Cyrus Papan (Presenter)
AB SCIEX Germany
Authorship: Cyrus Papan 1; Baljit K. Ubhi2; Susan Fisher3, and Katherine Williams3
(1) AB SCIEX GmbH, Germany; (2) SCIEX, Redwood City, USA; (3) Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, USA
| Short Abstract Preeclampsia, the onset of hypertension and proteinuria at =20 weeks of gestation, is a leading cause of maternal perinatal morbidity and mortality. Since preeclampsia is known to affect lipid metabolic pathways, we set out to validate and quantitate the plasma lipidome from women with severe preeclampsia (sPE) and preterm labor (PTL). Using the LipidyzerTM Platform (SCIEX) allowed profiling and accurate quantitation of approximately 1100 lipid molecular species from 13 different lipid classes. Lipid profile abnormalities were found in the triacylglycerides (TAGs) and diacylglycerides species (DAGs) as well as some novel markers found to increase in Preeclampsia. |
Long Abstract
Introduction
Preeclampsia, the onset of hypertension and proteinuria at ≥20 weeks of gestation, is a leading cause of maternal perinatal morbidity and mortality. Despite significant recent efforts to define biomarkers to improve the ability of clinicians to predict, diagnose and manage preeclampsia, none perform well enough to have broad clinical utility. Preeclampsia is known to affect lipid metabolic pathways, e.g., abnormally elevated triglycerides, total cholesterol and low density lipoprotein cholesterol have been found to be associated with preeclampsia. We quantified the plasma lipidome from women with preeclampsia or preterm labor using the Lipidyzer TM Platform (Sciex). Lipid profile abnormalities were found in the triacylglycerides species (TAGs) and diacylglycerides species (DAGs) as well as some novel markers found to increase in Preeclampsia.
Methods
Plasma from women with sPE (gestational age 25-37 weeks) were analyzed. Gestational age-matched plasma samples from women with preterm birth (PTL) were used as controls. Applying internal standards (Avanti Polar Lipids), plasma was extracted using a modified Bligh/Dyer protocol. The Lipidyzer TM Platform (SCIEX) was used for targeted profiling of approximately 1100 lipid molecular species from 13 different lipid classes for comprehensive coverage of the plasma lipidome. This strategy allowed for: i) quantitative results for each lipid class as a sum of individual species; ii) mole percent composition obtained computationally from lipid molecular species data; and iii) accurate lipid species compositions. The data were automatically processed generating heat maps allowing facile data analysis and biological data interpretation.
Preliminary Data
We analyzed over one thousand lipid molecular species; coefficient of variations (CVs) for all species was < 0.06%, highlighting excellent reproducibility of the assay. Plasma from women with severe preeclampsia primarily showed differences in the TAG and DAG species as compared with preterm labor controls. The data were probed for pathway interactions and statistical analyses were conducted in Surveyor Web Tools (Metabolon, USA). A novel set iof lipid molecular species were found to increase significantly, namely PC(16:0/14:1), LPC(18:3), CE(18:0), TAG50:5-FA20:5 and CE(20:1). A subset of these markers which increase and the TAG and DAG molecular species which decreased could be used to validate these potential markers in parallel sample cohorts to better predict, diagnose and manage preeclampsia.
Novel Aspect
Differential Complex Lipid Plasma Profiles Reveal Perturbations in Preeclampsia.
References & Acknowledgements:
| Description | Y/N | Source |
| Grants | no | |
| Salary | yes | AB SCIEX Gmbh |
| Board Member | no | |
| Stock | no | |
| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | no |