Songlin Yu (Presenter)
Peking Union Medical College Hospital
Bio: I am a young scientist working in the department of laboratory medicine, Peking Union Medical College Hospital in China, and my research area is about method establishment based on HPLC, LC-MS/MS, ICP-MS.I have developed and validated methods for fatty acids, 25OHD, and iodine for clinical detection.
Authorship: Songlin Yu, Qian Cheng, Ling Qiu
(1) Peking Union Medical College Hospital
| Short Abstract Whether thyroid disease is associated with fortified iodine in the salt in China was unclear because of lacking efficient method for iodine measurement. This paper aims at establishing a simple and quick method for iodine determination in human urine and serum by ICP-MS. The method only needs 30s for analysis of one sample. It was sensitive with a lower limit detection of 0.87 ug/L. Compared with NIST standard reference material 3668, the bias was less than 5%. The inter-coefficient variation (CV) for serum and urine iodine was 1.2%~3.0%, 2.0%~2.9%, respectively; and total CV was 3.0%~3.8%, 4.1%~4.9%, respectively. The urine and serum iodine of Chinese pregnant women was 154.8±89.7 ug/L (mean±SD),75.8±21.4 ug/L, respectively. In conclusion, a rapid, simple and accurate ICP-MS method that can be used in clinical laboratory for urine and serum iodine measurement has been established. |
Long Abstract
Introduction: Thyroid diseases in China are prevalent; however, whether they are associated with fortified iodine in the salt was controversy because of lacking efficient method for the iodine measurement. This paper aims at establishing a method for quantification of iodine in human urine and serum by inductively coupled plasma mass spectrometry (ICP-MS), and providing an assay that can be used to the evaluation of the level of iodine in routine clinical laboratory.
Methods: Ammonia, isopropanol and ultrapure water was mixed to prepare for the diluent solution which was used to dilute samples in the ratio of 1:10, and then the diluted sample was analyzed by ICP-MS. Rhenium was used as the internal standard. Linearity, lower limit of detection, recovery, precision, accuracy, carryover and stability was evaluated thoroughly. Iodine levels of pregnant women who required iodine tests were retrospectively analyzed to evaluate the status of iodine in pregnant women.
Results: The method only needs 30s for analysis of one sample. It was sensitive with a lower limit detection of 0.87 ug/L, the correlation coefficient was higher than 0.9999 in ten measurements. The recovery in both serum and urine was approximately 100%. Compared with NIST standard reference material 3668, the bias was less than 5% which showed that the method had good accuracy. The inter-coefficient variation (CV) for serum and urine iodine was 1.2%~3.0%, 2.0%~2.9%, respectively; and total CV for serum and urine iodine were 3.0%~3.8%, 4.1%~4.9%, respectively. The mean carryover of this method was 0.03% and iodine was stable for at least one month at -20°C and 4°C. The urine and serum iodine for pregnant women was 154.8±89.7 ug/L (mean±SD),75.8±21.4 ug/L, respectively. The correlation between urine and serum iodine was 0.21.
Conclusion: A rapid and simple ICP-MS method for urine and serum iodine measurement has been established. It is accurate and precise and can be used in the evaluation of iodine in routine clinical laboratory.
References & Acknowledgements:
1.Lazarus JH. The importance of iodine in public health. Environ Geochem Health. Aug 2015;37(4):605-618.
2.Hetzel BS. Towards the global elimination of brain damage due to iodine deficiency--the role of the International Council for Control of Iodine Deficiency Disorders. Int J Epidemiol. 2005;34(4):762-764.
3.Ding Y, Xing J, Fang Y, Wang Y, Zhang Y, Long Y. 131I therapy for 345 patients with refractory severe hyperthyroidism: Without antithyroid drug pretreatment. Exp Biol Med (Maywood). 2015, in press.
4.Teng W, Shan Z, Teng X, et al. Effect of iodine intake on thyroid diseases in China. N Engl J Med. 2006;354(26):2783-2793.
5.Zimmermann MB, Andersson M. Assessment of iodine nutrition in populations: past, present, and future. Nutr Rev. 2012;70(10):553-570.
6.Michalke B, Witte H. Characterization of a rapid and reliable method for iodide biomonitoring in serum and urine based on ion chromatography-ICP-mass spectrometry. J Trace Elem Med Biol. 2015;29:63-68.
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