AM
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Erik J. Soderblom, PhD
Level: Intermediate
@ Paracelsus Hall
Continued from Monday AM
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: Advanced
@ Papageno Hall
Continued from Monday AM
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: Beginner to Intermediate
@ Doppler Hall
Continued from Monday AM
Proteomics 201
Clinical Proteomics
Cory Bystrom, PhD & Chris Shuford, PhD
Level: Intermediate - Advanced
@ Trakl Hall
Monday
1800
1830
Petite Reception
@ Entrance Foyer

A quick touch of wine and cheese before exploring the city for dinner.
599
Monday
1800
Dinner on Own
@ Your Choice

Dinner suggestions:
1. Zum fidelen Affen (best patio dinner on a nice evening, closed Sunday)
2. Die Wiesse (Brewery Pub with beer garden open till 2am Mon-Sat)
3. Gablerbrau (Historic ambiance with traditional food)
4. Augustiner Braustubl (Historic Abby turned beer garden with food stalls)
5. Antichi Sapori (Tasty Italian food and pizza in casual family run style, open 5:30-9:00pm, closed Wed and Thurs)
6. Cafe Wernbacher (Austrian, great interior for a rainy day with much of the original 1950s decor, open 9am-midnight daily)

After dinner:
1. Murphys Law Irish Pub

600

Tuesday

Tuesday
800
1600
Place Posters
@ 1st Floor Exhibit Hall

ALL posters should be placed by 16:00.
601
Tuesday
800
900
Welcome Coffee
@ Entrance Foyer

Enjoy coffee, a pastry and a chat with colleagues before the day starts.
602
900
1230

Short Course


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Continued from Monday PM
Lab Medicine 101
Basics of Laboratory Medicine
Prof. Dr. med. Michael Vogeser
Level: Beginner
@ Mozart 3
Continued from Monday PM
Proteomic Microbiology 201
Bottom-Up and Top-Down Proteomic Approaches for Bacterial Identification and Characterization, a Focus on MALDI-TOF and Advanced Technologies
Jean Armengaud, PhD, Stefan Zimmermann, MD
Level: Beginner - Intermediate
@ Trapp Zimmer
Continued from Monday PM
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & William Slade, PhD
Level: Beginner - Intermediate
@ Mozart 5
Continued from Monday PM
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Randall Julian, PhD
Level: Intermediate
@ Mozart 4
Continued from Monday PM
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD & Grace van der Gugten
Level: Beginner
@ Mozart 2
Continued from Monday PM
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Erik J. Soderblom, PhD
Level: Intermediate
@ Paracelsus Hall
Continued from Monday PM
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Russell Grant, PhD & Brian Rappold
Level: Advanced
@ Papageno Hall
Continued from Monday PM
Metabolomics 202
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Level: Beginner to Intermediate
@ Doppler Hall
Continued from Monday PM
Proteomics 201
Clinical Proteomics
Cory Bystrom, PhD & Chris Shuford, PhD
Level: Intermediate - Advanced
@ Trakl Hall
Tuesday
1230
CONGRESS OPEN
@ Europa Hall
628
Tuesday
1230
1400
Opening Lunch
@ Europa Hall
614
Tuesday
1330
1345
Welcome, Introduction & Orientation
@ Europa Hall
615
Tuesday
1345
1400
CMS Journal Overview
@ Europa Hall

An overview on the current state of the journal, how to subscribe, the path to an Impact Factor, citations, scope, desired article format, webinars.
616

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1400

Opening Plenary Lecture


@ Europa Hall
Chair: Oleg Mayboroda

1400
1445
Metabolomics Messages on Human Health and Disease
Jurek Adamski
Helmholtz Zentrum M

The human metabolome represents functional read out of processes in health and disease. The metabolome is both stable and highly dynamic. Stable components are determined by genetics, genomic imprinting or physiological homeostasis. The dynamics originates from circadian rhythm, hormonal status, nutrition, environmental exposure, ageing, medication or disease. Despite its interlaced origin metabolome specifically reflects distinct processes. Metabolomics studies request therefore a special study design. Unique metabolomics signatures have been identified pre-disease (like type 2 diabetes), in disease progression (chronic kidney disease) or in companion diagnostics (drug action monitoring). Metabolomics informed diagnostics has great potential for selectivity, specificity and multiplexing of indications to be screened for.

Tuesday
1445
1500
Coffee Break
@ Europa Foyer
618
1500
1600

State of the Science Address
@ Europa Hall

Presented by MSACL EU Scientific Committee Topic Leads, this address will provide an overview of the applications and technologies currently being used in Clinical Labs, and a clear view of the development pipeline. It will highlight applications expected to be available in the near-future, as well as emerging applications, and key contributors. Relevant talks, posters, and people present at the congress will be identified, enabling you to optimize your learning path and more effectively target potential network connections. Whether you are new to Clinical Mass Spectrometry, or a seasoned veteran, the State of the Science address should be on your agenda.

Brian Keevil (Endocrinology)
Manfred Wuhrer (Glycomics)
Oleg Mayboroda (Metabolomics)
Jean Armengaud (Microbiology)
Christa Cobbaert (Proteomics)
Michael Vogeser (Small Molecules)
Tiffany Porta (Tissue Imaging)

Tuesday
1600
1630
Coffee Break
@ Europa Foyer
620

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Tuesday
1630
1700
Poster Lightning Talks
@ Europa Hall

15-20 poster presenters will be selected to present their pitch from the plenary podium within 90 seconds and with 1 slide (PDF format).
622

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Tuesday
1700
1730
Exhibitor Lightning Talks
@ Europa Hall

15-20 exhibitors will be selected to present their pitch from the plenary podium within 90 seconds and with 1 slide (PDF format).
621
Tuesday
1730
EXHIBITS OPEN
@ 1st Floor Exhibit Hall
624
Tuesday
1730
2000
Exhibitor Reception
@ 1st Floor Exhibit Hall

Buffet dinner served.
623

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Tuesday
1800
1900
MEET-THE-EXPERTS : Booth Tours
@ 1st Floor Exhibit Hall

Join an expert as they tour the booths and learn more about technologies available, what experts are looking for during the exchange, and how they interact with the booth vendors. Increase your network and your knowledge of products and services available.
625

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1900
2000
Troubleshooting Poster Rounds : Part I
@ 1st Floor Exhibit Hall

19:00 @ poster #25a
What Happened to My Topiramate?
Magdalena Rajska
SPADIA Lab, a.s.
19:15 @ poster #25b
Optimizing Chromatographic Conditions for HILIC Analysis of Catecholamines
Gareth Hammond
Waters Corporation
19:30 @ poster #25c
An Alternative Solution to High Blank Problems
Anne Schmedes
Department of Clinical Biochemistry
2000
2200
Discussion Group
@ Mozart 4-5
Tuesday
2200
Enjoy the City
@ Your Choice

or get a good night's sleep
627

Wednesday

Wednesday
745
900
Welcome Coffee
@ Entrance Foyer

Enjoy coffee, a pastry and a chat with colleagues before the day starts.
603
800
845

Corporate Workshop(s)


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Thermo Scientific
@ Papageno (Track 3)

Pre-Register

Targeted Screening and Semi-Quantitation of Drugs in Plasma using High-Resolution Accurate-Mass Detection and Online Sample Preparation

Sensitive and selective analytical methods are required in clinical research and forensic toxicology to properly identify a broad range of analytes in complex matrices. The existing methods need to be constantly updated as new compounds, such as designer drugs, become readily available to the illicit drug market. Liquid chromatography coupled to mass spectrometry (LC-MS) has been widely used in this area for years. So far, the preferred detection technology has been triple quadrupole MS for its selectivity in selected reaction monitoring (SRM) mode. However, this approach suffers from the limitation of being able to perform only targeted analysis, while not being useful for unknown screening of new drugs.

Come to our educational seminar to learn more.

For Research Use Only. Not for use in diagnostic procedures.

SCIEX
@ Paracelsus (Track 4)

Pre-Register

A Clinical Method for Determination of Anti-Infectious Drugs in Serum
Anders Blomgren
Associate professor at Division of Clinical Chemistry and Pharmacology, Lund University

The initiative for this work was taken by physicians from four different disciplines; intensive care, clinics for infection, thorax and transplantation. There has been an unmet need for these types of analysis and especially with short response times. A TDM wish list was produced that containing about 25 different anti-infection drugs and we agreed on starting to develop methods for 7 of them as a first step.
During this talk the following will be presented.
• Introduction
• Analytes – chemistry/properties/stability
• LC, MS and Sample prep development and problem solving
• Validation with results
• Conclusion and future

Wednesday
845
900
Intermission
@ Entrance Foyer
633

Scientific Session 1


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Track 1
Mozart 1-3

Lipidomics
Chair: Julijana Ivanisevic

Track 2
Mozart 4-5

Disease Implications and Diagnosis
Chair: Grace van der Gugten

Track 3
Papageno

Proteomics Keynote
Chair: Christa Cobbaert

Track 4
Paracelsus

New Technologies
Chair: Vladimir Frankevich

Track 5
Trakl

Endocrinology
Chair: Flaminia Fanelli

Track 6
Doppler

Practical Training : Proteomic Assay Development
Chair: Tim Collier

900
920
Defining Boundaries of Lipidomic Variations in Human Cohorts -
Federico Torta
National University of Singapore
Simultaneous and Automated LC-MS Analysis of Plasma Methylmalonic Acid and Short Chain Fatty Acids Using a Carbodiimide-based Derivatization -
Mark Pratt
University Medical Center Groningen
Clinical Proteomics: The Path Towards Implementation -
Harald Mischak
mosaiques therapeutics GmbH & University of Glasgow
Probing Amyloid Aggregation Dynamics in vivo, Using SILK Imaging (iSILK) -
Jörg Hanrieder
University of Gothenburg
Evaluation of Age and Metabolic Status Impact on Serum Steroid Hormones and Product/Precursor Ratios for the Generation of LC-MS/MS Reference Interval in Men -
Marco Mezzullo
University of Bologna
Proteomic Assay Development: Digest Optimization and Quality Control -
Timothy Collier
Quest Diagnostics - Cleveland HeartLab
920
940
Shotgun Lipidomics of Cerebrospinal Fluid in Neurological Disorders -
Gábor Balogh
Biological Research Centre, Hungarian Academy of Sciences
Mitochondrial Proteins as Parkinson’s Disease Circulatory Biomarkers – a Translational Study -
Sandra Anjo
University of Coimbra (CNC-UC)
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Keynote, continued
...
Identifying Biomarkers in FFPE Breast TMAs Using DESI-MSI -
Ólöf Gerdur Ísberg
University of Iceland
Quantification of Testosterone, Androstenedione and 17-Hydroxyprogesterone Collected Using Mitra Micro Sampling Devices -
David Marshall
Manchester University NHS Foundation Trust
...
Extended Session
...
940
1000
Understanding the Interplay Between Inflammatory Lipid Signaling and Demyelination Process: A Lipidomics Approach Applied to 3D Human Brain Model -
Maria Laura Santoru
University of Cagliari / University of Lausanne
The Singapore Lipidomics Incubator: A Model for Engagement and Translation -
Anne Bendt
National University of Singapore
...
Keynote, continued
...
MALDI Imaging Through Laser-Induced Post-Ionization Pushes the Boundaries of Pharmaceutical Development Studies -
Florian Barré
M4I, Maastricht University
Direct Analysis - No Sample Preparation - of Bioavailable Cortisol in Human Plasma by Weak Affinity Chromatography (WAC; Affinity LC/MS) -
Sten Ohlson
Nanyang Technological University
...
Extended Session
...

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Wednesday
1000
1100
Poster Session 1
@ 1st Floor Exhibit Hall

Selected posters to be attended for 1 hour. Refer to program for posters attended during this period.
692

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Wednesday
1000
1100
MEET-THE-EXPERTS : Poster Tours
@ 1st Floor Exhibit Hall
703

Scientific Session 2


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Track 1
Mozart 1-3

Metabolomics Methodology
Chair: Elizabeth Want

Track 2
Mozart 4-5

All About Breath Analysis
Chair: Neus Fabregat-Cabello

Track 3
Papageno

Endocrine Keynote
Chair: Brian Keevil

Track 4
Paracelsus

Glycomics 1
Chair: Guinevere Lageveen-Kammeijer

Track 5
Trakl

MS/MS for Proteotyping Pathogens
Chair: Simon Cameron

Track 6
Doppler

Practical Training : Calibrators and Internal Standards for Protein MS
Chair: Chris Shuford

1100
1120
New Insights in CE-MS-based Metabolomics -
Nicolas Drouin
University of Geneva
Mass Spectrometry Applied to Large-Scale Multi-Centre Clinical Project in Breath Research -
Ilaria Belluomo
Imperial College London
Mass Spectrometry Harmonisation: Paediatric Steroid Tales -
Ronda Greaves
Murdoch Children's Research Institute
Global Glycoproteomic Analysis of O-glycosylation in Human Herpesviruses -
Ieva Bagdonaite
University of Copenhagen
Phylopeptidomics: A Step Closer to Adopting the Microbiome Data into Clinical Practice -
Lucia Grenga
CEA
Calibrators and Internal Standards in Protein Mass Spectrometry Assays: Differences, Commonalities, and Best-Practice -
Christopher Shuford
Laboratory Corporation of America
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View Slides (PDF)

1120
1140
Combined Targeted and Untargeted Strategy for Quantification of Amino Acids and Acylcarnitines in Clinically Relevant Biofluids and Tissue Lysates -
Vera van der Velpen
Metabolomics Unit, University of Lausanne
Cross Platform Validation of Mass Spectrometry for the Diagnosis of Colorectal Cancer Using a Breath Test -
Georgia Woodfield
Imperial College London
...
Keynote, continued
...
In Depth O-Glycosylation Structural Analysis of Colorectal Cancer Cell Lines -
Katarina Madunic
Leiden University Medical Center
Characterization of the Virulome of Staphylococcus Aureus by a Highly Multiplex Approach with Scout-MRM -
Mouton Nicolas
University de Lyon Claude Bernard
...
Extended Session
...
1140
1200
Development of a Pipeline for Automated Reconstruction and Annotation of Data Independent Acquisition LC-MS Datasets -
Gonçalo Graça
Imperial College London
A Pilot Study Profiling Volatile Organic Compounds (VOCs) in the Breath of Patients with CRC Using Proton Transfer Mass Spectrometry -
Harrypal Panesar
Imperial College London
...
Keynote, continued
...
HT UPLC Profiling of Total Plasma N-Glycans in Type II Diabetes Mellitus from Patients and Healthy Individuals in a Ghanaian Population -
Elham Memarian
Leiden University medical centre
Deciphering MALDI-TOF Identification of Bacteria Using a Proteogenomic Approach -
Frédéric Jauffrit
Université de Lyon
...
Extended Session
...
Wednesday
1200
1330
Lunch
@ 1st Floor Exhibit Hall

Lunch to be provided in the Exhibit Hall. Get ready to join a Corporate Workshop after lunch.
672

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1230
1330
Troubleshooting Poster Rounds : Part II
@ 1st Floor Exhibit Hall

12:30 @ poster #26a
Metformin Interference in LC-MS/MS Analysis of Plasma Methoxycatecholamines
Marianne Bergmann
Lillebaelt Hospital, Vejle
12:45 @ poster #26b
Charge Wars of Ion Suppression - Awakening the Force for the Analysis of Estrogens in Clinical Research
Robert Wardle
Waters Corporation
13:00 @ poster #26c
Cortisol Everywhere!
Neus Fabregat-Cabello
University of Liège, CHU de Liège
13:15 @ poster #26d
Deuterium or 13C-labeled internal standards for quantification of methylmalonic acid in serum?
Morten Trelle
Department of Clinical Biochemistry, OUH Svendborg
1330
1415

Corporate Workshop(s)


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Shimadzu
@ Mozart 1-3 (Track 1)

Pre-Register

Better Ways of Working in Routine Clinical Pathology Laboratories

Healthcare is dynamic and knows no bounds and so routine clinical pathology laboratories face increasing pressures on cost, time and delivering actionable data.

At Shimadzu, we believe there's always a way to make life better. This seminar explores a few options to make a difference.

1) Neil LOFTUS - SHIMADZU Corporation, UK
Expanding the capability of mass spectrometry in routine clinical pathology – creating better ways of delivering actionable data.

2) Andrea Radeljak, Matea Ivić – KB Merkur, Croatia
Automating standardized workflows in the clinical laboratory – using the Shimadzu CLAM-2000 integrated with LC-MS/MS to help increase sample throughput and make it easier for users.

3) Sigrid BAUMGARTEN - Alsachim SAS, France
From core expertise of Stable Isotope Labelled Compounds to reagent kits for high quality LCMS data

Disclaimer: For Research Use Only. Not for use in diagnostic procedures. Not available in the USA, Canada and China.

Thermo Scientific
@ Mozart 4-5 (Track 2)

Pre-Register

Fully Automated LC-MS – Theory becomes Reality

The use of LC-MS in diagnostic pathology has been limited by the complexity of equipment, materials and skills required to establish a fully validated service. A review showed only 19% of respondents were running VitaminD using LC-MS. On the basis that this is the gold standard, a fully validated and simple-to-use mass spectrometer has been a long awaited development. This presentation shares the first practical experience of using the Thermo Scientific™ Cascadion™ SM Clinical Analyser, in a laboratory, where staff have no prior experience of using LC-MS technology.

Automated LC-MS/MS: A disruptive technology will improve the quality of care for our patients
The LC-MS/MS platform provides solutions to complex analytical problems for clinical routine laboratories. This development started decades ago and the technology has proven to deliver reliable results with unreached specificity in TDM, DoA testing, endocrine diagnostics and newborn screening. In this presentation the concept of a fully integrated and automated LC-MS/MS is described. Special focus is on features of the Thermo Scientific™ Cascadion™ SM Clinical Analyzer that can overcome the limitations of open systems.

Product IVD/CE-marked. Product not 510(k) cleared and not yet available for sale in the US.

Agilent Technologies
@ Paracelsus (Track 4)

Pre-Register

Developing and Delivering Advanced Protein Biomarker Tests for an Era of Precision Medicine
Speaker: Prof. Stephen R Pennington, Senior Fellow UCD Conway Institute, University College Dublin, Ireland

This presentation will address the key issues around the development of protein signatures in areas of significant unmet clinical need and potential strategies for the delivery and implementation of multiplexed protein biomarkers in a diagnostic setting.

View abstract

For Research Use Only. Not for use in diagnostic procedures.

Waters
@ Trakl (Track 5)

Pre-Register

Determination of Steroid Hormones in biological matrices by Tandem Mass Spectrometry

The determination of steroid hormones in biological matrices requires high analytical sensitivity and is particularly challenging due to analytical interferences from structurally related steroid hormones and synthetic derivatives. LC-MS/MS can provide the analytical sensitivity, selectivity and precision required to enable accurate quantification of organic compounds in human biological liquid matrices.

As a leading clinical LC-MS/MS solutions partner, Waters is a trusted provider of in vitro diagnostic medical devices, consumables, informatics, and support services for high performance LC-MS/MS systems.

Join us at this workshop to gain insights into how our customers have addressed the challenging analysis of steroid hormones by implementing Waters solutions.

LC-MS/MS Analysis of Steroids in the Clinical Laboratory
Dr. Brian Keevil, University Hospital of South Manchester , Biochemistry Department

Download Dr. Keevil's abstract

For Research Use Only. Not for use in diagnostic procedures.

Learn more on Waters Solutions for LC/MS Analysis

Wednesday
1415
1430
Intermission
@ Entrance Foyer
701

Scientific Session 3


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Track 1
Mozart 1-3

Metabolic Markers
Chair: Luke Whiley

Track 2
Mozart 4-5

Small Molecule Keynote
Chair: Michael Vogeser

Track 3
Papageno

Cardiovascular and Thrombotic Disease
Chair: Tim Collier

Track 4
Paracelsus

Tissue Imaging Keynote
Chair: Tiffany Porta Siegel

Track 5
Trakl

Glycomics 2
Chair: TBA

Track 6
Doppler

Practical Training : EU Regulations
Chair: Brian Keevil

1430
1450
Discovery of Novel Galabiosylceramide-Related Biomarkers of Fabry Disease by Semi-Targeted Metabolomics: the Complex Issue of Structural Isomer Interferences -
Michel Boutin
Université de Sherbrooke
Tackling Analytical Challenges in Sports Drug Testing by Mass Spectrometric Approaches -
Mario Thevis
German Sport University, Cologne, Germany
European Monitoring Center for Emerging Doping Agents (EuMoCEDA), Cologne/Bonn, Germany
Building Standards for Proteomics: A Targeted Mass Spectrometry Approach for Quantification of Cardiovascular Disease Biomarker in Human Blood -
Albert Sickmann
ISAS
Combining Mass Spectrometry Imaging & Microproteomics to Investigate Intratumor Heterogeneity -
Liam McDonnell
Fondazione Pisana per la Scienza ONLUS, Pisa, Italy
In-Depth Characterisation of Heavily Glycosylated Proteins and Their Potential as Colorectal Cancer Biomarkers -
Valeriia Kuzyk
Vrije Universiteit Amsterdam
EU LC-MS Verification -
Brian Keevil
Manchester University
1450
1510
Identifying the Metabolic “Achilles Heel” of Adult and Childhood Brain Cancers Using LC-MS-based Metabolite Profiling -
Dong-Hyun Kim
University of Nottingham
...
Keynote, continued
...
Validation of ApolipoProtein A-I Associated Lipoprotein Panel for the Prediction of Cholesterol Efflux Capacity -
Cory Bystrom
Cleveland HeartLab
...
Keynote, continued
...
Development of MS-based Prostate-Specific Antigen Test with In-Depth Glycosylation Analysis -
Guinevere S.M. Lageveen-Kammeijer
Center for Proteomics and Metabolomics, LUMC
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Switched with A Moran

...
Extended Session
...
1510
1530
Urinary Biomarkers of Dietary Intake - Quantification of Sugars for Epidemiology Studies Using LC-MS/MS -
Nicola Gray
University of Reading
...
Keynote, continued
...
Defining the Measurands for Antithrombin and its Proteoforms Using Mass Spectrometry Based Techniques -
Renee Ruhaak
Leiden University Medical Center
...
Keynote, continued
...
Characterisation of Prostate-Specific Antigen Isolated from Patients’ Urine -
Alan Moran
Leiden University Medical Centre
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Switched with G Lageveen-Kammeijer

...
Extended Session
...

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Wednesday
1530
1630
Poster Session 2
@ 1st Floor Exhibit Hall
678

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Wednesday
1530
1630
MEET-THE-EXPERTS : Poster Tours
@ 1st Floor Exhibit Hall
704

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1630

Distinguished Contribution Award Lecture


@ Mozart 1-5
Chair: Elizabeth Want & Brian Keevil

1630
1730

On the Award

Mass Spectrometry and the Elucidation of Steroid Metabolome
Cedric Shackleton
UCSF Benioff Children

Sterols and steroids were among the first biomolecule groups to be studied by mass-spectrometry. The early success of GC separation in 1960 by Sweeley and Horning and the combination of GC and MS by Ragner Ryhage in Stockholm with invention of a technique for separating the bulk of carrier gas from analytes prior to ionization were fundamental. This led in the mid-60s to the first commercial dedicated GC/MS instrument, the LKB 9000. Professor Jan Sj

Wednesday
1730
1930
Exhibitor Reception
@ 1st Floor Exhibit Hall

Buffet dinner, appetizers and drinks to be provided. Enjoy mingling with colleagues and Exhibitors. Take time to browse the posters.
680

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Wednesday
1830
1930
MEET-THE-EXPERTS : Office Hours Forum
@ 1st Floor Cafe

Have a question from the congress that you have been itching to ask? Or a problem from work that you want to get feedback on? Or feedback on the congress? Sign up at the registration desk for 15-min blocks to share time and ideas with domain experts in a relaxed setting.
681
1930
2030
Discussion Group
@ Papageno Hall
Wednesday
2030
Enjoy the City
@ Your Choice
683

Thursday

Thursday
745
900
Welcome Coffee
@ Entrance Foyer

Enjoy coffee, a pastry and a chat with colleagues before the day starts.
604
800
845

Corporate Workshop(s)


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Zivak Technologies
@ Paracelsus (Track 4)

Pre-Register

From Primary Sample Tubes to Results: World’s First Fully Automated Sample Preparation and Injection UHPLC System

Zivak Technologies is delivering solution to manual sample preparation related problems of chromatography analysis and enabling clinical laboratories to make the most of their MS/MS instruments by full automatization.

Fully automated Zivak analyzers allow you to get results from primary sample tubes without any user intervention. After the tubes are placed in the sample tray and the analysis is started on the computer, the robotic arms carry out all sample preparation steps, inject the prepared sample to MS/MS and chromatographic run starts. With Zivak analyzers, opening the sample tubes’ caps is never required.

Attend the workshop and listen a detailed review of World’s First Walk Away Sample Preparation and Injection UHPLC System Multitasker and Zivak’s Revolutionary VD-200, Fully Automated Vitamin D2-D3 UHPLC Analyzer from Prof. Dr. Ahmet Ceyhan Gören.

Thursday
800
900
SciCom Discussion Group
@ 1st Floor Cafe
856
Thursday
845
900
Intermission
@ Entrance Foyer
677
Thursday
900
1000
Exhibitor Feedback
@ 1st Floor Cafe

An opportunity for exhibiting vendors to provide feedback on what worked and what could use improvement.
691

Scientific Session 4


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Track 1
Mozart 1-3

Metabolomics – Clinical Studies I
Chair: Oleg Mayboroda

Track 2
Mozart 4-5

Quality Issues
Chair: Anne Bendt

Track 3
Papageno

Clinical Chemistry I
Chair: Éva Hunyadi-Gulyás

Track 4
Paracelsus

MSI for Clinical Applications
Chair: Raf Van de Plas

Track 5
Trakl

Microbiology Keynote
Chair: Jean Armengaud

Track 6
Doppler

Practical Training : Finding Your Analyte
Chair: Catarina Horro-Pita

900
920
Metabolomic Analysis of Changes in the Serum of Diabetic Patients Caused by Ulcers -
Arsenty Melnikov
Internatrional Tomography Center SB RAS
Quality Assurance and Quality Control in LC-MS-based Targeted Lipidomics of Human Blood Plasma -
Bo Burla
National University of Singapore
A Newly Developed LC-MS/MS Method for the Quantitation of Glucagon in Plasma Shows the Lack of Specificity of Former RIA Methods -
Jordi Farre-Segura
University of Liège, CHU de Liège
Next Generation Sequencing After MALDI MSI – A Feasibility Study -
Kristina Schwamborn
Institute of Pathology, TU Munich
Tandem MS-Proteotyping: Proteomics- and Genomics-based Characterization and Typing of Infectious Bacteria -
Edward Moore
University of Gothenburg
Where Did My Analyte Go? – coping with poor solubility and non-specific binding -
Catarina Horro Pita
LGC, Drug Development Solutions
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920
940
A Metabolite Level View of the Gut-Brain Axis in Dementia and Aging: Quantification of the Kynurenine/Tryptophan Pathway Using Mass Spectrometry -
Luke Whiley
Imperial College London
Stable Isotope-Dilution-LC-MS/MS-based Reference Methods – Suggestion for Description of Methods and Structure of Analytical Series -
Michael Vogeser
University Hospital, LMU Munich
Progress in Clinical Applications for the MALDI-Imaging Analysis of Thyroid Biopsies: Evaluation of Proteomic Stability in Preservative Solutions -
Isabella Piga
University of Milano-Bicocca
MALDI-MSI in the Search for Proteomic Indicators of Response to Therapy in Membranous Nephropathy -
Andrew Smith
University of Milano-Bicocca
...
Keynote, continued
...
...
Extended Session
...
940
1000
Estimation of the Post Mortem Interval via Metabolomic Approach -
Lyudmila Yanshole
International Tomography Center SB RAS
The Use of Ion Mobility Coupled with High Resolution Mass Spectrometry to Improve Separation and Identification of Lipid Biomarkers in Metabolic Diseases -
Julian Griffin
Department of Biochemistry, University of Cambridge, Cambridge, UK & University of Cagliari, Italy
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New Addition

Profiling Pathways of Estrogen Metabolism in Patients with Pulmonary Arterial Hypertension: Development of a Novel Assay for Estrogen Metabolites by LC-MS/MS -
Nina Denver
University of Glasgow
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New Addition

Bringing Mass Spectrometry to Pathologists for Diagnostics of Frozen Surgically Resected Tissue Specimen -
Tiffany Porta Siegel
M4I, Maastricht University
...
Keynote, continued
...
...
Extended Session
...

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Thursday
1000
1100
Poster Session 3
@ 1st Floor Exhibit Hall

Selected posters to be attended for 1 hour. Refer to program for posters attended during this period.
671

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Thursday
1000
1100
MEET-THE-EXPERTS : Poster Tours
@ 1st Floor Exhibit Hall
705
Thursday
1000
1100
Poster Contest Finalist Interviews
@ 1st Floor Exhibit Hall

Poster Contest Finalists to attend posters and meet with poster Judges. Judges may arrive in groups or individually.
708

Scientific Session 5


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Track 1
Mozart 1-3

Metabolomics – Clinical Studies II
Chair: Nicola Gray

Track 2
Mozart 4-5

Quantitation Issues
Chair: Christiane Auray-Blais

Track 3
Papageno

Clinical Proteomics II
Chair: Renee Ruhaak

Track 4
Paracelsus

Glycomics Keynote
Chair: Manfred Wuhrer

Track 5
Trakl

Technological Innovations for the Microbiology Laboratory
Chair: Ed Moore

Track 6
Doppler

Practical Training: Internal Standards
Chair: Russ Grant

1100
1120
Analytical Strategies to Study the Interaction Between Microbiota and Drug Metabolism Using Targeted and Untargeted LC-MS-based Metabolomics -
Marine Letertre
Imperial College London
Semi-Quantitative LC/ESI/MS Analysis Using Predictive Models of ESI Ionization Efficiencies -
Jaanus Liigand
University of Tartu
Paving the Way for MS-based Protein Tests that Target Unmet Clinical Needs -
Christa Cobbaert
Leiden University Medical Center
High-throughput Glycomics in Patient Stratification - What Did We Learn from the First 60,000 Analyses -
Gordon Lauc
University of Zagreb & Genos Glycoscience Research Laboratory, Zagreb, Croatia
Laser Assisted Rapid Evaporative Ionisation Mass Spectrometry: An Automated Platform for Direct-From-Culture Speciation and Clinical Sample Analysis -
Simon Cameron
Imperial College London
Everything You Wanted to Know about Internal Standards But Were Too Afraid to Ask -
Russ Grant
Labcorp
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1120
1140
Clinical Validation of an Automated Dried Blood Spot Sampling Device – Fluispotter® -
Khem Adhikari
Rigshospitalet
Quantification and Library Identification of 248 Compounds Commonly Detected in Systematic Toxicology Analysis Using an Automated Extraction Coupled to LC-MS/MS -
Robin Tiphaine
CHU of Limoges
Combination of Stable Labelled Antibodies and LC-MS to Accurately Quantify Therapeutic Monoclonal Antibodies in the Clinical Laboratory -
Ravindra Chaudhari
PROMISE Advanced Proteomics
...
Keynote, continued
...
In-situ Detection of the Botulinum Neurotoxin A by Functionalized MALDI Chips -
Petra Darebna
BioCeV
...
Extended Session
...
1140
1200
Use of Quantitative Metabolomics for Investigation of Age-Related Nuclear Cataracts -
Vadim Yanshole
International Tomography Center SB RAS
Clinical Mass Spectrometry: the Assessment of Acidosis Profiles and Acute Kidney Injury in Severe Malaria -
Natthida Sriboonvorakul
Mahidol University
Clinical Analytical Assay for AFP-L3 Using Multiple Reaction Monitoring-Mass Spectrometry for Diagnosing Hepatocellular Carcinoma -
Youngsoo Kim
Seoul National University College of Medicine
...
Keynote, continued
...
MALDI-TOF Mass Spectrometry: A Method for the Detection of Bacterial Contamination in Platelet Concentrates -
Yasmine Chetouane
IHU méditerranée infection
...
Extended Session
...
Thursday
1200
1330
Lunch
@ 1st Floor Exhibit Hall
693

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Thursday
1230
1330
Poster Session 4
@ 1st Floor Exhibit Hall

Selected posters to be attended for 1 hour. Refer to program for posters attended during this period.
706

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Thursday
1230
1330
MEET-THE-EXPERTS : Poster Tours
@ 1st Floor Exhibit Hall
707
Thursday
1330
EXHIBITS CLOSED
@ 1st Floor Exhibit Hall
695
1330
1415

Corporate Workshop(s)


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Tecan
@ Papageno (Track 3)

Meet our experts in MS sample prep who will present innovative workflows using positive pressure workstations and liquid handling platforms to prep samples for clinical proteomics and steroid analysis.

Semi-automated sample processing for clinical proteomics
Stefan Loroch, Leibniz Institute for Analytical Sciences (ISAS), Proteomics Facility

Powerful automated multi-steroid extraction via positive pressure and liquid handling platform for LC-MS analysis
Dr. Christian Scherling, Tecan Germany, MS Sample Prep Application Specialist

Thursday
1415
1430
Intermission
@ Entrance Foyer
696

Scientific Session 6


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Track 1
Mozart 1-3

Metabolomics Keynote
Chair: Russ Grant

Track 2
Mozart 4-5

Monitoring Metabolites in Inborn Errors of Metabolism
Chair: Zdenek Spacil

Track 3
Papageno

Oncology
Chair: Yuri van der Burgt

Track 4
Paracelsus

Tissue Imaging Keynote
Chair: Kristina Schwamborn

Track 5
Trakl

Biomarkers in Infectious Disease
Chair: Stefan Zimmerman

Track 6
Doppler

Practical Training : Getting to Lower Limits of Quantitation
Chair: Brian Rappold

1430
1450
How Tandem Mass Spectrometry Revolutionized Newborn Screening -
David Millington
Duke University School of Medicine
Development of a 2D-UPLC-MS/MS Assay for Therapeutic Monitoring for Patients with APRT Deficiency -
Unnur Arna Thorsteinsdottir
University of Iceland
Proteomic Analysis of MGL Binding Protein in Human Cancer Cells -
Martina Pirro
Leiden University Medical Center LUMC
Advances in Computational Methods for Tissue Imaging by Mass Spectrometry -
Raf Van de Plas
Delft University of Technology
Omics Approaches Towards Discovery of Biomarkers for Early Diagnosis of Tuberculosis -
Ana Varela Coelho
ITQB NOVA
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Switched with M Kostrzewa

1/3 Getting Lower Limits of Quantification in LC-MS/MS assays : Prologue -
Brian Rappold
LabCorp
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1450
1510
...
Keynote, continued
...
The Role of Chromatography in the Separation of Glucosylceramide Isoforms from Their Isobaric Galactosylceramide Counterparts in the Era of Precision Medicine -
Christiane Auray-Blais
Universite de Sherbrooke
Exosome-like Vesicles of Uterine Aspirates Permit the Identification of Diagnostic and Stratification Biomarkers of Endometrial Cancer -
Eva Coll de la Rubia
Vall Hebron Research Institute
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Replacing Eva Colas. Same Lab & Abstract.

...
Keynote, continued
...
Sepsis by Enterobacteria: A MALDI Affair -
Markus Kostrzewa
Bruker Daltonik GmbH
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Switched with Ana Varela Coelho

2/3 Getting Lower Limits of Quantification in LC-MS/MS assays : Phases and Stages -
Brian Rappold
LabCorp
1510
1530
...
Keynote, continued
...
First Experience with a Second-Tier LC-MS/MS Assay for Newborn Screening of Propionic Acidemia, Methylmalonic Acidemias and Combined Remethylation Disorders -
Glynis Klinke
University of Heidelberg
In Depth Proteomic Analysis of Prostate Cancer Biopsies -
Gabor Toth
Hungarian Academy of Sciences
...
Keynote, continued
...
Identification and Validation of Two Peptide Markers for the Recognition of Clostridium difficile MLST-1 and MLST-11 by MALDI-MS -
Simone Nicolardi
Leiden University Medical Center
3/3 Getting Lower Limits of Quantification in LC-MS/MS assays : The Words Don’t Fit the Picture -
Brian Rappold
LabCorp
Thursday
1530
1545
Petite Reception
@ Mozart 4-5

Passed beer, wine and other beverages while we prepare for the poster awards.
697
Thursday
1545
1600
Poster Award Presentation
@ Mozart 4-5
698

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1600

Closing Plenary Lecture


@ Mozart 4-5
Chair: Guinevere Lageveen-Kammeijer

1600
1645
Mass Spectrometric Analysis of Glycomic Signatures of Cancer and Autoimmune Diseases: Towards Clinical Application
Manfred Wuhrer
LUMC

Many major human diseases including various types of cancer and autoimmune diseases are associated with protein glycosylation changes. These changes are often instrumental in disease development and progression. In cancer, glycosylation changes of both tumor tissues and tumor-derived circulating antigens have been described. However, diagnostic test often merely rely on the measurement of antigen concentrations and fail to register glycosylation changes, largely due to the lack of suitable technology and workflows. In this presentation, examples will be given of recently developed mass spectrometric workflows to determine disease-associated glycosylation changes from tumor tissues and in the circulation, but also from other body fluids including urine and saliva. Promising markers will be presented including immunoglobulin G glycosylation, cancer glycoprotein antigens, as well as tissue glycosylation signatures revealed by mass spectrometry imaging. Steps towards the clinical translation of these markers will be discussed.

Thursday
1645
1800
Closing Reception & Discussion
@ Entrance Foyer

Wine & Cheese, Beer & Nuts ... and maybe Kaiserschmarrn. Winning posters to be on display.
700
Thursday
1800
2200
After Congress Meet-Up
@ Your Choice

Meet up at the Augustiner Braustubl (Historic Abby turned beer garden with food stalls) for an unofficial and unsponsored post-con gathering.
699