Jentaie Shiea (Presenter)
National Sun Yat-Sen University
Bio: Jentaie Shiea received his bachelor degree in Chemistry from National Chung-Hsing University, Taiwan in 1981. He received M.S. (1988) and Ph.D. (1991) degrees from Montana State University (MSU). His thesis studies focus on Organic Geochemistry and Analytical/Physical Chemistry. After post-doctoral fellowship at Pennsylvania State University (Department of Material Science), he joined National Sun Yat-Sen University (NSYSU, Kaohsiung, Taiwan) faculty. He is now a distinguish professor of Department of Chemistry at NSYSU.
Authorship: Jentaie Shiea, Kun-Da Wu, Hung Su
National Sun Yat-Sen University, Kaohsiung, Taiwan
| Short Abstract TD-ESI/MS was employed to characterize caffeine, lamisil, acetaminophen, and plasticizers released on skin. The metal sampling probe has been designed in such a way that it is easily to collect samples directly from skin as well as mass spectrometric detection without any treatment. After the volunteers taking the drug for a period of time, the drug distribution throughout the whole body was studied. Taking lamisil, an anti-fungus drug, as an example; the ion signal of lamisil was found mostly on the upper body especially face.In addition, pharmacokinetic profile of a drug can be determined by analyzing the samples collected from skin with TD-ESI/MS. No blood withdrawing or urine collection is needed. The phthalates released on skin were studies to evaluate the level of an individual exposed to such environmental hormones. |
Long Abstract
Skin, the largest organ in human beings, plays a key role in protecting the human body against different pathogens in the environment. Many metabolites are released onto the skin surface at all the time since numerous glands are distributed right under the skin. Although some of the metabolites on skin are potential disease biomarkers, current analytical techniques do not provide proper way to efficiently identify them on skin. In this study, a novel ambient mass spectrometric technique - thermal desorption electrospray ionization/mass spectrometry (TD-ESI/MS) was used to characterize the chemical and biological compounds including drugs, lipids, and metabolites on skin without sample pretreatment. A stainless steel probe was used to scrap the skin surface for sampling. It was then inserted into a heated oven to thermally desorb the analytes on it. The analytes were subsequently carried by a nitrogen stream to join in an ESI plume (from the solution of methanol/water=1:1 with 0.1% TFA), they were ionized by reacting with the charged species (H+, H3O+, etc) in the ESI plume. The remaining samples were rapidly removed by burning the probe with a flame from a torch. It took less than 30 seconds to complete an analysis.
TD-ESI/MS was employed to characterize caffeine, lamisil, acetaminophen, and plasticizers released on skin. The metal sampling probe has been designed in such a way that it is easily to collect samples directly from skin as well as mass spectrometric detection without any treatment. Moreover, the probe is reusable by burning it with a high-temperature flame. After the volunteers taking the drug for a period of time, the drug distribution throughout the whole body was studied. Taking lamisil, an anti-fungus drug, as an example; the ion signal of lamisil was found mostly on the upper body especially face. This may due to different sweating rates in different skin parts as well as the distribution of veins over the body. In addition, pharmacokinetic profile of a drug can be determined by analyzing the samples collected from skin with TD-ESI/MS. No blood withdrawing or urine collection is needed. The phthalates released on skin were studies to evaluate the level of an individual exposed to such environmental hormones.
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