Shahana Huq (Presenter)
Phenomenex
Bio: Shahana Huq is a senior scientist within the Phenologix applications group at Phenomenex. She holds a MS in Biochemistry from California State University, Los Angeles. Her work involves analytical method development, optimization and troubleshooting. She has broad base of experience in product and application development in sample clean up technologies and equipment including HPLC, mass spectrometers and robotic liquid handlers. She has been working for Phenomenex since the year 2000.
Authorship: Shahana Wahab Huq; Seyed Sadjadi
Phenomenex Incorporated
| Short Abstract Nutritional supplements like vitamins and minerals are critical measure of a patients overall health condition that underwent a gastric bypass and other types of bariatric surgery. In order to quantify the nutritional intake of a patient, we have developed a simple and reliable method to extract vitamin A & E from human serum, using Novum SLE. A Kinetex 5u, Evo C18, 100x2.1 mm HPLC column was utilized to obtain the best selectivity of the two vitamer of vitamin E, alpha and gamma tocopherol along with vitamin A, while a polarity switching technique in mass spectrometric ionization was employed. |
Long Abstract
Nutritional supplements like vitamins and minerals are critical measure of a patients overall health condition that underwent a gastric bypass and other types of bariatric surgery. The dramatic alteration of body’s ability to absorb these important supplements and resulting deficiency may have serious medical implications and therefore should be monitored closely. In order to quantify the nutritional intake of a patient, we have developed a simple and reliable method to extract vitamin A & E from human serum, using Novum SLE in conjunction with Kinetex 5u, Evo C18, 100x2.1 mm HPLC column. A fast and effective LC/MS/MS method was developed to obtain best selectivity of the vitamin A and E along with its two vitamers alpha and gamma Tocopherol. A cleaner background was observed for both alpha and gamma under negative ionization mode. By taking advantage of the polarity switching technique (from positive to negative ionization) after vitamin A elutes from the column, a much cleaner background, resolved in higher signal for both vitamers of Vitamin E under APCI mode.
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