Chia-Ni Lin (Presenter)
Chang Gung Memorial Hospital
Authorship: Chia-Ni Lin, I-Wen Wu, Yu-Fen Huang, Ya-Ching Huang, Hsiao-Chen Ning
Chang Gung Memorial Hospital
| Short Abstract Chronic kidney disease causes tremendous impact because it increased risk of cardiovascular disease and mortality. Loss of kidney function induces accumulation of potentially toxic compounds, resulting in uremic retention. Indoxyl sulfate and p-cresyl sulfate are important protein-bound uremic solutes which can stimulate the progression of chronic kidney disease. A sensitive ultra-performance liquid chromatography-tandem mass spectrometry method for quantitation of indoxyl sulfate and p-cresyl sulfate in serum has been developed and can be used to follow-up the progression of disease in chronic kidney disease patients. |
Long Abstract
Chronic kidney disease (CKD) causes tremendous impact because it increased risk of cardiovascular disease and mortality. Loss of kidney function induces accumulation of potentially toxic compounds, resulting in uremic retention. Indoxyl sulfate (IS) and p-cresyl sulfate (pCS) are important protein-bound uremic solutes which can stimulate the progression of CKD. The aim of this study was to develop ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for quantitation of IS and pCS in serum and investigate the levels of IS and pCS in different stage of CKD patients. Stable isotope-labelled IS and pCS were used as internal standards. After protein precipitation, samples were separated on a C18 column followed by negative electrospray ionization and tandem mass spectrometric detection. The limit of detection for IS and pCS were 0.05 and 0.11 µ g/mL, respectively, with a linear calibration range of up to 10 µ g/mL. The intra-day and inter-day coefficient of variation was below 6.7 % for both compounds. This method showed no carryover or ion suppression/enhancement. The applicability of the method was evaluated by analyzing 42 serum samples from patients at different stages of CKD. Good correlation was obtained with the concentrations of both analytes and the progression of CKD stages. In conclusion, a sensitive UPLC-MS/MS method for quantitation of IS and pCS in serum has been developed and can be used to follow-up the progression of disease in CKD patients.
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