MSACL 2020 US Program

MSACL 2020 US : Conference Program

Palm Springs, CA • March 29 - April 3, 2020

2020 US Sponsors

Abstract Clinical Use Status Key
= Emerging. More than 5 years before clinical availability. (26.62%)
= Expected to be clinically available in 1 to 4 years. (38.91%)
= Clinically available now. (34.47%)


Sunday

800
1800
Badge Pickup
@ RegDesk @ Ballroom Foyer
800
1200

Short Course

COURSE START
Ending at 6PM on Sunday and Monday
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD, Deborah French, PhD & Tim Collier, PhD
Level: Beginner - Intermediate
@ Room 5
Group: B
1200
1300
Lunch
@ The Palmetto Restaurant
1300
1800

Short Course

Continued from Sunday AM
Ending at 6PM on Sunday and Monday
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD, Deborah French, PhD & Tim Collier, PhD
Level: Beginner - Intermediate
@ Room 5
Group: B
1400
1800

Short Course

COURSE START
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner
@ Room 6
Group: B
COURSE START
Cancelled
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Beginner-Intermediate
@ Room 1
Group: A
COURSE START
Cancelled
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Room 3
Group: A
COURSE START
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Room 2
Group: A
COURSE START
Cancelled
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ TBD
Group: A
COURSE START
Cancelled
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
Level: Beginner - Intermediate
@ Room 4a
Group: C
1800
2000
Poolside Dinner
@ Chiki Pool
2000
2100
Hospitality Lounge
@ Chiki Pool
2000
2100
Hospitality Lounge (Rain)
@ The Landing

Monday

700
1800
Badge Pickup
@ RegDesk @ Ballroom Foyer
700
830
Breakfast
@ Gypsy Rose, Palmetto & Cantala
730
1130

Short Course

Continued from Sunday
Cancelled
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Beginner-Intermediate
@ Room 1
Group: A
Continued from Sunday
Cancelled
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Room 3
Group: A
Continued from Sunday
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Room 2
Group: A
Continued from Sunday
Cancelled
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ TBD
Group: A
800
1200

Short Course

Continued from Sunday
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner
@ Room 6
Group: B
Continued from Sunday
Ending at 6PM on Sunday and Monday
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD, Deborah French, PhD & Tim Collier, PhD
Level: Beginner - Intermediate
@ Room 5
Group: B
COURSE START
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mari L. DeMarco, PhD DABCC FACB FCACB & Teesha Luehr
Level: Beginner - Intermediate
@ The Boardroom
Group: B
830
1230

Short Course

COURSE START
CANCELLED
Data Science 203
Machine Learning : A Gentle Introduction
Stephen Master, MD, PhD & Randy Julian, PhD
Level: Intermediate
@ Room 8
Group: C
COURSE START
Imaging 202
"iMSACL": Clinical Studies using Imaging Mass Spectrometry
Michelle Reyzer, PhD, and Christina Ferreira, PhD, and Sankha (Bobby) Basu, MD, PhD
Level: Beginner-Intermediate
@ Room 4b
Group: C
COURSE START
Lipidomics 101
Mass Spectrometry-based Lipidomics and Clinical Applications
Anne K Bendt, PhD, Amaury Cazenave-Gassiot, PhD & Michael Chen, MD MSc
Level: Beginner - Intermediate
@ Room 7
Group: C
Continued from Sunday
Cancelled
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
Level: Beginner - Intermediate
@ Room 4a
Group: C
COURSE START
Toxicology 201
Advanced Forensic Toxicology
Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Level: Intermediate - Advanced
@ Room 8
Group: C
1130
1330
Short Course Lunch
@ Cantala Restaurant
1230
1630

Short Course

Continued from Monday AM
Cancelled
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Beginner-Intermediate
@ Room 1
Group: A
Continued from Monday AM
Cancelled
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Room 3
Group: A
Continued from Monday AM
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Room 2
Group: A
Continued from Monday AM
Cancelled
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ TBD
Group: A
1300
1800

Short Course

Continued from Monday AM
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner
@ Room 6
Group: B
Continued from Monday AM
Ending at 6PM on Sunday and Monday
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD, Deborah French, PhD & Tim Collier, PhD
Level: Beginner - Intermediate
@ Room 5
Group: B
Continued from Monday AM
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mari L. DeMarco, PhD DABCC FACB FCACB & Teesha Luehr
Level: Beginner - Intermediate
@ The Boardroom
Group: B
1330
1730

Short Course

Continued from Monday AM
CANCELLED
Data Science 203
Machine Learning : A Gentle Introduction
Stephen Master, MD, PhD & Randy Julian, PhD
Level: Intermediate
@ Room 8
Group: C
Continued from Monday AM
Imaging 202
"iMSACL": Clinical Studies using Imaging Mass Spectrometry
Michelle Reyzer, PhD, and Christina Ferreira, PhD, and Sankha (Bobby) Basu, MD, PhD
Level: Beginner-Intermediate
@ Room 4b
Group: C
Continued from Monday AM
Lipidomics 101
Mass Spectrometry-based Lipidomics and Clinical Applications
Anne K Bendt, PhD, Amaury Cazenave-Gassiot, PhD & Michael Chen, MD MSc
Level: Beginner - Intermediate
@ Room 7
Group: C
Continued from Monday AM
Cancelled
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
Level: Beginner - Intermediate
@ Room 4a
Group: C
Continued from Monday AM
Toxicology 201
Advanced Forensic Toxicology
Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Level: Intermediate - Advanced
@ Room 8
Group: C
1630
1900
Hospitality Lounge
@ Gypsy Rose

Meet up at the Gypsy Rose for a drink before heading to the dinner location of your choice.
1730
1930
Dinner on Own
@ YourChoice

There will be food for purchase available at The Riviera Dining locations, or visit something offsite.
Some places to try in walking distance:
1. Draughtsman, 3:00pm to 10pm, Food, large selection of Craft Beers, outdoor games. Settle in.
2. Sandfish, Excellent Sushi and whiskey pairings. 4:30 to 10pm. Reservation recommended.
3. Billy Reeds, American comfort food, good for large groups. 8am-9pm.
4. JJ's Latin Oasis, Mexican food, Chris Herold's favorite stop in Palm Springs since 1995.

A wee bit further:
1. Copleys, Fine Dining (reservation)
2. Workshop, Contemporary (reservation)
3. Purple Palm Poolside Mediterranean (reservation)
4. Roly China Fusion 13 minute walk
5. For an adventure, dine at the top of Mt. San Jacinto after a ride on the Palm Springs Aerial Tram

Have an ice cream after dinner at ICE CREAM SHOPPE open noon to 11pm.
1830
2000
Private Faculty Dinner
@ Chiki Pool

By Invitation - Faculty including Scientific Committee members and Course Instructors as well as invited speakers and CMS Journal Editors may convene to touch base prior to the opening of the conference.

Tuesday

700
2000
Badge Pickup
@ RegDesk @ Ballroom Foyer
700
830
Breakfast
@ Gypsy Rose, Palmetto & Cantala

For short course attendees with class Tuesday AM.
730
1130

Short Course

Continued from Monday PM
Cancelled
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Beginner-Intermediate
@ Room 1
Group: A
Continued from Monday PM
Cancelled
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Room 3
Group: A
Continued from Monday PM
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Room 2
Group: A
Continued from Monday PM
Cancelled
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ TBD
Group: A
800
1200

Short Course

Continued from Monday PM
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner
@ Room 6
Group: B
Continued from Monday PM
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mari L. DeMarco, PhD DABCC FACB FCACB & Teesha Luehr
Level: Beginner - Intermediate
@ The Boardroom
Group: B
0840
1150
Get the Basics
@ Rooms 4-6
Room 4 Chairs: Jen Colby and Jackie Hubbard
Room 5 Chairs: David Herold and Peggi Angel

An overview of the fundamentals that are driving research and development.
This series of Primers on fields within Clinical Mass Spec is free to attend for ALL attendees of the MSACL Congress or Short Courses, including Partial Pass holders.

Takeaways for each session include:

  1. Be able to define what the field is, why it matters, and why you personally should care. What is the clinical relevance?
  2. Understand what role mass spectrometry plays in this field. Where does mass spec fit in to the big picture of the field?
  3. Define any terminology that is specific to this field.
  4. Describe how it works, what are the methods and workflows used when studying this field and/or how is it implemented in clinical labs.
  5. Identify any challenges to implementation/adoption, where do the opportunities lie?

08:40 - 09:20
  • Room 4: Metabolomics with Adam Rosebrock
  • Room 5: Proteomics with Tim Collier
09:30 - 10:10
  • Room 4: Lipidomics with Anne Bendt
  • Room 5: Glycomics with Richard Drake
10:20 - 11:00
  • Room 4: Toxicology with Paul Jannetto
  • Room 5: Imaging Mass Spectrometry with Ron Heeren
11:10 - 11:50
  • Room 4: Sample Preparation with Mark Marzinke
  • Room 5: Data Science with Alfonso Limon
11:50
1400
Lunch on your own
@ YourChoice

Join a Workshop, where a Sandwich Lunch will be served. Alternatively, enjoy lunch onsite at one of the Riviera dining spots, or offsite with fellow attendees.
If you would like to head offsite:
1. Wexler's Deli
2. Billy Reeds, American comfort food, good for large groups. 8am-9pm.
3. JJ's Latin Oasis, Mexican food
4. Roly China Fusion, 13 minute walk
5. Trio One mile away, 20 minute walk.
6. Cheekys Popular for Brunch and Bacon Flights. One mile away, 20 minute walk.
7. Mid Mod Cafe, for the health conscious, 3 minute drive
8. Tac/Quila, elevated Mexican Cuisine, 3 minute drive
1100
1600
Place Wed Posters
@ Exhibit Hall
1215
1345

Lunch Vendor Workshop(s)

Indigo BioAutomation
@ Rm 1-3

Pre-Register

Let the Data Speak. Data Driven Decision Making for Mass Spectrometry.
Moderator: Randall Julian, Ph.D., CEO and Founder, Indigo BioAutomation

Speakers:
Romeo Solano, Ph.D., F-ABFT, Senior Scientific Director, Quest Diagnostics

Why do staff migrate to certain instruments when they need to report results within a defined turn-around time? Is everyone following your SOPs? Big data analysis can provide insights into operational transformation of processes in every laboratory, show how instruments and staff members are performing, and guide root cause analysis to drive actionable solutions. Using Tableau to analyze ASCENT flags and decisions with respect to Calibrators, QCs, and Unknowns, this presentation will demonstrate how effective changes to LC-MS/MS procedures can benefit process and reduce data review time.

Will Slade, Ph.D., Systems Scientist, Indigo BioAutomation

Data review in high-throughput settings traditionally involves monotonous evaluation of every chromatogram, making review into an art as much as a science. This risks a confidence coming from manually inspecting a plot instead of trusting established metrics. Using ASCENT and R statistical programming language, this presentation will describe Indigo’s journey into the largely unexplored wealth of big data already being collected, what we have learned, and where we want to go.

1400
1415
Welcome Orientation
@ Rooms 4-6
1415

Opening Plenary Lecture


@ Rooms 4-6
Chair: Livia Eberlin

1415
1500
Comprehensive Mapping of Cell Lineage and Function in Human Tissue Using MIBI-TOF
Michael Angelo
Stanford Bio-X

1500
1515
Intermission
@ Ballroom Foyer
1515
1600

State of the Science Address
@ Rooms 4-6

Chairs: Cory Bystrom and Dan Holmes
Speakers: Patrick Mathias, Adam Rosebrock, Chris Cox, Grace van der Gugten, Chris Shuford, Peggi Angel, Jennifer Colby
Presented by MSACL US Scientific Committee Topic Leads, this address will provide an overview of the applications and technologies currently being used in Clinical Labs, and a clear view of the development pipeline. It will highlight applications expected to be available in the near-future, as well as emerging applications, and key contributors. Relevant talks, posters, and people present at the congress will be identified, enabling you to optimize your learning path and more effectively target potential network connections. Whether you are new to Clinical Mass Spectrometry, or a seasoned veteran, the State of the Science address should be on your agenda.
1600
1615
Intermission
@ Ballroom Foyer
1615
1645
Poster Lightning Talks
@ Rooms 4-6

Chair: Cory Bystrom
Poster Contest semi-finalists will highlight their work in 90 seconds, and one slide.
1645
1715
Vendor Lightning Talks
@ Rooms 4-6

Chair: Chris Herold
An opportunity to discover vendors and learn what's new. Up to 20 vendors will be highlighted, each given 90 seconds and one slide.
1715
2000
Exhibits Opening
@ Exhibit Hall
1800
1900
Meet-a-Mentor: Booth Tours
@ Exhibit Hall

Join a mentor as they tour the booths and learn more about technologies available, what mentors are looking for during the exchange, and how they interact with the booth vendors. Increase your network and your knowledge of products and services available.
1900
2000
Troubleshooting Forum: Poster Rounds
@ Exhibit Hall

Join the troubleshooting poster discussion to learn something new or add your two cents.
19:00 @ poster #93a
“Continental Drift” and Varying Vitamin D Results Dependent Upon Methanol Reagent Lot
Joshua Akin
UC San Diego Health
19:15 @ poster #94a
Recovery of Insulin-like Growth Factor 1 (IGF-1) Signature Peptides
Amol Bajaj
ARUP Laboratories
19:30 @ poster #95a
Steroid Assay Development Nightmare – Analytes in Every Sample and Everywhere
Katerina Sadilkova
Seattle Children’s Hospital
19:45 @ poster #96a
Distinguishing Contamination from Carryover When Using LC-MS/MS to Detect Drugs of Abuse
Kristine van Natta
Thermo Fisher Scientific
2000
2100
Discussion Group: CDC Clinical Standardization Programs Forum
@ Room 1
Chair: Hubert Vesper
Overview of ongoing and new CDC’s Standardization Programs
2000
2100
Discussion Group: From the Operating Room to the Mass Spec: Handling and Storage of Human Tissue Samples
@ Room 2
Chair: Chris Anderton
Hosted by IMSS.

We will discuss patient-specific effects, sample collection, transport and handling of samples, and tissue preparation when acquiring and interpreting mass spectrometry data. We will especially focus on how these steps effect imaging mass spectrometry data, but many of these topics translate universally across mass spectrometry approaches in general.

This will be an open forum with predefined topics to guide the conversation.
2000
2100
Discussion Group: R/Medicine - Integrating R into Clinical Practice
@ Room 3
Hosted by R/Medicine and moderated by Stephan Kadauke.
Speakers: Stephan Kadauke, Michael Kane, Joseph Rickert

R is a free programming language and a powerful statistical computing environment that is wildly popular among statisticians and data scientists. R is ideally suited for building customized applications for clinical operations, however many (real and perceived) hurdles exist in operationalizing R in healthcare in general, and in the labs in particular. The speakers are the organizers of R/Medicine, an annual conference that aims to promote the R ecosystem in clinical research and practice. This will be a highly interactive session aimed to share insights and experiences both of the discussants and participants, with the aim of facilitating the integration of the R ecosystem into clinical laboratory practice.
Learning objectives:
After completing this activity, participants will be able to:
1. Cite resources available for R and data science education tailored for clinicians and laboratorians.
2. Describe organizational, regulatory, and cultural challenges to integrating the R ecosystem into clinical practice.
3. Discuss best practices for building customized clinical laboratory-developed applications (“lab apps”) in R/Shiny.
Speakers:
Stephan Kadauke, Assistant Professor of Pathology and Laboratory Medicine at the Children’s Hospital of Philadelphia
Michael Kane, Assistant Professor of Biostatistics at Yale University, past Chair of R/Medicine
Joseph Rickert, RStudio PBC, and the R Consortium
2000
2100
Discussion Group: Clinical Cases – Where Mass Spec made a Difference in Patient Care
@ Room 4
Hosted by AACC and moderated by Carmen Wiley.
Speakers: Nicole Tolan, Dennis Dietzen, and Stephen Master
Mass spectrometry continues to play a prominent role in the field of laboratory medicine. In this session, each speaker will highlight a clinical case where Mass Spec Analysis has made all the difference in a patient's care and outcome.
Learning objectives:
After completing this activity, the learner will be able to:
1. site examples where mass spectrometry made a difference in patient care
2. describe how mass spectrometry could play an important in their institutions
3. summarize the role of mass spectrometry played in each of these case studies
Speakers:
Nicole Tolan, Assistant Professor of Pathology at Harvard Medical School
Dennis Dietzen, Professor, Pathology & Immunology at Washington University School of Medicine
Stephen Master, Associate Professor of Pathology and Laboratory Medicine at the Children's Hospital of Philadelphia
2000
2100
Discussion Group: Developing Mass Spectrometric Assays for Obesity and Diabetes Clinical Research
@ Room 6
Moderator: Salvatore Sechi, NIDDK/NIH
Presenters/Discussants include: Jenny Van Eyk (Cedars Sinai); Wei-Jun Qian (PNNL); Andy Hoofnagle (University of Washington)

The NIDDK recently started a program for developing mass spectrometric assays for proteins and peptides of primary interest to the obesity and diabetes research community. These assays should be highly reproducible, easily transferable to other laboratories, and validated in human plasma or serum. Examples, of proteins and peptides that are of potential interest include insulin, glucagon, c-pepitide, glycated CD59, Adiponectin, Leptin, Resistin, Neuropeptide Y, Alpha-melanocyte-stimulating hormone, Peptide YY, Glucagon-like peptide 1, Ghrelin, Adrenocorticotropin, Corticotropin-releasing hormone, Gastrin, Cholecystokinin, Secretin, Vasoactive intestinal peptide, gastric-inhibitory peptide, gastrin-releasing peptide, motilin, pancreatic polypeptide, RBP4, myostatin, FGF21).

We will discuss how we might proceed in developing, validating, and implementing MS assays of interested to the Obesity and Diabetes community.
2000
2200
Hospitality Lounge
@ Soleil Pool

Wednesday

700
1800
Badge Pickup
@ RegDesk @ Ballroom Foyer
800
845

Corporate Workshop(s)

Biotage
@ Rm1

Pre-Register

One Size Does Not Fit All: Considerations for Minimizing Matrix Effect and Maximizing Recovery for Clinical Panels Large and Small

Dr. Lee Williams.
Biotage GB Limited, Distribution Way, Dyffryn Business Park, Hengoed, CF82 7TS, UK.

LC-MS/MS system sensitivity has moved at an alarming rate over the last 10 years. The original perception that due to increased instrument sensitivity sample preparation will decrease has been dispelled. This sensitivity can come at a price, in that levels of contamination not previously detected with less sensitive instruments now have larger impact on analysis. Combining this with the advent of UHPLC, the necessity for high purity components and samples is even more apparent. Given this information the choice of sample preparation technique varies between labs and is generally dependent on several factors: operator familiarity; technique complexity; price per sample; instrument sensitivity; assay sensitivity and limits of quantitation; sample volume; throughput; analyte functionality and target matrix.

Typical clinical matrices include whole blood, plasma, serum, urine and oral fluid with specific emphasis on a variety of endogenous components. Matrix complexity will be illustrated and related to a variety of clinical assays, demonstrating potential impact on method robustness. Examples will detail challenges involved for large panel analysis.

Thermo Fisher Scientific
@ Rm2

LC-MS/MS Developments for the Routine Clinical Lab

Overview:
Accurate analyte measurements are important for assessment and management of patients. LC-MS/MS measurement procedures are more selective than immunoassay measurement procedures. However, increased labor for preanalytical processing, method validation for LDTs, in conjunction with lack of complete automation of quality assurance monitoring, have presented barriers to LC-MS/MS implementation. This workshop will provide the opportunity to discuss the quality assurance needs of the routine clinical laboratory, the challenges associated with managing laboratory developed tests (LDTs) and the perspectives on future LC-MS/MS developments.

*Product is not 510(k) cleared and is not yet available for sale in the U.S.

MilliporeSigma
@ Rm3

How is the emergence of hemp affecting the Clinical space?

Synthesis, certification and clinical evaluation of 7-Hydroxy and 7-Carboxy Cannabidiol Certified Reference Materials in whole blood samples by LC-MS/MS

Speakers: Heather Lima - Principal Scientist, Synthesis, Cerilliant Corporation, Ray Suhandynata – Clinical Chemistry Fellow, University of California-San Diego Center for Advanced Laboratory Medicine

Cannabidiol (CBD), a non-psychoactive constituent of hemp, has been increasingly promoted and studied for pharmacological uses as regulations regarding cannabis and hemp evolve rapidly at a state and federal level. In response to these recent regulations, novel Cannabinoid Certified Reference Materials (CRMs) and testing methods have been developed for the main human metabolites of CBD, 7-hydroxy cannabidiol (7-OH CBD) and 7-carboxy cannabidiol (7-COOH CBD). We will touch on recent legal changes for cannabinoid testing in a Clinical setting, as well as discuss the synthesis, certification, and evaluation of these cannabinoid CRMs in patient whole blood samples at the Center for Advanced Laboratory Medicine at UCSD.

SCIEX
@ Rm4

Pre-Register

Acoustic Ejection Mass Spectrometry (AEMS) for High-throughput Analysis
Chang Liu, Associate Staff Research Scientist, SCIEX

Learn about the newly designed, novel mass spectrometry (MS) platform with integrated acoustic droplet ejection (ADE) technology. The open-port interface (OPI) paired with electrospray ionization (ESI) MS utilizes nanoliter droplets in a touchless manner to deliver high speed, precision and accuracy. The sample dilution within the OPI in concert with the capabilities of modern ESI-MS minimizes assay development. The combination, almost eliminates the laborious sample preparation required for both clean and dirty matrices such as plasma and urine, and results in no observed carry-over. See how second-per-sample analysis speed can be routinely achieved with high sensitivity, high reproducibility, wide linear dynamic range, and broad compound coverage.

Agilent Technologies
@ Rm5

Pre-Register

Reducing the Steps from Sample Submission to Reporting

Implementing a Walk-Up sample submission process with automated data processing reduces the training, labor and time required to analyze clinical diagnostic samples.
 
For In Vitro Diagnostic Use

Indigo BioAutomation
@ Rm6

Pre-Register

Elevating your lab further.
Speakers: Jim Edwards and Adam Zabell, Ph.D.

We’ve been helping labs elevate their business performance and technical efficiency with a simple vision: better science to generate better results. Those results are based on great data, effective models and comprehensive quality awareness, but moving beyond the batch requires a better toolbox so people at every level of the laboratory can integrate their effort into enhancing the entire business. Our latest innovations have come together in ASCENT Series 4, streamlining batch review and adding the features you need to identify not just if but when and why your assay is meeting your expectations. Please join us for an introduction to a better way to work, whether that means migrating from your incumbent software or upgrading your current ASCENT platform. We’ve brought together the best discoveries based on what we’ve learned working side-by-side with laboratories like yours, all in service of the tools that make for better science.

845
900
Intermission
@ Ballroom Foyer

Scientific Session 1

Track 1
Room 1

Tox/TDM/Endocrine: Order of Operations: Workflow Optimization and Quality Improvements for Mass Spectrometry
Chair: Stephen Roper
2nd Chair: Scott Borden

Track 2
Room 2

Standardization and Normalization of Metabolomic and Lipidomic Workflows
Chair: Melanie Odenkirk

Track 3
Room 3

Data Science Keynote: Olga Vitek
Chair: Randall Julian

Track 4
Room 4

Imaging MS: In the Clinic
Chair: Peggi Angel
2nd Chair: Anna Krieger

Track 5
Room 5

Proteomics: Innovating New Biomarkers
Chair: Stephen Pennington

Track 6
Room 6

Practical Training
Chair: Hema Ketha
2nd Chair: Deborah French

900
920
A Suggested Standard for Validation of LC-MS/MS-based Analytical Series (batches) in Diagnostic Laboratories
Judith Stone
Univ of California, San Francisco Health
Quality Control Strategies for Untargeted Clinical Metabolomics
Lisa Ford
Metabolon, Inc
Components of Reproducible Quantitative Mass Spectrometry-based Proteomics: A Statistician’s Perspective
Olga Vitek
Northeastern University
A Rapid MALDI Mass Spectrometry Imaging Method to Complement the Frozen Section Procedure in Surgical Pathology
Sankha Basu
Brigham and Women’s Hospital, Harvard Medical School
Quantification of Fully and Partially Acetylated HMGB1 Protein in the Serum and Pleural Effusion of Mesothelioma and Non-mesothelioma Individuals
Weston Spivia
Cedars Sinai Medical Center
Mastering Mass Spectrometry in the Clinic – Cases and Chronicles
Hema Ketha, Jennifer Colby
920
940
HarmCheck: Paper Spray Mass Spectrometry (PS-MS) for Harm Reduction Drug Checking and its Utility for the Opioid Overdose Crisis
Chris Gill
Vancouver Island University & University of Victoria
Development of Clinical Lipidomics Accompanied with Standardization and Harmonization by the International Lipidomics Society (ILS)
Kim Ekroos
Lipidomics Consulting Ltd
...
Keynote, continued
...
Intraoperative Use of the MasSpec Pen Technology for Ex Vivo and In Vivo Analysis of Human Breast Tissues
Kyana Garza
The University of Texas at Austin
Development of a Multiplexed Urine Protein Panel for Prognosis of Chronic Kidney Disease
Angela Mc Ardle
Cedars Sinai Medical Centre
...
Extended Session
...
940
1000
How Ion Trap Mass Spectrometry Changed Our Toxicology Workflow
Nicholas Laude
Genotox Laboratories
Storage Conditions of Human Kidney Biopsies Affects Spatial Metabolomics Analysis Reproducibility
Jessica Lukowski
Pacific Northwest National Laboratory
...
Keynote, continued
...
Rapid Evaporative Ionization Mass Spectrometry – Towards real-time molecular profiling of tissue during skin cancer surgery
Natasja Janssen
Queen’s University
The 3D Proteome and the Potential for Conformational Biomarkers
John Yates
Scripps Research
...
Extended Session
...
1000
1100
Exhibits & Poster Session
@ Exhibit Hall

Even 'a' poster positions to be attended.

Scientific Session 2

Track 1
Room 1

Not Your Average Cannabinoids
Chair: Jen Colby
2nd Chair: Chris Koch

Track 2
Room 2

Beyond Metabolomics -or- Lipidomics: Mutli-omic Analysis of Patient Samples
Chair: Elizabeth Want

Track 3
Room 3

Data Flow
Chair: Will Slade

Track 4
Room 4

Imaging MS Keynote: Lingjun Li
Chair: Richard Drake

Track 5
Room 5

Proteomics: Clinical Biomarker Assays - Revamped
Chair: Chris Shuford

Track 6
Room 6

Practical Training
Chair: Elizabeth Frank
2nd Chair: Jacqueline Hubbard (pending)

1100
1120
Clearing a Haze of Confusion; Differentiating CBD Use from Marijuana Use in Urine Drug Testing
Gregory Janis
Medtox / Labcorp
Full-scan Metabolomic Profiling of Glioblastoma Stem Cells Discovers Metabolic Pathway Variation Associated with Clinical Outcomes
Amy Caudy
Stony Brook University
Applied “R” for Error Reduction: Hands-free Seamless LC-MS/MS Batch Data Flow
Hema Ketha
Labcorp
Mapping the Chemical Space of Biological Systems via MALDI Mass Spectrometric Imaging and <i>in situ</i> Molecular Analysis
Lingjun Li
University of Wisconsin - Madison - Madison, WI
Clinical Validation of an LC-MS/MS Assay for Measurement of Serum C-peptide
Benjamin Owusu
University of Washington
Free Climb or Free Solo: Guidance and Accounts of Establishing Reference Intervals for LDT Mass Spectrometry Assays
Elizabeth Frank, Kelly Doyle
1120
1140
Correlation of Vitamin K1 Treatments with Plasma Concentrations of Anticoagulant Rodenticides following Inhalation of Contaminated Synthetic Cannabinoids
Richard van Breemen
Oregon State University
A Multi-omic Assessment of Unique Alzheimer’s Disease Mechanisms Reflective of ApoE Genotype Differences
Melanie Odenkirk
North Carolina State University
Speaking to Your Mass Spectrometer: A Toolkit to Visualize Mass Spectrometer Data
Abed Pablo
University of Washington
...
Keynote, continued
...
Dilute, Digest and Detect: Quantification of C-reactive Protein in Human Plasma by LC-MS/MS
Meng Wang
University of British Columbia
...
Extended Session
...
1140
1200
Development of an LC-MS/MS Assay for the Analysis of Endogenous Cannabinoid Plasma Concentrations
Raymond Suhandynata
University of California San Diego
Moving Forward Towards Diagnostic for Predisposition to Food Allergy and Atopic Dermatitis Through LC-MS/MS Analysis of Stratum Corneum on Skin Tape Strips
Evgeny Berdyshev
National Jewish Health
Data Analytics Hose to Data Analytics Pipeline
Wesley Hampton
Nationwide Children’s Hospital
...
Keynote, continued
...
Breaking with convention: Calibration strategies for proteolysis-aided workflows
Mari DeMarco
University of British Columbia
...
Extended Session
...
1200
1400
Lunch On Own
@ YourChoice

Maximize learning opportunities by popping into a Workshop where a sandwich lunch will be served, or enjoy a meal at one of The Riviera dining options, or take a walk to a nearby option such as Wexler's or Rick's Desert Grill.
1230
1330

Corporate Workshop(s)

Shimadzu
@ Rm1

Developing a Comprehensive Mass Spectrometry Program at an Academic Medicine Center

Chris Crutchfield, PhD, HCLD(ABB), DABCC, Assistant Professor, University of Cincinnati College of Medicine, Department of Pathology & Laboratory Medicine

The advent of early career clinical scientists trained in mass spectrometry has arrived. A practical challenge for those clinical scientists entering their first jobs at academic medical centers will be how to navigate the assembly or the expansion of clinical mass spectrometry within the laboratory, as well as how to balance the needs of meeting expectations for both routine clinical work and academic research.

This workshop will include a series of vignettes developed in the context of expanding mass spectrometry at an academic medical center:

• Partnering with a business director to develop a business rationale to justify acquisition of two mass spectrometers to expand immunosuppressant monitoring

• Joining a research team as a co-PI, receiving a perfect score on an NIH U01 grant, “IND-Enabling Pre-Clinical Studies to Accelerate the Clinical Development of a Humanized Anti-Cocaine Monoclonal Antibody,” and including a mass spectrometer in the approved budget

• Pearls of Wisdom for training medical technologists and research technicians clinical mass spectrometry assay preparation and method development

Thermo Fisher Scientific
@ Rm2

Paper Spray Mass Spectrometry (PS-MS) for Direct, Quantitative Measurements

Professor Chris G. Gill, Ph.D., P. Chem., Co-Director, Applied Environmental Research Laboratories, Vancouver Island University, Nanaimo, BC, Canada

Paper spray ionization for mass spectrometry is a relatively new, simple, and inexpensive strategy for ambient ionization. Analytes are ionized for mass spectrometry directly from small volumes of complex samples deposited on pointed paper strips, in a mechanism akin to electrospray. It is particularly useful where rapid, direct quantitative chemical measurements are needed. We present PS-MS in select applications: direct drug analysis in urine, and onsite illicit drug checks for harm reduction in the opioid overdose crisis.

Lipidomics Analysis of Barth Syndrome Patients

Seul Kee Byeon1, Devin Oglesbee1, and Akhilesh Pandey1,2, 1Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester MN 55905, 2Center for Individualized Medicine, Mayo Clinic, Rochester MN

Barth syndrome (BTHS) is a rare X-linked recessive disorder that presents with infantile cardio- and skeletal myopathy, neutropenia, and growth delays. We hypothesized rare subtypes of various classes of lipids may contribute to BTHS. We characterized various classes of lipids and discovered lipid profiles associated with BTHS. Our studies show that unique lipid signatures can differentiate BTHS patients from controls.

Bruker
@ Rm4

Pre-Register

Bruker will be presenting two talks during our workshop:

PASER: Parallel Database Search Engine I Real-Time
John R. Yates III
Ernest W. Hahn Professor in the Departments of Molecular Medicine and Neurobiology at The Scripps Research Institute

Clinical Implementation of Mass Spectrometry Imaging
Sankha (Bobby) Basu, MD, PhD
Assistant Director of Clinical Chemistry and Mass Spectrometry and Instructor of Pathology at Harvard Medical School

To view both abstracts please click on the registration link.

For Research Use Only. Not for use in diagnostic procedures.

Agilent Technologies
@ Rm5

Pre-Register

Agilent will be presenting two talks during the lunch seminar:

QA/QC of Large-Scale and High-Coverage Mass Spectrometry-based Lipidomics Analyses
Bo Burla Ph.D.
National University of Singapore

Detection of Chemical Weapon Nerve Agents in Bone
Timothy J. Garrett, PhD
University of Florida

For Research Use Only. Not for use in diagnostic procedures.

1230
1330
Discussion Group: METASPACE
@ Room 3
Chair: Theodore Alexandrov
A workshop to discuss METASPACE.

The METASPACE platform hosts an engine for metabolite annotation of imaging mass spectrometry data as well as a spatial metabolite knowledgebase of the metabolites from hundreds of public datasets provided by the community.

Scientific Session 3

Track 1
Room 1

Fentanyl at the Forefront: Using Non-traditional Approaches to Identify Fentanyl Analogs
Chair: Hema Ketha
2nd Chair: Melissa Hoffman

Track 2
Room 2

Imaging -omics of Hepatic Disease
Chair: Michael Chen

Track 3
Room 3

Microbiology Keynote: Robert Cody
Chair: Chris Cox

Track 4
Room 4

Imaging MS: Translational Research
Chair: Reid Groseclose
2nd Chair: Aalim Weljie

Track 5
Room 5

Proteomics: Established Biomarkers Done Better
Chair: Andrew Hoofnagle

Track 6
Room 6

Practical Training
Chair: Grace van der Gugten

1400
1420
A Semi-targeted Approach for Detecting Fentanyl Analogs in Urine Using Precursor Ion Scan Mode
Stephen Roper
Washington University School of Medicine
Sodium-doped Gold-assisted Imaging Mass Spectrometry Reveals Alterations in Triglyceride Distribution in NAFLD Human Liver as Conditions Progress
Ethan Yang
University of Montreal
The State of the DART: Does Direct Analysis in Real-Time Mass Spectrometry have a Future in Clinical Chemistry?
Robert Cody
JEOL USA, Inc
Deciphering Racial Disparities in Breast Cancer Collagen Re-alignment by Targeted Extracellular Matrix Proteomics
Peggi Angel
Medical University of South Carolina
Measurement of Immature Reticulocyte Proteins in Dried Blood Spots
Holly Cox
Sports Medicine Research and Testing Laboratory
What Do You Do When Your Good Assay Goes Bad?
Sarah Shugarts, Howard Horng
1420
1440
Using High Resolution Mass Spectrometry to Evaluate Trends in Fentanyl Analogues Prevalence and Other False Positives in Emergency Medicine Patients
Adina Badea
University of California - San Francisco
Lipid Profiling and Imaging Predict Proliferation Status of Hepatocellular Carcinoma
Zoe Hall
Imperial College London
...
Keynote, continued
...
Integrating Desorption Electrospray Ionization Mass Spectrometry Imaging and Fluorescence In-situ Hybridization for the Detection of Circulating Tumor Cells
Alena Bensussan
University of Texas at Austin
Innovation and Discovery in Operations: Isotopic Peak Index, Relative Retention Time and Tandem MS for Detection of 15 IGF-1 Variants in Clinical Analysis
Ievgen Motorykin
Quest Diagnostics
...
Extended Session
...
1440
1500
Development of a Fentanyl Analogue Screening Library for High-Resolution Mass Spectrometry
Jada (Yu) Zhang
Zuckerberg San Francisco General Hospital, UCSF
MALDI Imaging Mass Spectrometry of N-Glycan Profiles of Molecular Subclasses of Human Hepatocellular Carcinoma
Andrew Delacourt
Medical University of South Carolina
...
Keynote, continued
...
Imaging Mass Spectrometry of Lipids in Retina Deposits of Aged Human Eyes
Kevin Schey
Vanderbilt University
Automation and Implementation of a Nanobody-MALDI-TOF Platform for Rapid Detection and Isotyping of Monoclonal Gammopathies
Surendra Dasari
Mayo Clinic
...
Extended Session
...
1500
1615
Exhibits & Poster Session
@ Exhibit Hall

Odd 'a' poster positions to be attended.
1515
1600
Meet-a-Mentor: Poster Tours 1
@ Exhibit Hall

Join a mentor as they tour the posters. Learn more about what mentors are looking for in a poster, and how they engage the presenter.
1600
1615
Remove Wed Posters
@ Exhibit Hall

Scientific Session 4

Track 1
Room 1

A 360ᵒ View of Immunosuppressants: Assay Development to Clinical Applications
Chair: Mark Marzinke
2nd Chair: Ganesh Moorthy

Track 2
Room 2

Metabolomics of Small Volume Specimens - Doing More with DBS
Chair: Amy Caudy (pending)

Track 3
Room 3

Data Science Keynote: Andrea Geistanger
Chair: Stephen Master

Track 4
Room 4

Imaging MS: Biomarkers
Chair: Kevin Schey
2nd Chair: Marissa Jones

Track 5
Room 5

Pinky and the Brain Proteomics
Chair: Tim Collier

Track 6
Room 6

Practical Training
Chair: Matt Crawford
2nd Chair: Deborah French

1615
1635
Determination of Sirolimus, Everolimus, Tacrolimus and Cyclosporin in Whole Blood by LC-MS/MS for Therapeutic Drug Monitoring
Natalicia De Jesus Antunes
Queen Mary University of London
A Novel Tandem MS Approach to Dried Blood Spot Analysis of Homocysteine, Methylmalonic Acid, and Methylcitric Acid in the Newborn Screening Laboratory
Joshua Dubland
University of British Columbia
MCR and VCA – Two R Packages to Facilitate Your Method Comparison and Precision Studies
Andrea Geistanger
Roche Diagnostics GmbH
Investigating Cardiolipin Aberrations in Gliomas Using Desorption Electrospray Ionization High Field Asymmetric Ion Mobility Mass Spectrometry Imaging
Anna Krieger
University of Texas at Austin
Characterization of Pre-analytical Variables and their Influence on High-performance Plasma Amyloid-β Biomarkers for Alzheimer’s Disease
James Boslett
University of Pittsburgh
Working Smarter Not Harder on Sample Prep
Matthew Crawford
Labcorp
1635
1655
Accurate Recovery of Immunosuppressant Drugs from Blood: The Tale of a Tortoise and a Hare
Stacy Dee
Laboratory Corporation of America
High Resolution Mass Spectrometry Newborn Screening Applications for Quantitative Analysis of Amino Acids and Acylcarnitines in Dried Blood Spots
Austin Pickens
Centers for Disease Control and Prevention
...
Keynote, continued
...
Spatially-targeted Proteomics for Analysis of Staphylococcus aureus Abscess Formation
Emma Guiberson
Vanderbilt University
Measurement of CSF Hypothalamic Peptides in Frontotemporal Dementia Using LC-MS/MS
Carolin Heller
University College London (UCL)
...
Extended Session
...
1655
1715
Unexpected Toxic Tacrolimus Levels in Pediatric Heart Transplant Patient
Gang Xu
Medical University of South Carolina
Moving Genetic Diseases from a Single Endpoint- to a Pattern-based Diagnosis Using Metabolomics
Anas Abdel Rahman
King Faisal Specialist Hospital and Research Cente
...
Keynote, continued
...
Imaging Mass Spectrometry Analysis of Glycans from Tissue to Serum - Identification and Validation of Biomarkers for Hepatocellular Carcinoma
Anand Mehta
Medical University of South Carolina
Spatio Temporal Proteomic Guided by MALDI MSI Opens the Door of Therapy for Spinal Cord Injury
Michel Salzet
Université De Lille, Inserm U1192
...
Extended Session
...
1715
1830
Exhibits
@ Exhibit Hall
1715
1730
Place Thu Posters
@ Exhibit Hall
1730
1830
Meet-a-Mentor: Office Hours
@ Exhibit Hall

Have a question from the congress that you have been itching to ask? Or a problem from work that you want to get feedback on? Or feedback on the congress? Sign up at the registration desk for 15-min blocks to share time and ideas with a mentor in a relaxed setting.
View Mentors and more info.
1730
1830
Troubleshooting Forum: Poster Rounds
@ Exhibit Hall

Join the troubleshooting poster discussion to learn something new or add your two cents.
17:30 @ poster #93b
Understanding and Managing Matrix and Solvent Instability for Olanzapine
Diane Ly
ARUP Laboratories
17:45 @ poster #94b
Investigation of Range Independent Deviations at High End of the Curve
Koen van der Drift
Darthmouth-Hitchcock Medical Center
18:00 @ poster #95b
Seeing the Invisible; Ion Suppression Resulting from Discrete Pharmaceuticals and Metabolites
Anna Miller
MedTox, Labcorp
18:15 @ poster #96b
Nonspecific Adsorption and Loss of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol. Dude, Where’s My THC?
Triniti Jensen
ARUP Laboratory
1845

Distinguished Contribution Award Plenary Lecture


@ Rooms 4-6
Chair: Distinguished Contribution Award Committee : Shannon Haymond, Marilyn Huestis, Rick Yost, Stephen Master

1845
1930

On the Award

My Path to Enlightenment, or How I Became a Clinical Mass Spectrometrist and Clinical Chemist
Alan Rockwood
Professor Emeritus, University of Utah

1945
2045
FeMS: Award Presentation and Discussion Group
@ Room 1
Chairs: Grace van der Gugten and Hema Ketha
1. Presentation of The Catherine E Costello Award for Females in Mass Spectrometry to Dr. Sarah Brown Riley, sponsored by Avanti Polar Lipids and presented by Catherine E Costello.
2. Small group networking and guided discussion regarding topics relevant to Females in Mass Spectrometry.
1945
2200
No Host Dinner
@ Soleil Pool

Enjoy a pleasant Palm Springs evening with colleagues by the pool where food and drinks will be available for purchase.

Alternatively, if you prefer an offsite meal, you can try nearby:
1. Draughtsman, 3:00pm to 10pm, Food, large selection of Craft Beers, outdoor games. Settle in.
2. Sandfish, Excellent Sushi and whiskey pairings. 4:30 to 10pm. Reservation recommended.
3. Billy Reeds, American comfort food, good for large groups. 8am-9pm.
4. JJ's Latin Oasis, Mexican food.

A wee bit further:
1. Copleys, Fine Dining (reservation)
2. Workshop, Contemporary (reservation)
3. Purple Palm Poolside Mediterranean (reservation)

Thursday

800
845

Corporate Workshop(s)

PerkinElmer
@ Rm1

Achieving the Standard for the Analytical Scope and Sensitivity Testing in Impaired Driving using Laminar Flow Tandem Mass Spectrometry

Sabra Botch-Jones, M.S., M.A., D-ABFT-FT
Assistant Professor, Department of Anatomy & Neurobiology
Boston University School of Medicine

Based on results from the 2017 National Survey on Drug Use and Health, 21.4 million people aged 16 or older operated a vehicle under the influence of alcohol and 12.8 million drove under the influence of illicit drugs in the previous year. Due to the prevalence of driving while impaired, the forensic toxicology scientific community developed ASB Standard 119 “Standard for the Analytical Scope and Sensitivity of Forensic Toxicology Testing in Impaired Driving Investigations”. The scope of the standard provides the minimum requirement for target analytes and analytical sensitivity for the analysis biological fluids such as urine in suspected drugged driving cases.

Chromsystems
@ Rm2

Validation and Implementation of the Chromsystems MassTox Drugs of Abuse Assay on a High Resolution Accurate Mass Spectrometer

Speaker: Dr Sergei Likhodi, Scientific Director, BC Provincial Toxicology Centre, Provincial Health Services Authority, Vancouver, BC, Canada

Dr Likhodi will discuss the experience of his laboratory in implementing the MassTox® Drugs of Abuse Testing assay, a commercial solution that allows the target screening of 108 drugs. He will show validation and performance data, but will also explain how his laboratory successfully expanded the assay with additional drugs analyzed by the same method. Dr Likhodi will demonstrate that the assay, originally developed for LC-MS/MS, can be also established on high resolution mass spectrometers for routine analysis.

Newomics
@ Rm3

Multiply Mass Spectrometry Performance with Multinozzle Emitter Technology
Na Parra, Ph.D., Senior Director of Marketing, Newomics, Berkeley, CA

Nanoflow LC-MS enables high sensitivity analysis of proteins and lipids in biological samples. However, it lacks robustness and throughput. High-flow LC-MS achieves robustness but its sensitivity needs improvement. Microflow LC-MS bridges this gap with a balance of sensitivity and robustness. Newomics award-winning silicon-multinozzl-emitter splits a microflow stream into multiple nanoflows to dramatically enhance ionization efficiency and achieve high sensitivity, robustness, and throughput. The applications of our microflow-LC-nanospray-ESI-MS-platform using Newomics M3 emitter has been demonstrated in proteomics, lipidomics, and native MS.

New Highly Sensitive and Specific Top-Down Mass Spectrometric CDC Reference Procedure for Parathyroid Hormone
Candice Ulmer, Ph.D., Associate Service Fellow, CDC, Atlanta, GA

Parathyroid hormone (PTH) is a key biomarker for chronic-kidney-disease. Measurement of PTH and fragments in serum is critical for disease diagnosis. PTH is at low circulating levels. Current methods show high variability. Thus, a highly sensitive and reliable reference procedure is in need for analysis of PTH and fragments. A top-down proteomics UHPLC-HRMS reference procedure was developed using a Thermo-Q-Exactive-Plus, an UltiMate-3000-RSLCnano, and Newomics M3 emitter. This method allows for serum iPTH measurements from CKD patients.

Waters
@ Rm4

Pre-Register

‘Sensitivity, Selectivity, and Speed’ - Solving Analytical Challenges in Endocrinology using LC-MS/MS for Clinical Research

Presenter: Dominic Foley, Senior Scientist, Waters Corporation, Wilmslow, UK

The use of LC-MS/MS technologies in Endocrinology for clinical research is an area of rapid growth. This is a result of the demonstrated improvements in selectivity provided by the platform compared to more traditional technologies such as immunoassay. In addition, the ability to monitor large number of analytes in a single method, with improvement in both analytical sensitivity and selectivity, increases the speed at which a sample can be interrogated for biochemical abnormalites. In this workshop, we will cover aspects of analytical sensitivity, selectivity and speed in relation to improving the performance of endocrine methods using higher end LC-MS/MS platforms such as the Xevo TQ-S micro and Xevo TQ-XS mass spectrometers.

Restek
@ Rm5

Coated Blade Spray Mass Spectrometry redefines rapid screening and quantitation of small molecules in biological fluids

Presented by
Kara Lynch, PhD; Associate Professor, University of California San Francisco
German Gomez, PhD; R&D Scientist, Restek Corporation

Current workflows for the determination of controlled substances and drugs of abuse in biological fluids rely on sometimes lengthy and complex sample preparations and chromatographic separations. This is particularly challenging in cases where obtaining reliable results rapidly is of prime importance. Undeniably, there is need for highly robust and trustworthy devices that enable rapid detection and quantitation of drugs of abuse in biological fluids, particularly on-site. Coated Blade Spray (CBS) is a technology that combines sample preparation and direct coupling to mass spectrometry (MS) on a single device. Dr. Gomez will briefly introduce the fundamentals behind CBS-MS and Dr. Lynch will describe the challenges clinical researchers experience when performing rapid screening and how CBS-MS can be used as a tool to overcome some of these challenges.

845
900
Intermission
@ Ballroom Foyer

Scientific Session 5

Track 1
Room 1

Targeted Mass Spectrometric Applications for Targeted Populations
Chair: Richard Van Breemen
2nd Chair: Ruben Luo

Track 2
Room 2

Discovery Metabolomics of Clinical Cohorts
Chair: Amy Caudy (pending)

Track 3
Room 3

Data Science: Toxicology
Chair: Lindsay Bazydlo

Track 4
Room 4

Microbiology: New and Experimental Approaches in Microbial Mass Spectrometry
Chair: Chris Cox

Track 5
Room 5

Proteomics Keynote: Michael Lassman
Chair: Mari DeMarco

Track 6
Room 6

Practical Training
Chair: Will Thompson
2nd Chair: Judy Stone

900
920
High Resolution Mass Spectrometry to Quantitatively Determine Modulations in Estrogen Metabolism for Postmenopausal Women Consuming Botanical Dietary Supplement
Alan Wong
Linus Pauling Institute, Oregon State University
MS-based Metabolomics Reveal Bile Acid Metabolism Dysregulation in Malnourished Children from Study of Environmental Enteropathy and Malnutrition
Xueheng Zhao
Cincinnati Children’s Hospital
Creation of a Review by Exception Workflow in DI to Automate Resulting of Interpretive Reports for Confirmatory Opiate Drug Testing
Benjamin Beppler
Tricore Reference Laboratories
Identification of Clinically Relevant Microbes with the MasSpec Pen
Sydney Povilaitis
University of Texas at Austin
The Changing Landscape of Immune-oncology and How Mass Spectrometry Might Help Guide Patient Care
Michael Lassman
Merck & Co
System Suitability and Real-time Quality Control Using Skyline and Panorama
J. Will Thompson, Chris Shuford, Mike MacCoss
920
940
A Method for Quantifying New Anti-TB Drug Delamanid and its Metabolite DM6705 in Patient Hair Samples
Andrew Reckers
University of California San Francisco
A Metabolomic Analysis of the Hispanic Paradox
Jeffrey Patterson
Arizona State University
An Interactive Dashboard for Toxicology Results Using the R Shiny Package
Alex Mays
University of Washington
Development of Metal Oxide Laser Ionization Mass Spectrometry Imaging (MOLI MSI) for Pathogen Detection in Tissues
Madison Mcminn
Northeastern University
...
Keynote, continued
...
...
Extended Session
...
940
1000
Development of a LC-MS/MS Method to Measure Sulfated and Glucruonidated 25OHD3; Comparisons to Unconjugated 25OHD in Circulation
Carl Jenkinson
University of Birmingham
A Novel UPLC-MS Approach for Metabotyping Burn Injury
Elizabeth Want
Imperial College London
Discovering Cross-reactivity in Urine Drug Screening Immunoassays Through Large-scale Analysis of Electronic Health Records
Jake Hughey
Vanderbilt University Medical Center
Next-generation Infection Diagnostics
Vladimir Havlicek
Institute of Microbiology
...
Keynote, continued
...
...
Extended Session
...
1000
1100
Exhibits & Poster Session
@ Exhibit Hall

Even 'b' poster positions to be attended.
1015
1100
Meet-a-Mentor: Poster Tours 2
@ Exhibit Hall

Join a mentor as they tour the posters.

Scientific Session 6

Track 1
Room 1

Tox/TDM/Endocrine Keynote: Thomas Rosano
Chair: Jen Colby

Track 2
Room 2

Glycomics
Chair: Richard Drake

Track 3
Room 3

Microbiology Werkshop
Chair: Chris Cox

Track 4
Room 4

Imaging MS: Therapeutic Targets
Chair: David Muddiman

Track 5
Room 5

Omics Data Science
Chair: Irene van den Broek

Track 6
Room 6

Practical Training
Chair: Zlata Clark
2nd Chair: Jacqueline Hubbard (pending)

1100
1120
Evolving Role of Nominal and High Resolution Mass Spectroscopy in Routine Toxicology Casework
Thomas Rosano
National Toxicology Center; Albany Medical College
Antibody Panel Based N-glycan Profiling by MALDI MS for N-Glycoprotein Biomarker Discovery from Patient Biofluids
Alyson Black
Medical University of South Carolina
Practical Training: On the Utility of Clinical Mass Spectrometry for Microbial Diagnostics
Chris Cox
Cobio Diagnostics
Metabolic Profiling of MYC-induced Tumors by DESI-MSI to Identify Possible Druggable Targets
Katy Margulis
The Hebrew University of Jerusalem
Supervised Data Processing and Quality Control of Mass Spectrometry-based Lipidomics Analyses Using LIDAR, a Novel Toolbox Implemented in R/Shiny
Bo Burla
National University of Singapore
Taking Aim at Analytical Interference in LC-MS Without Shooting Yourself in the Foot
Zlata Clark
ARUP Laboratories
1120
1140
...
Keynote, continued
...
Glycopeptide Biomarkers in Serum Haptoglobin for Hepatocellular Carcinoma Detection in Patients with Non-alcoholic Steatohepatitis
Jianhui Zhu
University of Michigan
...
Extended Session
...
Mass Spectrometry and Immunofluorescence Imaging for Identifying Metabolic Markers of Immunotherapy Response in Syngeneic Tumor Models
Mary King
The University of Texas at Austin
Data-analysis Process for Multiplexed Mass Spectrometry-based Protein Assays
Irene van den Broek
Cedars-Sinai Precision Biomarker Laboratories
...
Extended Session
...
1140
1200
...
Keynote, continued
...
Ultrahigh Resolution MS Profiling of Plasma N-glycans in Large Type 2 Diabetes Cohorts
Elham Memarian
Leiden University Medical Center
...
Extended Session
...
MALDI FTICR MS Imaging and Quantitative Lipidyzer Approach to Investigate Total Parkinson Disease Lipidome and Circulating Lipidomic Biomarkers of PD
E. Ellen Jones
NCTR / FDA
Deep Learning for Prediction and Prospective Validation of Functional Groups for Autonomous Instrumentation
Gaurav Chopra
Purdue University
...
Extended Session
...
1200
1315
Lunch
@ Exhibit Hall

Scientific Session 7

Track 1
Room 1

TBA
Chair: TBA

Track 2
Room 2

Metabolomics Keynote: Theodore Alexandrov
Chair: Adam Rosebrock (pending)

Track 3
Room 3

TBA
Chair: TBA

Track 4
Room 4

New Imaging Hardware/Technology
Chair: Christopher Anderton
2nd Chair: Wojciech Michno

Track 5
Room 5

Monoclonal Ab Therapeutics and MS
Chair: Paula Ladwig

Track 6
Room 6

Practical Training
Chair: Russ Grant
2nd Chair: Grace van der Gugten

1315
1335

CLOSED
Spatial Metabolomics: From Big Data to Single Cells
Theodore Alexandrov
European Molecular Biology Laboratory

CLOSED
Development of <i>in vivo</i> Real-time Molecular Topological Imaging of Cancer Using SpiderMass
Isabelle Fournier
Université De Lille, Inserm, U1192
MS Quantitation and/or Screening of Monoclonal Antibody Therapeutics: the Good, the Bad, the Ugly
Paula Ladwig
Mayo Clinic
Transition Ratio Monitoring for the Masses ... or When to Tell that Your Mass Spectrometer is Lying to You
Russ Grant
Labcorp
1335
1355

CLOSED
...
Keynote, continued
...

CLOSED
New Directions for IR-MALDESI Mass Spectrometry Imaging: From Cells and Bones to Drift Tube Ion Mobility
David Muddiman
North Carolina State University
...
Extended Session
...
...
Extended Session
...
1355
1415

CLOSED
...
Keynote, continued
...

CLOSED
Characterizing Spatial-Temporal Abeta Plaque Aggregation Dynamics in Evolving AD Pathology Using iSILK
Wojciech Michno
Sahlgrenka Academy at the University of Gothenburg
...
Extended Session
...
...
Extended Session
...
1315
1415
Exhibitor Feedback Meeting
@ Room 1
Chair: Chris Herold
Exhibitors, please have at least one representative attend this important opportunity to shape the future editions of MSACL.
1415
1530
Exhibits & Poster Session
@ Exhibit Hall

Odd 'b' poster positions to be attended. Poster Finalists to attend their posters for final interviews.
1515
1530
Remove Posters
@ Exhibit Hall

You can store your poster at the Reg Desk until 20:00.
1530
Exhibits Closed
@ Exhibit Hall

Scientific Session 8

Track 1
Room 1

Practical Training
Chair: Shannon Haymond
2nd Chair: Judy Stone

Track 2
Room 2

New Applications and Strategies
Chair: Livia Eberlin
2nd Chair: Anand Mehta

Track 3
Room 3

Closed
Chair: TBA

Track 4
Room 4

Keynote: Catherine Costello
Chair: David Muddiman

Track 5
Room 5

Closed
Chair: TBA

Track 6
Room 6

Closed
Chair: TBA

1530
1550
Working with R for Validation Summaries in the Clinical Laboratory
Lindsay Bazydlo
University of Virginia
Molecular Diagnostics of Esogastric Cancer with SpiderMass Real-time Analysis: A Step Closer to in vivo Application
Nina Ogrinc
Laboratory Prism, University of Lille

CLOSED
Emerging Opportunities for Incorporation of Proteomics and Glycoproteomics into Clinical Analyses
Catherine Costello
Boston University School of Medicine

CLOSED

CLOSED
1550
1610
Putting R Skills to Use in Clinical Laboratories
Dustin Bunch
Northwestern University Feinberg School Medicine
An In-situ Single Cell Atlas of the Terminal Bronchioles in Chronic Obstructive Pulmonary Disease by Imaging Mass Cytometry
Tillie Hackett
Center for Heart Lung Innovation, Ubc

CLOSED
...
Keynote, continued
...

CLOSED

CLOSED
1610
1630
No Middleware? No Problem. Using R and Shiny for Routine Review of QC Data and Other Quality Metrics
Dennis Orton
Alberta Precision Laboratories
Simultaneous MS Imaging of Multiple Brain Signaling Molecules and Alterations Induced by Parkinsonism and L-DOPA Therapy
Per Andren
Uppsala University

CLOSED
...
Keynote, continued
...

CLOSED

CLOSED
1630
1645
Plenary Reception
@ Ballroom Foyer
1645
1700
Poster Awards
@ Rooms 4-6

Chair: Cory Bystrom
Join us in congratulating the winners of the poster contest.
1700

Plenary Lecture


@ Rooms 4-6
Chair: David Herold

1700
1745
Brain Organoids as a Model System for Human Neurodevelopment and Evolution
Alysson Muotri
University of California, San Diego

Structural and transcriptional changes during early brain maturation follow fixed developmental programs defined by genetics. However, whether this is true for functional network activity remains unknown, primarily due to experimental inaccessibility of the initial stages of the living human brain. We developed cortical organoids that spontaneously display periodic and regular oscillatory network events that are dependent on glutamatergic and GABAergic signaling. These nested oscillations exhibit cross-frequency coupling, proposed to coordinate neuronal computation and communication. As evidence of potential network maturation, oscillatory activity subsequently transitioned to more spatiotemporally irregular patterns, capturing features observed in preterm human electroencephalography (EEG). These results show that the development of structured network activity in the human neocortex may follow stable genetic programming, even in the absence of external or subcortical inputs. Our approach provides novel opportunities for investigating and manipulating the role of network activity in the developing human cortex. Applications for neurodevelopmental disorders and brain evolution will be discussed.

1745
1800
Final Remarks
@ Rooms 4-6

Chairs: Dan Holmes and Cory Bystrom
The Scientific Committee Chair and Vice Chair wrap up MSACL and share information about upcoming Conferences. IMSS Special Topic Scientific Sessions continue through Friday morning.
1930
2200
Hospitality Lounge
@ Gypsy Rose

Friday

830
1030
Scientific Committee Leads 2021 Planning Meeting
@ Room 4
Chair: Amber Herold
Closed meeting for SciCom Leads and Admin to prepare for 2021!

Scientific Session 9

Track 1
Room 1

Imaging Mass Spectrometry Society Special Session I
Chair: Chris Anderton
2nd Chair: Jessica Lukowski

Track 2
Room 2

TBA
Chair: TBA

Track 3
Room 3

TBA
Chair: TBA

Track 4
Room 4

TBA
Chair: TBA

Track 5
Room 5

TBA
Chair: TBA

Track 6
Room 6

TBA
Chair: TBA

830
850
The Chemical Characterization of the Cells in the Brain Using Mass Spectrometry
Jonathan Sweedler
University of Illinois at Urbana-Champaign

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
850
910
...
Keynote, continued
...

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
910
930
Gold Nanoparticles as a New Clinical Alternative for Mass Spectrometry Imaging
Katherine Stumpo
University of Scranton

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
930
945
Intermission
@ Ballroom Foyer

Scientific Session 10

Track 1
Room 1

Imaging Mass Spectrometry Society Special Session II
Chair: Chris Anderton
2nd Chair: Jessica Lukowski

Track 2
Room 2

TBA
Chair: TBA

Track 3
Room 3

TBA
Chair: TBA

Track 4
Room 4

TBA
Chair: TBA

Track 5
Room 5

TBA
Chair: TBA

Track 6
Room 6

TBA
Chair: TBA

945
1005
A New Enzymatic Approach to Distinguish Fucosylation Isomers of N-linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry
Connor West
Medical University of South Carolina

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
1005
1025
Multiple Instance Learning for Classification in Mass Spectrometry Imaging
Dan Guo
Northeastern University

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
1025
1045
Co-registration of Confocal Fluorescence Images and MALDI MSI Facilitates Prediction of Pluripotency Status in Human iPSC Colonies
Arina Nikitina
Georgia Institute of Technology

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
1045
1100
Intermission
@ Ballroom Foyer

Scientific Session 11

Track 1
Room 1

Imaging Mass Spectrometry Society Special Session III
Chair: Chris Anderton
2nd Chair: Jessica Lukowski

Track 2
Room 2

TBA
Chair: TBA

Track 3
Room 3

TBA
Chair: TBA

Track 4
Room 4

TBA
Chair: TBA

Track 5
Room 5

TBA
Chair: TBA

Track 6
Room 6

TBA
Chair: TBA

1100
1120
A Quantitative Investigation of the Effects of Spray Parameters on Crystal Size, Analyte Delocalization, and Signal Intensity
Sloane Tilley
HTX Technologies

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
1120
1140
New Technologies for Chemical Profiling and Imaging of Thin Tissue Sections and of Single Cells
Vilmos Kertesz
Oak Ridge National Laboratory

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
1140
1200
Exploring Molecular Interactions within Environmental Microbiomes Using Mass Spectrometry Imaging
Christopher Anderton
Pacific Northwest National Lab

CLOSED

CLOSED

CLOSED

CLOSED

CLOSED
1200
1215
Closing Remarks
@ Room 1

Chair: Chris Anderton
Wrap up of IMSS Special Topics and information about upcoming Conferences.
1215
Conference Closed
@ Ballroom Foyer